BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_M09 (642 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 34 0.070 At1g07550.1 68414.m00808 leucine-rich repeat protein kinase, put... 33 0.16 At4g36830.1 68417.m05223 GNS1/SUR4 membrane family protein weak ... 31 0.65 At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 31 0.86 At5g05140.1 68418.m00545 transcription elongation factor-related... 30 1.1 At1g77220.1 68414.m08994 expressed protein contains Pfam profile... 29 2.0 At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 29 3.5 At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 29 3.5 At4g23750.2 68417.m03417 AP2 domain-containing transcription fac... 29 3.5 At4g23750.1 68417.m03416 AP2 domain-containing transcription fac... 29 3.5 At2g28470.1 68415.m03460 beta-galactosidase, putative / lactase,... 29 3.5 At1g64160.1 68414.m07268 disease resistance-responsive family pr... 29 3.5 At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 28 4.6 >At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1 [Mus musculus] GI:3228668; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 307 Score = 34.3 bits (75), Expect = 0.070 Identities = 43/161 (26%), Positives = 68/161 (42%), Gaps = 4/161 (2%) Frame = +1 Query: 100 LFVSLTKTS---FQKSTPQDSQYVAAAVEYIVSGDVDQNIRNYVRYIEEAAKQNADIIVF 270 LF +T +S +T + AA + D+ N R ++EAA A +I F Sbjct: 15 LFTRITLSSQIPLTMATTVNKTVRVAAAQMTSVNDLMTNFATCSRLVQEAALAGAKLICF 74 Query: 271 PELTLTNRSTSFVAPLHGILKQYPIPALHPNLYDNIMVSISAAARTNQIYVVVNG-RELM 447 PE + SFV G + P P +M + AR + I++ + G +E Sbjct: 75 PE------NFSFVGDKEGESVKIAEPLDGP-----VMERYCSLARDSNIWLSLGGFQERF 123 Query: 448 DCTKNDTGEPCPELKEYIFNTNVVFDRNGAVIDRYRKINLF 570 D T ++ NT+VV D G + D Y+K++LF Sbjct: 124 DDT-------------HLCNTHVVIDDAGMIRDTYQKMHLF 151 >At1g07550.1 68414.m00808 leucine-rich repeat protein kinase, putative similar to light repressible receptor protein kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376; contains leucine rich repeat (LRR) domains, Pfam:PF00560; contains protein kinase domain, Pfam:PF00069 Length = 864 Score = 33.1 bits (72), Expect = 0.16 Identities = 26/107 (24%), Positives = 47/107 (43%), Gaps = 3/107 (2%) Frame = +1 Query: 214 NYVRYIEEAAKQNADIIVFPELTLTNRSTSFVAPLHGILKQYPI---PALHPNLYDNIMV 384 NY+RY ++ + ++ PE T N S + + G + A+ N D + + Sbjct: 204 NYIRYPQDVHDRIWVPLILPEWTHINTSHHVIDSIDGYDPPQDVLRTGAMPANASDPMTI 263 Query: 385 SISAAARTNQIYVVVNGRELMDCTKNDTGEPCPELKEYIFNTNVVFD 525 + + T+Q+Y + E+M+ N+T E E + N V FD Sbjct: 264 TWNLKTATDQVYGYIYIAEIMEVQANETRE-----FEVVVNNKVHFD 305 >At4g36830.1 68417.m05223 GNS1/SUR4 membrane family protein weak similarity to long chain polyunsaturated fatty acid elongation enzyme [Isochrysis galbana] GI:17226123; contains Pfam profile PF01151: GNS1/SUR4 family Length = 289 Score = 31.1 bits (67), Expect = 0.65 Identities = 17/41 (41%), Positives = 26/41 (63%), Gaps = 2/41 (4%) Frame = -1 Query: 555 AIPINNRSVPIKHNVCIKDILLQL--RTRFAGVILSAVHEL 439 A+ +NRSVP+ H I +L+ + T FAG++LSA E+ Sbjct: 57 AVRRSNRSVPLGHIPEIHSLLMSILSATIFAGILLSAAAEI 97 >At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961 Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana} Length = 346 Score = 30.7 bits (66), Expect = 0.86 Identities = 16/52 (30%), Positives = 23/52 (44%) Frame = +1 Query: 121 TSFQKSTPQDSQYVAAAVEYIVSGDVDQNIRNYVRYIEEAAKQNADIIVFPE 276 T F P + V V D I +YI EAA + A++++FPE Sbjct: 14 TPFNGVAPSTTVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPE 65 >At5g05140.1 68418.m00545 transcription elongation factor-related low similarity to transcription elongation factor TFIIS.h [Mus musculus] GI:3288547, elongin A [Mus musculus] GI:6009624 Length = 436 Score = 30.3 bits (65), Expect = 1.1 Identities = 20/59 (33%), Positives = 28/59 (47%) Frame = -1 Query: 579 TFPEKIDLAIPINNRSVPIKHNVCIKDILLQLRTRFAGVILSAVHELSAIDHNVDLISS 403 TF E L+ I SV K IK+ILL V+L ++ L + NVD++ S Sbjct: 120 