Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_M08
(651 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g06000.1 68418.m00665 eukaryotic translation initiation facto... 80 1e-15
At3g11400.1 68416.m01390 eukaryotic translation initiation facto... 72 3e-13
At1g43560.1 68414.m05000 thioredoxin family protein contains Pfa... 29 2.7
At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putativ... 29 3.5
At2g30960.1 68415.m03776 expressed protein 29 3.5
At1g52650.1 68414.m05945 F-box family protein contains F-box dom... 28 6.2
At5g57170.1 68418.m07141 macrophage migration inhibitory factor ... 27 8.2
At2g36350.1 68415.m04461 protein kinase, putative similar to pro... 27 8.2
At1g76760.1 68414.m08933 thioredoxin family protein similar to t... 27 8.2
At1g67370.1 68414.m07669 meiotic asynaptic mutant 1 (ASY1) ident... 27 8.2
At1g57820.2 68414.m06561 zinc finger (C3HC4-type RING finger) fa... 27 8.2
At1g57820.1 68414.m06560 zinc finger (C3HC4-type RING finger) fa... 27 8.2
>At5g06000.1 68418.m00665 eukaryotic translation initiation factor
3G, putative / eIF3g, putative similar to eukaryotic
translation initiation factor 3g [Arabidopsis thaliana]
GI:12407751; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 276
Score = 80.2 bits (189), Expect = 1e-15
Identities = 43/119 (36%), Positives = 68/119 (57%), Gaps = 5/119 (4%)
Frame = +3
Query: 201 ENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATT 380
+NG+K V EYK++ ++KKVKI T +++KR ++K +R++W+KFGD+A ++ + T
Sbjct: 6 QNGVKKVIEYKFNEEDKKVKITTTTRVQKRALTKQAVERRSWNKFGDAAHEE-SSSYLTM 64
Query: 381 NVAEDVFMQFIT---SKEESQRPDDGELDGLKPPSSNVIFKCRTCQ--GDHLTLYCPFK 542
ED+ ++ I S E + L P + V+ CR CQ GDH T CP K
Sbjct: 65 RSTEDIILERIRAPGSNAEQSTVSGDSMSQLGKPGA-VLMVCRLCQKKGDHWTSRCPQK 122
>At3g11400.1 68416.m01390 eukaryotic translation initiation factor
3G / eIF3g nearly identical to eukaryotic translation
initiation factor 3g [Arabidopsis thaliana] GI:12407751
Length = 294
Score = 72.1 bits (169), Expect = 3e-13
Identities = 52/150 (34%), Positives = 80/150 (53%), Gaps = 12/150 (8%)
Frame = +3
Query: 129 EFQASWADEVEIDQGVLPPPSEVV---ENGLKIVTEYKYDNDNKKVKI---VRTYKIEKR 290
+F+ DE E D L PP +V+ ENGLK EYK++++ KVKI R K+
Sbjct: 11 KFRWGEMDEDE-DLDFLLPPKQVIGPDENGLKTTIEYKFNDEENKVKITTRTRVRKLASA 69
Query: 291 VVSKSIAKRKTWSKFGDSASDKPGPNPATTNVAEDVFMQFI----TSKEESQRPDDGELD 458
++K +R+ W KFGD+A+++ G + T E++ ++ T +ES+ DG L
Sbjct: 70 RLNKRAMERRNWPKFGDAANEEAGSH-LTMVSTEEILLERPRAPGTKADESKATGDG-LS 127
Query: 459 GLKPPSSNVIFKCRTC--QGDHLTLYCPFK 542
L + V+ CR C +GDH T CP+K
Sbjct: 128 QLGKGGA-VLMVCRICHKKGDHWTSKCPYK 156
>At1g43560.1 68414.m05000 thioredoxin family protein contains Pfam
profile: PF00085 Thioredoxin; similar to thioredoxin
GI:142153 from [Synechococcus PCC6301]
Length = 167
Score = 29.1 bits (62), Expect = 2.7
Identities = 20/75 (26%), Positives = 32/75 (42%)
Frame = +3
Query: 111 DMPVAEEFQASWADEVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIEKR 290
D PV +F A+W ++ ++P +EV E I+ K D + K + Y+IE
Sbjct: 76 DKPVLVDFYATWCGPCQL---MVPILNEVSETLKDIIAVVKIDTE-KYPSLANKYQIEAL 131
Query: 291 VVSKSIAKRKTWSKF 335
K W +F
Sbjct: 132 PTFILFKDGKLWDRF 146
>At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative identical to SP|O22682 Probable
cysteine synthase, chloroplast precursor {Arabidopsis
thaliana}, similar to SP|P31300 Cysteine synthase,
chloroplast precursor {Capsicum annuum}
Length = 404
Score = 28.7 bits (61), Expect = 3.5
Identities = 17/56 (30%), Positives = 24/56 (42%)
Frame = +3
Query: 228 YKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATTNVAED 395
+ + +D+ VRT + VV K T +K KP P P T VAE+
Sbjct: 34 FSFHHDSSSSLAVRT-PVSSFVVGAISGKSSTGTKSKSKTKRKPPPPPPVTTVAEE 88
>At2g30960.1 68415.m03776 expressed protein
Length = 260
Score = 28.7 bits (61), Expect = 3.5
Identities = 31/106 (29%), Positives = 53/106 (50%), Gaps = 7/106 (6%)
Frame = +3
Query: 174 VLPPPSEVVENG--LKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSI----AKR-KTWSK 332
++PP S V EN +K V + K +I ++ IE + + SI AK KT +K
