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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_M11
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g05000.2 68417.m00731 vacuolar protein sorting-associated pro...   116   1e-26
At4g05000.1 68417.m00730 vacuolar protein sorting-associated pro...   116   1e-26
At4g21560.3 68417.m03116 vacuolar protein sorting-associated pro...   111   5e-25
At4g21560.2 68417.m03115 vacuolar protein sorting-associated pro...   111   5e-25
At4g21560.1 68417.m03114 vacuolar protein sorting-associated pro...   111   5e-25
At5g41140.1 68418.m05001 expressed protein                             28   6.2  
At2g22610.1 68415.m02680 kinesin motor protein-related                 28   6.2  
At5g03435.1 68418.m00297 C2 domain-containing protein contains P...    27   8.2  

>At4g05000.2 68417.m00731 vacuolar protein sorting-associated
           protein 28 family protein / VPS28 family protein
           contains similarity to Swiss-Prot:Q02767 vacuolar
           protein sorting-associated protein VPS28 [Saccharomyces
           cerevisiae]
          Length = 210

 Score =  116 bits (279), Expect = 1e-26
 Identities = 61/160 (38%), Positives = 92/160 (57%), Gaps = 7/160 (4%)
 Frame = +3

Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374
           E+KL+ + RER  ++N AEL+A++   + L KAY+RD +   EY + C +L+V +K    
Sbjct: 3   EVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLSA 62

Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533
            ++ D  PNIE F   Y++ CPAAL R +    P  +           N+   +AE V  
Sbjct: 63  TLK-DTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121

Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653
           FIT MD L+L   A+D + P L DL  ++++LS+LP DFE
Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 161


>At4g05000.1 68417.m00730 vacuolar protein sorting-associated
           protein 28 family protein / VPS28 family protein
           contains similarity to Swiss-Prot:Q02767 vacuolar
           protein sorting-associated protein VPS28 [Saccharomyces
           cerevisiae]
          Length = 210

 Score =  116 bits (279), Expect = 1e-26
 Identities = 61/160 (38%), Positives = 92/160 (57%), Gaps = 7/160 (4%)
 Frame = +3

Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374
           E+KL+ + RER  ++N AEL+A++   + L KAY+RD +   EY + C +L+V +K    
Sbjct: 3   EVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLSA 62

Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533
            ++ D  PNIE F   Y++ CPAAL R +    P  +           N+   +AE V  
Sbjct: 63  TLK-DTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121

Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653
           FIT MD L+L   A+D + P L DL  ++++LS+LP DFE
Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 161


>At4g21560.3 68417.m03116 vacuolar protein sorting-associated
           protein 28 family protein / VPS28 family protein
           contains similarity to Swiss-Prot:Q02767 vacuolar
           protein sorting-associated protein VPS28 [Saccharomyces
           cerevisiae]
          Length = 209

 Score =  111 bits (266), Expect = 5e-25
 Identities = 59/160 (36%), Positives = 90/160 (56%), Gaps = 7/160 (4%)
 Frame = +3

Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374
           E+KL+ + RER  ++N AELYA++   + L KAY+RD +   EY   C +L+V +K    
Sbjct: 2   EVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLSA 61

Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533
            ++ D  PNIE F   Y++ C AA+ R +    P  +           ++   +AE V  
Sbjct: 62  SLK-DMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653
           FIT MD L+L   A+D + P L DL  ++++LS+LP DFE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 160


>At4g21560.2 68417.m03115 vacuolar protein sorting-associated
           protein 28 family protein / VPS28 family protein
           contains similarity to Swiss-Prot:Q02767 vacuolar
           protein sorting-associated protein VPS28 [Saccharomyces
           cerevisiae]
          Length = 209

 Score =  111 bits (266), Expect = 5e-25
 Identities = 59/160 (36%), Positives = 90/160 (56%), Gaps = 7/160 (4%)
 Frame = +3

Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374
           E+KL+ + RER  ++N AELYA++   + L KAY+RD +   EY   C +L+V +K    
Sbjct: 2   EVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLSA 61

Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533
            ++ D  PNIE F   Y++ C AA+ R +    P  +           ++   +AE V  
Sbjct: 62  SLK-DMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653
           FIT MD L+L   A+D + P L DL  ++++LS+LP DFE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 160


>At4g21560.1 68417.m03114 vacuolar protein sorting-associated
           protein 28 family protein / VPS28 family protein
           contains similarity to Swiss-Prot:Q02767 vacuolar
           protein sorting-associated protein VPS28 [Saccharomyces
           cerevisiae]
          Length = 209

 Score =  111 bits (266), Expect = 5e-25
 Identities = 59/160 (36%), Positives = 90/160 (56%), Gaps = 7/160 (4%)
 Frame = +3

Query: 195 EIKLYKNARERXKHDNMAELYAVVCTLQHLXKAYMRDCVRAQEYTAACSRLLVQYKVAFK 374
           E+KL+ + RER  ++N AELYA++   + L KAY+RD +   EY   C +L+V +K    
Sbjct: 2   EVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLSA 61

Query: 375 QVQADEFPNIEAFVAKYRLXCPAALER-IXENKPNLIXD------DKGNTNKYIAEIVSL 533
            ++ D  PNIE F   Y++ C AA+ R +    P  +           ++   +AE V  
Sbjct: 62  SLK-DMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 534 FITLMDKLRLXFRAMDMIQPELRDLRDTMDRLSMLPDDFE 653
           FIT MD L+L   A+D + P L DL  ++++LS+LP DFE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFE 160


>At5g41140.1 68418.m05001 expressed protein
          Length = 983

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 28/99 (28%), Positives = 44/99 (44%), Gaps = 5/99 (5%)
 Frame = +3

Query: 183 ELYEEIKLYKNARERXKHDNMAELYAVVCTLQH-LXKAYMRDCVRAQEYTAACSRLLVQY 359
           +LY EI++YK    R K D   E+     +L + + K    D     E +    +L +QY
Sbjct: 483 DLYNEIEIYK----RDKED--LEIQVEQLSLDYEILKQENHDISYKLEQSQVQEQLKMQY 536

Query: 360 KVAFKQVQADEFPN----IEAFVAKYRLXCPAALERIXE 464
           + +   V  +E  N    +EA + K    C  +L RI E
Sbjct: 537 ECSSSLVNVNELENHVESLEAKLKKQYKECSESLYRIKE 575


>At2g22610.1 68415.m02680 kinesin motor protein-related 
          Length = 1093

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 18/57 (31%), Positives = 28/57 (49%), Gaps = 3/57 (5%)
 Frame = +3

Query: 351 VQYKVAFKQVQADEFPNIEAFVAKYRLXCPA---ALERIXENKPNLIXDDKGNTNKY 512
           V+   A KQV   E   ++A V K R    +   +++++ EN  NL   +KG  N Y
Sbjct: 749 VELGPARKQVDTGEIQKLKAMVEKARQESRSKDESIKKMEENIQNLEGKNKGRDNSY 805


>At5g03435.1 68418.m00297 C2 domain-containing protein contains Pfam
           profile PF00168: C2 domain
          Length = 745

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 12/38 (31%), Positives = 18/38 (47%)
 Frame = -3

Query: 195 LHTIPDVYLASLLFLHRFITITISNNETNSEKPRFINN 82
           L  +P + L  L  LH+F+T    N   N   P  +N+
Sbjct: 577 LVVVPCIVLVGLSGLHKFLTRKFWNKRENPRSPLIVND 614


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,159,121
Number of Sequences: 28952
Number of extensions: 230611
Number of successful extensions: 625
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 595
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 615
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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