BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_M11 (653 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g05000.2 68417.m00731 vacuolar protein sorting-associated pro... 116 1e-26 At4g05000.1 68417.m00730 vacuolar protein sorting-associated pro... 116 1e-26 At4g21560.3 68417.m03116 vacuolar protein sorting-associated pro... 111 5e-25 At4g21560.2 68417.m03115 vacuolar protein sorting-associated pro... 111 5e-25 At4g21560.1 68417.m03114 vacuolar protein sorting-associated pro... 111 5e-25 At5g41140.1 68418.m05001 expressed protein 28 6.2 At2g22610.1 68415.m02680 kinesin motor protein-related 28 6.2 At5g03435.1 68418.m00297 C2 domain-containing protein contains P... 27 8.2 >At4g05000.2 68417.m00731 vacuolar protein sorting-associated protein 28 family protein / VPS28 family protein contains similarity to Swiss-Prot:Q02767 vacuolar protein sorting-associated protein VPS28 [Saccharomyces cerevisiae] Length = 210 Score = 116 bits (279), Expect = 1e-26 Identities = 61/160 (38%), Positives = 92/160 (57%), Gaps = 7/160 (4%) Frame = +3 Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374 E+KL+ + RER ++N AEL+A++ + L KAY+RD + EY + C +L+V +K Sbjct: 3 EVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLSA 62 Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533 ++ D PNIE F Y++ CPAAL R + P + N+ +AE V Sbjct: 63 TLK-DTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121 Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653 FIT MD L+L A+D + P L DL ++++LS+LP DFE Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 161 >At4g05000.1 68417.m00730 vacuolar protein sorting-associated protein 28 family protein / VPS28 family protein contains similarity to Swiss-Prot:Q02767 vacuolar protein sorting-associated protein VPS28 [Saccharomyces cerevisiae] Length = 210 Score = 116 bits (279), Expect = 1e-26 Identities = 61/160 (38%), Positives = 92/160 (57%), Gaps = 7/160 (4%) Frame = +3 Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374 E+KL+ + RER ++N AEL+A++ + L KAY+RD + EY + C +L+V +K Sbjct: 3 EVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLSA 62 Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533 ++ D PNIE F Y++ CPAAL R + P + N+ +AE V Sbjct: 63 TLK-DTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121 Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653 FIT MD L+L A+D + P L DL ++++LS+LP DFE Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 161 >At4g21560.3 68417.m03116 vacuolar protein sorting-associated protein 28 family protein / VPS28 family protein contains similarity to Swiss-Prot:Q02767 vacuolar protein sorting-associated protein VPS28 [Saccharomyces cerevisiae] Length = 209 Score = 111 bits (266), Expect = 5e-25 Identities = 59/160 (36%), Positives = 90/160 (56%), Gaps = 7/160 (4%) Frame = +3 Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374 E+KL+ + RER ++N AELYA++ + L KAY+RD + EY C +L+V +K Sbjct: 2 EVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLSA 61 Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533 ++ D PNIE F Y++ C AA+ R + P + ++ +AE V Sbjct: 62 SLK-DMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120 Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653 FIT MD L+L A+D + P L DL ++++LS+LP DFE Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 160 >At4g21560.2 68417.m03115 vacuolar protein sorting-associated protein 28 family protein / VPS28 family protein contains similarity to Swiss-Prot:Q02767 vacuolar protein sorting-associated protein VPS28 [Saccharomyces cerevisiae] Length = 209 Score = 111 bits (266), Expect = 5e-25 Identities = 59/160 (36%), Positives = 90/160 (56%), Gaps = 7/160 (4%) Frame = +3 Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374 E+KL+ + RER ++N AELYA++ + L KAY+RD + EY C +L+V +K Sbjct: 2 EVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLSA 61 Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533 ++ D PNIE F Y++ C AA+ R + P + ++ +AE V Sbjct: 62 SLK-DMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120 Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653 FIT MD L+L A+D + P L DL ++++LS+LP DFE Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 160 >At4g21560.1 68417.m03114 vacuolar protein sorting-associated protein 28 family protein / VPS28 family protein contains similarity to Swiss-Prot:Q02767 vacuolar protein sorting-associated protein VPS28 [Saccharomyces cerevisiae] Length = 209 Score = 111 bits (266), Expect = 5e-25 Identities = 59/160 (36%), Positives = 90/160 (56%), Gaps = 7/160 (4%) Frame = +3 Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374 E+KL+ + RER ++N AELYA++ + L KAY+RD + EY C +L+V +K Sbjct: 2 EVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLSA 61 Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533 ++ D PNIE F Y++ C AA+ R + P + ++ +AE V Sbjct: 62 SLK-DMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120 Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653 FIT MD L+L A+D + P L DL ++++LS+LP DFE Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 160 >At5g41140.1 68418.m05001 expressed protein Length = 983 Score = 27.9 bits (59), Expect = 6.2 Identities = 28/99 (28%), Positives = 44/99 (44%), Gaps = 5/99 (5%) Frame = +3 Query: 183 ELYEEIKLYKNARERXKHDNMAELYAVVCTLQH-LXKAYMRDCVRAQEYTAACSRLLVQY 359 +LY EI++YK R K D E+ +L + + K D E + +L +QY Sbjct: 483 DLYNEIEIYK----RDKED--LEIQVEQLSLDYEILKQENHDISYKLEQSQVQEQLKMQY 536 Query: 360 KVAFKQVQADEFPN----IEAFVAKYRLXCPAALERIXE 464 + + V +E N +EA + K C +L RI E Sbjct: 537 ECSSSLVNVNELENHVESLEAKLKKQYKECSESLYRIKE 575 >At2g22610.1 68415.m02680 kinesin motor protein-related Length = 1093 Score = 27.9 bits (59), Expect = 6.2 Identities = 18/57 (31%), Positives = 28/57 (49%), Gaps = 3/57 (5%) Frame = +3 Query: 351 VQYKVAFKQVQADEFPNIEAFVAKYRLXCPA---ALERIXENKPNLIXDDKGNTNKY 512 V+ A KQV E ++A V K R + +++++ EN NL +KG N Y Sbjct: 749 VELGPARKQVDTGEIQKLKAMVEKARQESRSKDESIKKMEENIQNLEGKNKGRDNSY 805 >At5g03435.1 68418.m00297 C2 domain-containing protein contains Pfam profile PF00168: C2 domain Length = 745 Score = 27.5 bits (58), Expect = 8.2 Identities = 12/38 (31%), Positives = 18/38 (47%) Frame = -3 Query: 195 LHTIPDVYLASLLFLHRFITITISNNETNSEKPRFINN 82 L +P + L L LH+F+T N N P +N+ Sbjct: 577 LVVVPCIVLVGLSGLHKFLTRKFWNKRENPRSPLIVND 614 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,159,121 Number of Sequences: 28952 Number of extensions: 230611 Number of successful extensions: 625 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 595 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 615 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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