Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_M13
(599 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g46560.1 68418.m05733 expressed protein 31 0.44
At5g56840.1 68418.m07092 DNA-binding family protein contains Pfa... 28 4.1
At5g47680.1 68418.m05886 expressed protein contains Pfam profile... 28 4.1
At5g67200.1 68418.m08471 leucine-rich repeat transmembrane prote... 28 5.4
At5g01140.1 68418.m00018 hypothetical protein contains Pfam prof... 28 5.4
At3g54660.1 68416.m06048 gluthatione reductase, chloroplast near... 28 5.4
At5g22355.1 68418.m02608 DC1 domain-containing protein contains ... 27 9.5
At3g08730.1 68416.m01015 serine/threonine protein kinase (PK1) (... 27 9.5
At3g08720.2 68416.m01014 serine/threonine protein kinase (PK19) ... 27 9.5
At3g08720.1 68416.m01013 serine/threonine protein kinase (PK19) ... 27 9.5
At1g74180.1 68414.m08591 leucine-rich repeat family protein cont... 27 9.5
At1g23970.2 68414.m03027 expressed protein contains Pfam profile... 27 9.5
At1g23970.1 68414.m03026 expressed protein contains Pfam profile... 27 9.5
>At5g46560.1 68418.m05733 expressed protein
Length = 378
Score = 31.5 bits (68), Expect = 0.44
Identities = 20/62 (32%), Positives = 25/62 (40%)
Frame = -1
Query: 383 VLITEMLVHHCFNYLSPSLIVRNETNFCDFNLNFFCTCLSSCTFSGCVEYRSCVYGSNSC 204
VL+ V N+LS SL +FCD N N + L C C C G C
Sbjct: 51 VLLVACAVAFTCNFLSKSLSSNPSKSFCDSNFNPIDSDLDIC--EPCPINGECYQGKLQC 108
Query: 203 NI 198
N+
Sbjct: 109 NL 110
>At5g56840.1 68418.m07092 DNA-binding family protein contains Pfam
domains, PF00249: Myb-like DNA-binding domain and
PF00098: Zinc knuckle
Length = 233
Score = 28.3 bits (60), Expect = 4.1
Identities = 14/31 (45%), Positives = 19/31 (61%), Gaps = 2/31 (6%)
Frame = +2
Query: 398 RXCVSYE--VLQMGGLHG*GCSQSPPGDPPP 484
R C SY+ V+++ G+H S SPP PPP
Sbjct: 16 RTCSSYQTRVVRLFGVHLDTTSSSPPPPPPP 46
>At5g47680.1 68418.m05886 expressed protein contains Pfam profile
PF04243: Protein of unknown function (DUF425)
Length = 344
Score = 28.3 bits (60), Expect = 4.1
Identities = 15/40 (37%), Positives = 26/40 (65%)
Frame = +1
Query: 238 STQPEKVQELRHVQKKFKLKSQKLVSFRTIKEGER*LKQW 357
+ Q +++++ R+ KK + K+Q+ R KEGER LK+W
Sbjct: 32 NAQKKQLKQQRYEAKKAEKKAQEKEHKR--KEGERKLKEW 69
>At5g67200.1 68418.m08471 leucine-rich repeat transmembrane protein
kinase, putative
Length = 669
Score = 27.9 bits (59), Expect = 5.4
Identities = 10/33 (30%), Positives = 24/33 (72%)
Frame = -1
Query: 563 ESSLLYTSHVKFNFCKDEGIRCIKHGRVVGLLV 465
++ LLY+ ++++C+ G++C + GR+V L++
Sbjct: 48 DNKLLYSLTERYDYCQWRGVKCAQ-GRIVRLVL 79
>At5g01140.1 68418.m00018 hypothetical protein contains Pfam profile
PF05056: Protein of unknown function (DUF674)
Length = 473
Score = 27.9 bits (59), Expect = 5.4
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = +3
Query: 126 NKICLTMAGTIFRLLLTNTTSAHKNITTIRSINTGTV 236
NK+ L AGTI RLL + S I +R++ T V
Sbjct: 21 NKVVLAEAGTIVRLLAKHRKSDPVTIGCLRNLYTSVV 57
>At3g54660.1 68416.m06048 gluthatione reductase, chloroplast nearly
identical to SP|P42770 Glutathione reductase,
chloroplast precursor (EC 1.8.1.7) (GR) (GRASE)
{Arabidopsis thaliana}; identical to cDNA glutathione
reductase GI:451197
Length = 565
Score = 27.9 bits (59), Expect = 5.4
Identities = 15/40 (37%), Positives = 18/40 (45%)
Frame = -1
Query: 263 SCTFSGCVEYRSCVYGSNSCNIFVG*SGIGQKQTENSPSH 144
+C GCV + VY S + F G G K E PSH
Sbjct: 134 TCVLRGCVPKKLLVYASKYSHEFEDSHGFGWKY-ETEPSH 172