Sbjct: 160 IVPPESVVTENNTPVKDVVPVYWMKATKSKEITKSMAIETQFENFSINRGEAKTLKTQTK 219
Query: 333 FGDSASDKPGPNPATTNVAEDVFMQFITSKEESQRPDDGELDGLKP 470
+ ++P P A+++ M ++ KE+++R DG G+KP
Sbjct: 220 W--RYRNRPMPRGASSST-----MVWV-KKEQNERAGDGIASGVKP 257
>At1g52650.1 68414.m05945 F-box family protein contains F-box domain
Pfam:PF00646
Length = 465
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = -1
Query: 216 FLVHFQPLQKVVEVHLGQSRPHQPRKPEILQ 124
FL HF P + +++H+ ++ P Q R PE+ Q
Sbjct: 404 FLEHF-PCLREIKIHMEENGPTQLRVPEVSQ 433
>At5g57170.1 68418.m07141 macrophage migration inhibitory factor
family protein / MIF family protein contains Pfam
profile: PF01187 Macrophage migration inhibitory
factor(MIF)
Length = 115
Score = 27.5 bits (58), Expect = 8.2
Identities = 10/35 (28%), Positives = 23/35 (65%)
Frame = -3
Query: 376 VAGLGPGLSLAESPNLLQVLRLAILFETTRFSILY 272
+ GLGPG++ S + ++L++ + +++RF I +
Sbjct: 66 IGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKF 100
>At2g36350.1 68415.m04461 protein kinase, putative similar to
protein kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 949
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/54 (27%), Positives = 28/54 (51%)
Frame = +3
Query: 153 EVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAK 314
E+EID+ PP V+E+ L + ++ + V T +++K +VS + K
Sbjct: 266 EIEIDENHTSPPHMVIEDQLVEIDKHVTSLPSCSGSKVDTEELDKSIVSSARVK 319
>At1g76760.1 68414.m08933 thioredoxin family protein similar to
thioredoxin CH2, M-type, chloroplast precursor GB:P23400
SP|P23400 [Chlamydomonas reinhardtii]; contains Pfam
profile: PF00085 Thioredoxin
Length = 172
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +3
Query: 111 DMPVAEEFQASWADEVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIE 284
D PV ++ A+W + +L SE +++ +++V K D + K I YKIE
Sbjct: 81 DKPVLVDYYATWCGPCQFMVPILNEVSETLKDKIQVV---KIDTE-KYPSIANKYKIE 134
>At1g67370.1 68414.m07669 meiotic asynaptic mutant 1 (ASY1)
identical to meiotic asynaptic mutant 1 [Arabidopsis
thaliana] GI:7939627; contains Pfam profiles PF02301:
DNA-binding HORMA domain, PF04433: SWIRM domain
Length = 596
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +3
Query: 339 DSASDKPGPNPATTNVAEDVFMQFITSKEESQRPDDGELD 458
DS D P+ + + +++ QFI + E Q DDGE+D
Sbjct: 252 DSVHDDQ-PSDSDSEISQTQENQFIVAPVEKQDDDDGEVD 290
>At1g57820.2 68414.m06561 zinc finger (C3HC4-type RING finger)
family protein low similarity to nuclear protein np95
[Mus musculus] GI:4220590; contains Pfam profiles
PF02182: YDG/SRA domain, PF00097: Zinc finger, C3HC4
type (RING finger), PF00628: PHD-finger
Length = 642
Score = 27.5 bits (58), Expect = 8.2
Identities = 19/94 (20%), Positives = 43/94 (45%)
Frame = +3
Query: 201 ENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATT 380
E G++ Y+ + +KV + ++K+ + + + + W+ + +P PN
Sbjct: 386 EEGVRYDGVYRIEKCWRKVGVQGSFKVCRYLFVRCDNEPAPWTSDENGDRPRPIPNIPEL 445
Query: 381 NVAEDVFMQFITSKEESQRPDDGELDGLKPPSSN 482
N+A D+F + T + +G +KPP ++
Sbjct: 446 NMATDLFERKETPSWDFDE-GEGCWKWMKPPPAS 478
>At1g57820.1 68414.m06560 zinc finger (C3HC4-type RING finger)
family protein low similarity to nuclear protein np95
[Mus musculus] GI:4220590; contains Pfam profiles
PF02182: YDG/SRA domain, PF00097: Zinc finger, C3HC4
type (RING finger), PF00628: PHD-finger
Length = 645
Score = 27.5 bits (58), Expect = 8.2
Identities = 19/94 (20%), Positives = 43/94 (45%)
Frame = +3
Query: 201 ENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATT 380
E G++ Y+ + +KV + ++K+ + + + + W+ + +P PN
Sbjct: 389 EEGVRYDGVYRIEKCWRKVGVQGSFKVCRYLFVRCDNEPAPWTSDENGDRPRPIPNIPEL 448
Query: 381 NVAEDVFMQFITSKEESQRPDDGELDGLKPPSSN 482
N+A D+F + T + +G +KPP ++
Sbjct: 449 NMATDLFERKETPSWDFDE-GEGCWKWMKPPPAS 481
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,652,992
Number of Sequences: 28952
Number of extensions: 240582
Number of successful extensions: 721
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 706
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 717
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -