BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_N10 (650 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone... 36 0.005 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 31 0.11 SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 30 0.25 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 29 0.44 SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 29 0.77 SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 29 0.77 SPBC83.16c |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.3 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 2.3 SPAC57A7.06 |||U3 snoRNP protein Utp14 |Schizosaccharomyces pomb... 27 3.1 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 3.1 SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 26 4.1 SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr 2|||M... 26 4.1 SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 26 4.1 SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc... 26 4.1 SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosacchar... 26 5.4 SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 25 7.2 SPCC338.07c |||NatA N-acetyltransferase complex subunit |Schizos... 25 7.2 SPAC664.01c |swi6|SPAC824.10c|chromodomain protein Swi6|Schizosa... 25 9.5 >SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component |Schizosaccharomyces pombe|chr 3|||Manual Length = 809 Score = 35.9 bits (79), Expect = 0.005 Identities = 19/63 (30%), Positives = 38/63 (60%) Frame = +1 Query: 136 PXDDNNEDITEEYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENK 315 P + D E ++LE D S+L+ L+R+N+++ +QLK + NI++++++ E Sbjct: 627 PAKHSRVDRYEHPKVLEDN-DSTISSLESLKRENEELREQLKVEHEENISMQKQLSELKG 685 Query: 316 EVN 324 E+N Sbjct: 686 ELN 688 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 31.5 bits (68), Expect = 0.11 Identities = 16/62 (25%), Positives = 33/62 (53%), Gaps = 1/62 (1%) Frame = +1 Query: 148 NNEDITE-EYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVN 324 NNE + E E+L + +DELNS LE + + + L + ++++E ++++ Sbjct: 20 NNEGLDGIEQEILAAGIDELNSRTRLLENDIKVMKSEFQRLTHEKSTMLEKIKENQEKIS 79 Query: 325 NS 330 N+ Sbjct: 80 NN 81 >SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1208 Score = 30.3 bits (65), Expect = 0.25 Identities = 22/65 (33%), Positives = 35/65 (53%), Gaps = 9/65 (13%) Frame = +1 Query: 130 EPPXDDNNEDITEEYEL--LESTLDELNSALD----FLERKNDDI---HQQLKELLQSNI 282 E D +N+++ EYE+ L++ LDEL+ LD L K D+I +Q++E S+ Sbjct: 271 EMEEDKSNKEVDYEYEIRQLQNRLDELSEELDVAQDLLTEKEDEIATLKRQIEEKENSSS 330 Query: 283 AIRQE 297 A E Sbjct: 331 AFENE 335 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 29.5 bits (63), Expect = 0.44 Identities = 14/58 (24%), Positives = 30/58 (51%) Frame = +1 Query: 154 EDITEEYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNN 327 +D EE+ L LD++ + + KN D+ +L++ N ++ + +NK++ N Sbjct: 701 KDFIEEHSKLTKQLDDIKNQFGIISSKNRDLLSELEKSKSLNNSL-AALESKNKKLEN 757 >SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 28.7 bits (61), Expect = 0.77 Identities = 12/57 (21%), Positives = 29/57 (50%) Frame = +1 Query: 157 DITEEYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNN 327 + TE+ + + + LER+ DD+ Q+L+E+ ++ E+ E ++ + + Sbjct: 104 ETTEKMRQTDVKAEHFERRVQSLERERDDMEQKLEEMTDKYTKVKAELDEVHQALED 160 >SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 952 Score = 28.7 bits (61), Expect = 0.77 Identities = 12/61 (19%), Positives = 33/61 (54%) Frame = +1 Query: 166 EEYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNNSN*RNA 345 E+ + +++++E+ + L+ + + + Q+K LL+ N ++++ + + N NA Sbjct: 308 EKLSIAQNSINEIQTQNRDLKLETEKLQDQIKALLERNQSLQEALETVKNDEKNLREMNA 367 Query: 346 N 348 N Sbjct: 368 N 368 Score = 28.7 bits (61), Expect = 0.77 Identities = 14/44 (31%), Positives = 26/44 (59%) Frame = +1 Query: 202 LNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNNSN 333 L S + E + +H L+ L Q+N +++ E+ E+N E+N+ N Sbjct: 520 LKSQIRDQELTIEKLHDSLETLSQTNNSLQCEISEKNAELNSVN 563 Score = 27.5 bits (58), Expect = 1.8 Identities = 15/59 (25%), Positives = 26/59 (44%) Frame = +1 Query: 151 NEDITEEYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNN 327 N D+ E E L+ + L L+ + + K L + N EM+E +++NN Sbjct: 324 NRDLKLETEKLQDQIKALLERNQSLQEALETVKNDEKNLREMNANYETEMKEARQKLNN 382 >SPBC83.16c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 563 Score = 27.9 bits (59), Expect = 1.3 Identities = 12/50 (24%), Positives = 27/50 (54%) Frame = +2 Query: 227 SVKMMIFTNN*KSFCNPILQLDKKCVKKIRKSTTVIEEMQIQHYFKFNCA 376 S+ +M+ + FC+ + K V K+RK+ ++ ++ +H+ F+ A Sbjct: 103 SIGLMLLLSALIGFCSESIVTSVKSVYKLRKAHSIFSKINKRHFDHFSAA 152 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 27.1 bits (57), Expect = 2.3 Identities = 16/64 (25%), Positives = 33/64 (51%), Gaps = 3/64 (4%) Frame = +1 Query: 145 DNNEDITEEYELLEST---LDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENK 315 D+ E++ + LE +DELNSA L ++ ++ +L + N +++++ Sbjct: 482 DSQEELLQLNAKLEKANIVIDELNSAKLKLSKQVEEESSMKDDLTEMNQRLKEQIESYEN 541 Query: 316 EVNN 327 EVN+ Sbjct: 542 EVNS 545 >SPAC57A7.06 |||U3 snoRNP protein Utp14 |Schizosaccharomyces pombe|chr 1|||Manual Length = 929 Score = 26.6 bits (56), Expect = 3.1 Identities = 14/46 (30%), Positives = 22/46 (47%) Frame = +1 Query: 127 IEPPXDDNNEDITEEYELLESTLDELNSALDFLERKNDDIHQQLKE 264 I PP NN+ T++ LLES N + L D++++ E Sbjct: 160 IRPPWIGNNDHATDKENLLESDASSSNDSESELTDSADNMNESDSE 205 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 26.6 bits (56), Expect = 3.1 Identities = 16/62 (25%), Positives = 33/62 (53%), Gaps = 1/62 (1%) Frame = +1 Query: 160 ITEEYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIA-IRQEMREENKEVNNSN* 336 + EE E L+ +++ N+ L ++ + LK+ + A ++QE+ +NKE+ + N Sbjct: 1392 LNEEVENLKKEVEQANTKNTRLAAAWNEKCENLKKSSLTRFAHLKQELTNKNKELTSKNA 1451 Query: 337 RN 342 N Sbjct: 1452 EN 1453 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/41 (29%), Positives = 23/41 (56%) Frame = +1 Query: 175 ELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQE 297 EL +S + + A + ++ H+Q K L++S I+ R+E Sbjct: 963 ELEKSNIQQKYLASEKTLEMMNETHEQFKHLVESEISTREE 1003 >SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe|chr 2|||Manual Length = 882 Score = 26.2 bits (55), Expect = 4.1 Identities = 13/35 (37%), Positives = 23/35 (65%), Gaps = 1/35 (2%) Frame = +1 Query: 163 TEEYE-LLESTLDELNSALDFLERKNDDIHQQLKE 264 T+ YE L+ +LD +++ F+++ DDI + LKE Sbjct: 844 TKLYERALQQSLDTISANSSFIDKSLDDITRWLKE 878 >SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 26.2 bits (55), Expect = 4.1 Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 3/54 (5%) Frame = +1 Query: 154 EDITEEYELLESTLDELNSALDFLERKN---DDIHQQLKELLQSNIAIRQEMRE 306 E TEE EL + +N+ FL+++N D + + EL + N A E+ + Sbjct: 2 EKDTEELELENAVFGNINNFSSFLDKENETFDVMMNEAPELSEDNDAQEDELEK 55 >SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces pombe|chr 3|||Manual Length = 1526 Score = 26.2 bits (55), Expect = 4.1 Identities = 14/28 (50%), Positives = 19/28 (67%) Frame = +1 Query: 181 LESTLDELNSALDFLERKNDDIHQQLKE 264 L T ELNSALD +++ +IH +LKE Sbjct: 1346 LVDTRKELNSALDSCKKREAEIH-RLKE 1372 >SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 26.2 bits (55), Expect = 4.1 Identities = 12/55 (21%), Positives = 27/55 (49%), Gaps = 2/55 (3%) Frame = +2 Query: 446 YHLSIFSDQYHKVSVSF--K*CIIIHLRYLSMNNYLSCLHHLNVISCIFMSIGMN 604 Y++S Y+ + + F + +++HL +++ HH+ + I +S G N Sbjct: 195 YYMSPLFKAYYLIQLGFWLQQILVLHLEQRRADHWQMFAHHIVTCALIILSYGFN 249 >SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 25.8 bits (54), Expect = 5.4 Identities = 11/36 (30%), Positives = 21/36 (58%) Frame = +1 Query: 226 ERKNDDIHQQLKELLQSNIAIRQEMREENKEVNNSN 333 +R+N Q +++L +SN+ + + E K +NSN Sbjct: 550 KRRNSGTEQVVRQLSKSNVEVIGKGGERKKVASNSN 585 >SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces pombe|chr 1|||Manual Length = 511 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/42 (33%), Positives = 25/42 (59%) Frame = +1 Query: 202 LNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNN 327 +++ L+ LE DD + +LKE NIA +QE+ E+++ Sbjct: 433 VDAYLNPLEFNFDDKNVKLKEFGSPNIAYKQELNSFQGELSS 474 >SPCC338.07c |||NatA N-acetyltransferase complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 729 Score = 25.4 bits (53), Expect = 7.2 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +1 Query: 169 EYELLESTLDELNSALDFLERKNDDIHQ 252 EYEL S ++E S + L +N D HQ Sbjct: 230 EYELYLSKMEEAKSTIYLLLDRNPDNHQ 257 >SPAC664.01c |swi6|SPAC824.10c|chromodomain protein Swi6|Schizosaccharomyces pombe|chr 1|||Manual Length = 328 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/36 (36%), Positives = 22/36 (61%) Frame = +1 Query: 133 PPXDDNNEDITEEYELLESTLDELNSALDFLERKND 240 P DN E ++ E +S ++L S++D +ERK+D Sbjct: 250 PKLPDNRELTVKQVENYDSW-EDLVSSIDTIERKDD 284 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,079,189 Number of Sequences: 5004 Number of extensions: 37600 Number of successful extensions: 146 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 146 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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