>At5g22355.1 68418.m02608 DC1 domain-containing protein contains
Pfam PF03107: DC1 domain; similar to ULI3 [Arabidopsis
thaliana] GI:17225050
Length = 664
Score = 27.1 bits (57), Expect = 9.5
Identities = 19/66 (28%), Positives = 27/66 (40%), Gaps = 2/66 (3%)
Frame = -1
Query: 398 DGYRFVLITEMLVHHCFNYLSPSLI-VRNETNFCDFNLNFFCTCLSSCTFSGCVEY-RSC 225
DG+R L E HC + + + CDF+L+ CT L C + S
Sbjct: 496 DGHRHPLYYEHKKDHCCDACYKEIDGYLLSCDTCDFDLDLHCTDLPKTVKHSCDNHPLSL 555
Query: 224 VYGSNS 207
YG N+
Sbjct: 556 CYGENA 561
>At3g08730.1 68416.m01015 serine/threonine protein kinase (PK1)
(PK6) identical to serine/threonine-protein kinase
AtPK1/AtPK6 (ribosomal-protein S6 kinase ATPK6)
[Arabidopsis thaliana] SWISS-PROT:P42818
Length = 465
Score = 27.1 bits (57), Expect = 9.5
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = -1
Query: 452 ILSRVDHPFVILHRIHXVDGYRFVLITEML 363
IL+++DHPF++ + YR L+ + +
Sbjct: 185 ILTKIDHPFIVQLKYSFQTKYRLYLVLDFI 214
>At3g08720.2 68416.m01014 serine/threonine protein kinase (PK19)
identical to serine/threonine-protein kinase AtPK19
(Ribosomal-protein S6 kinase homolog) [Arabidopsis
thaliana] SWISS-PROT:Q39030
Length = 471
Score = 27.1 bits (57), Expect = 9.5
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = -1
Query: 452 ILSRVDHPFVILHRIHXVDGYRFVLITEML 363
IL+++DHPF++ + YR L+ + +
Sbjct: 191 ILTKIDHPFIVQLKYSFQTKYRLYLVLDFI 220
>At3g08720.1 68416.m01013 serine/threonine protein kinase (PK19)
identical to serine/threonine-protein kinase AtPK19
(Ribosomal-protein S6 kinase homolog) [Arabidopsis
thaliana] SWISS-PROT:Q39030
Length = 471
Score = 27.1 bits (57), Expect = 9.5
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = -1
Query: 452 ILSRVDHPFVILHRIHXVDGYRFVLITEML 363
IL+++DHPF++ + YR L+ + +
Sbjct: 191 ILTKIDHPFIVQLKYSFQTKYRLYLVLDFI 220
>At1g74180.1 68414.m08591 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Hcr2-0B [Lycopersicon
esculentum] gi|3894387|gb|AAC78593
Length = 951
Score = 27.1 bits (57), Expect = 9.5
Identities = 11/30 (36%), Positives = 20/30 (66%), Gaps = 1/30 (3%)
Frame = -1
Query: 548 YTSHVKFNFCKDEGIRCIK-HGRVVGLLVG 462
+T+ K N C+ EG++C + GR++ L +G
Sbjct: 58 WTNDTKSNCCRWEGLKCNQTSGRIIELSIG 87
>At1g23970.2 68414.m03027 expressed protein contains Pfam profile
PF04776: Protein of unknown function (DUF626)
Length = 378
Score = 27.1 bits (57), Expect = 9.5
Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 8/60 (13%)
Frame = -1
Query: 491 HGRVVGLLVGFDCILS-RVDHPFVI-------LHRIHXVDGYRFVLITEMLVHHCFNYLS 336
HGRV+GL+ ++C L R P ++ LHR + + G F L M + NY+S
Sbjct: 43 HGRVMGLIT-YNCQLCYRYPFPVLVKLYAKFGLHRYNLIKGTSFELAALMKFNMLQNYMS 101
>At1g23970.1 68414.m03026 expressed protein contains Pfam profile
PF04776: Protein of unknown function (DUF626)
Length = 373
Score = 27.1 bits (57), Expect = 9.5
Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 8/60 (13%)
Frame = -1
Query: 491 HGRVVGLLVGFDCILS-RVDHPFVI-------LHRIHXVDGYRFVLITEMLVHHCFNYLS 336
HGRV+GL+ ++C L R P ++ LHR + + G F L M + NY+S
Sbjct: 43 HGRVMGLIT-YNCQLCYRYPFPVLVKLYAKFGLHRYNLIKGTSFELAALMKFNMLQNYMS 101
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,251,511
Number of Sequences: 28952
Number of extensions: 255064
Number of successful extensions: 611
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 597
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 611
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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