TFDEAEALSDEIEEFSVVSKEVARIKEILLNKEDEPNSVLLDSLRHLKLMSLNVDILKS 178 >At1g77220.1 68414.m08994 expressed protein contains Pfam profile PF03619: Domain of unknown function Length = 484 Score = 29.5 bits (63), Expect = 2.0 Identities = 17/49 (34%), Positives = 25/49 (51%) Frame = +1 Query: 268 FPELTLTNRSTSFVAPLHGILKQYPIPALHPNLYDNIMVSISAAARTNQ 414 F + T + S+V PL+ K++ +H NLYD VS S TN+ Sbjct: 423 FEQQKKTTKDDSYVIPLNQWAKEFS--DVHENLYDGGSVSDSGLGSTNR 469 >At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein similar to Nit protein 2 [Homo sapiens] GI:9367116; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 294 Score = 28.7 bits (61), Expect = 3.5 Identities = 12/32 (37%), Positives = 21/32 (65%) Frame = +1 Query: 184 VSGDVDQNIRNYVRYIEEAAKQNADIIVFPEL 279 V+ D +NI + + IEEAA + A +++ PE+ Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEI 128 >At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein similar to Nit protein 2 [Homo sapiens] GI:9367116; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 369 Score = 28.7 bits (61), Expect = 3.5 Identities = 12/32 (37%), Positives = 21/32 (65%) Frame = +1 Query: 184 VSGDVDQNIRNYVRYIEEAAKQNADIIVFPEL 279 V+ D +NI + + IEEAA + A +++ PE+ Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEI 128 >At4g23750.2 68417.m03417 AP2 domain-containing transcription factor, putative DNA-binding protein Pti6 - Lycopersicon esculentum,PID:g2213785 Length = 343 Score = 28.7 bits (61), Expect = 3.5 Identities = 16/45 (35%), Positives = 24/45 (53%) Frame = +1 Query: 499 IFNTNVVFDRNGAVIDRYRKINLFGESSHTPALTPDLGYFETDFG 633 +F+ + VFD +V+ Y +LFGE T + D+ F DFG Sbjct: 273 LFSDDDVFDFRSSVVPDYLGGDLFGEDLFTADMCTDMN-FGFDFG 316 >At4g23750.1 68417.m03416 AP2 domain-containing transcription factor, putative DNA-binding protein Pti6 - Lycopersicon esculentum,PID:g2213785 Length = 343 Score = 28.7 bits (61), Expect = 3.5 Identities = 16/45 (35%), Positives = 24/45 (53%) Frame = +1 Query: 499 IFNTNVVFDRNGAVIDRYRKINLFGESSHTPALTPDLGYFETDFG 633 +F+ + VFD +V+ Y +LFGE T + D+ F DFG Sbjct: 273 LFSDDDVFDFRSSVVPDYLGGDLFGEDLFTADMCTDMN-FGFDFG 316 >At2g28470.1 68415.m03460 beta-galactosidase, putative / lactase, putative similar to Beta-galactosidase precursor SP:P48980 from [Lycopersicon esculentum] Length = 852 Score = 28.7 bits (61), Expect = 3.5 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = +1 Query: 511 NVVFDRNGAVIDRYRKINLFGESSHTPALTPDL 609 NV +D VID RK+ + G S H P TP++ Sbjct: 31 NVTYDHRALVIDGKRKVLISG-SIHYPRSTPEM 62 >At1g64160.1 68414.m07268 disease resistance-responsive family protein / dirigent family protein similar to dirigent protein GB:AAF25365 GI:6694709 from [Thuja plicata]; similar to pathogenesis-related protein [Pisum sativum] gi:4585273 gb:AAD25355 Length = 182 Score = 28.7 bits (61), Expect = 3.5 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = +1 Query: 70 GIMKGFIFCLLFVSLTKTSFQKSTPQDSQ 156 G MK F+F +F+ LTKT P SQ Sbjct: 3 GQMKSFLFLFVFLVLTKTVISARKPSKSQ 31 >At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-alanine synthase, putative similar to beta-alanine synthase [Dictyostelium discoideum] GI:14334061; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 408 Score = 28.3 bits (60), Expect = 4.6 Identities = 19/54 (35%), Positives = 31/54 (57%), Gaps = 7/54 (12%) Frame = +1 Query: 493 EYIFNTNVVFDRNGAVIDRYRKINL-----FGESSHTPALTPDLGY--FETDFG 633 E ++NT V+ NG +I ++RK ++ F ES++ + D G+ FET FG Sbjct: 191 EVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTY--YMEGDTGHPVFETVFG 242 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,951,590 Number of Sequences: 28952 Number of extensions: 252373 Number of successful extensions: 697 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 683 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 696 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1324661040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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