BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_M11 (653 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1B3.07c |vps28||ESCRT I complex subunit Vps28|Schizosaccharo... 62 1e-15 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 29 0.59 SPBC29A10.06c |||conserved fungal protein|Schizosaccharomyces po... 26 4.1 SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 7.2 SPCPB1C11.02 |||amino acid permease, unknown 16|Schizosaccharomy... 25 7.2 SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 25 9.5 SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |S... 25 9.5 SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ... 25 9.5 SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|ch... 25 9.5 >SPAC1B3.07c |vps28||ESCRT I complex subunit Vps28|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 62.5 bits (145), Expect(2) = 1e-15 Identities = 29/107 (27%), Positives = 59/107 (55%), Gaps = 1/107 (0%) Frame = +3 Query: 213 NARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFKQVQADE 392 + + + ++++ LY+++ L+ L KA+ +D V ++ + C L+ Q++ F + + Sbjct: 21 HTKNQQVREDLSILYSILVALEQLEKAFTKDAVSTSDFNSTCELLIQQWESCFSDERVTQ 80 Query: 393 -FPNIEAFVAKYRLXCPAALERIXENKPNLIXDDKGNTNKYIAEIVS 530 F + E F +KYRL CP A++RI E I D++ +N + +S Sbjct: 81 AFGSFEDFCSKYRLQCPRAIKRIQEG----ISDERSQSNSTFSNAIS 123 Score = 35.9 bits (79), Expect(2) = 1e-15 Identities = 19/42 (45%), Positives = 26/42 (61%) Frame = +3 Query: 507 KYIAEIVSLFITLMDKLRLXFRAMDMIQPELRDLRDTMDRLS 632 K IA +V FIT +D +RL F A D + P L +L +MD L+ Sbjct: 155 KSIAGLVQNFITTLDAIRLNFIAKDQLHPLLSELIVSMDDLT 196 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 29.1 bits (62), Expect = 0.59 Identities = 16/46 (34%), Positives = 21/46 (45%) Frame = -3 Query: 426 CI*QQKLQYWETHQPVLV*KPLCTARGDDCMPPCIPVLAHNHACMP 289 C Q+K ++ + P P RG DC PC P+L H C P Sbjct: 261 CGKQEKPEFVKNLVPHSCGDPCGKTRGQDCEHPC-PLLCHPGPCPP 305 >SPBC29A10.06c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 295 Score = 26.2 bits (55), Expect = 4.1 Identities = 19/50 (38%), Positives = 27/50 (54%) Frame = -2 Query: 535 NNDTISAMYLLVFPLSSLMRFGLFSXIRSKAAGQXNLYLATKASILGNSS 386 +NDT S ++ PLS LFS +RSK + LYL T ++L S+ Sbjct: 217 SNDTQSCKLIVSLPLSQEECEVLFSLLRSK---KEPLYLNTLLALLVESN 263 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = -1 Query: 368 SHFVLHEETTACRRVFLCSHTITHVCLLQVLKGTDDSIEL 249 S+F L++E CRR+ C+H C+ Q L + +S L Sbjct: 530 SNFELNDE---CRRLKQCNHFFHRECIDQWLTSSQNSCPL 566 >SPCPB1C11.02 |||amino acid permease, unknown 16|Schizosaccharomyces pombe|chr 3|||Manual Length = 505 Score = 25.4 bits (53), Expect = 7.2 Identities = 16/52 (30%), Positives = 23/52 (44%) Frame = -2 Query: 526 TISAMYLLVFPLSSLMRFGLFSXIRSKAAGQXNLYLATKASILGNSSACTCL 371 ++SA + + ++ GLF I S A L S+LG S CT L Sbjct: 17 SLSASQIQMLAFGGIIGTGLFLGIGSSLAESGPASLLISFSVLGVSVYCTML 68 >SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1689 Score = 25.0 bits (52), Expect = 9.5 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = +1 Query: 463 RTNRISSXMTKGTLINTLQKLYRYL 537 ++N ISS + LINT +K+YR++ Sbjct: 573 QSNLISSPYSYSRLINTNKKVYRHV 597 >SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |Schizosaccharomyces pombe|chr 1|||Manual Length = 672 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/34 (38%), Positives = 16/34 (47%) Frame = +3 Query: 225 RXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEY 326 R H N+ LY VVCT + A M C + Y Sbjct: 79 RLLHPNITRLYEVVCTEDRIYLA-MEYCPNGELY 111 >SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar membrane transporter Hmt1|Schizosaccharomyces pombe|chr 3|||Manual Length = 830 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/27 (37%), Positives = 15/27 (55%) Frame = +1 Query: 73 YYFIIYKTRFLAVCFII*YCDCYKSVK 153 Y+FI + F + I+ C CY +VK Sbjct: 398 YFFIKFDIYFTLIVLIMTLCYCYVTVK 424 >SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|chr 3|||Manual Length = 862 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = +1 Query: 559 VXSSVRWT*FSLNFVTFATPWTGS 630 V S+ + FS+ + F PWTGS Sbjct: 192 VVVSLAYVSFSIIYALFLNPWTGS 215 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,384,275 Number of Sequences: 5004 Number of extensions: 45689 Number of successful extensions: 138 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 125 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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