Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_M02
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g25550.1 68417.m03683 expressed protein 213 9e-56
At4g29820.1 68417.m04245 expressed protein 159 2e-39
At5g63600.1 68418.m07985 flavonol synthase, putative similar to ... 37 0.010
At1g71860.2 68414.m08304 protein tyrosine phosphatase 1 (PTP1) i... 30 1.2
At1g71860.1 68414.m08305 protein tyrosine phosphatase 1 (PTP1) i... 30 1.2
At5g03770.1 68418.m00341 3-deoxy-D-manno-octulosonic acid transf... 29 2.7
At5g63590.1 68418.m07983 flavonol synthase, putative similar to ... 29 3.6
At4g12720.3 68417.m01998 MutT/nudix family protein similar to SP... 27 8.2
At4g12720.2 68417.m01997 MutT/nudix family protein similar to SP... 27 8.2
At4g12720.1 68417.m01996 MutT/nudix family protein similar to SP... 27 8.2
At4g11330.1 68417.m01829 mitogen-activated protein kinase, putat... 27 8.2
At1g65450.1 68414.m07426 transferase family protein low similari... 27 8.2
>At4g25550.1 68417.m03683 expressed protein
Length = 200
Score = 213 bits (520), Expect = 9e-56
Identities = 96/169 (56%), Positives = 118/169 (69%), Gaps = 1/169 (0%)
Frame = +3
Query: 150 LTLNRSINLYPLTNYTFGTKEPLFEKDASVPARFQRMREEFCKIGMRRSVEGVLLVHEHG 329
+ +++ +N YPL+NY+FGTKEP EKD SV R RM+ + K GMR SVEG+LLV EH
Sbjct: 1 MAMSQVVNTYPLSNYSFGTKEPKLEKDTSVADRLARMKINYMKEGMRTSVEGILLVQEHN 60
Query: 330 LPHVLLLQLGTAFFKLPGGELNPGEDEIDGLKRLLTETL-GRQDGVKQEWLIEXTIGNWW 506
PH+LLLQ+G F KLPGG L PGE+E DGLKR LT L G + +W + + WW
Sbjct: 61 HPHILLLQIGNTFCKLPGGRLKPGENEADGLKRKLTSKLGGNSAALVPDWTVGECVATWW 120
Query: 507 RPNFEPPQYPYIPPHITKPKEHKRLFLVQLQDRALFAVPKNYKLVAAPL 653
RPNFE YPY PPHITKPKE KRL++V L ++ FAVPKN KL+A PL
Sbjct: 121 RPNFETMMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPL 169
>At4g29820.1 68417.m04245 expressed protein
Length = 222
Score = 159 bits (385), Expect = 2e-39
Identities = 79/163 (48%), Positives = 102/163 (62%), Gaps = 1/163 (0%)
Frame = +3
Query: 168 INLYPLTNYTFGTKEPLFEKDASVPARFQRMREEFCKIGMRRSVEGVLLVHEHGLPHVLL 347
++LYPL++Y FG+KE L KD + R R++ + G+R VE VLLV PHVLL
Sbjct: 29 VDLYPLSSYYFGSKEALRVKDEIISDRVIRLKSNYAAHGLRTCVEAVLLVELFKHPHVLL 88
Query: 348 LQLGTAFFKLPGGELNPGEDEIDGLKRLLTETLGRQDGV-KQEWLIEXTIGNWWRPNFEP 524
LQ + FKLPGG L PGE +I+GLKR L L + V + + IG WWRPNFE
Sbjct: 89 LQYRNSIFKLPGGRLRPGESDIEGLKRKLASKLSVNENVGVSGYEVGECIGMWWRPNFET 148
Query: 525 PQYPYIPPHITKPKEHKRLFLVQLQDRALFAVPKNYKLVAAPL 653
YP++PP+I PKE +LFLV+L F VPKN+KL+A PL
Sbjct: 149 LMYPFLPPNIKHPKECTKLFLVRLPVHQQFVVPKNFKLLAVPL 191
>At5g63600.1 68418.m07985 flavonol synthase, putative similar to
SP|Q96330; contains Pfam profile PF03171: 2OG-Fe(II)
oxygenase superfamily
Length = 325
Score = 37.1 bits (82), Expect = 0.010
Identities = 31/93 (33%), Positives = 42/93 (45%), Gaps = 7/93 (7%)
Frame = +3
Query: 303 GVLLVHEHGLPHVLLLQL---GTAFFKLPGGELN--PGEDEIDGLKRLLTETLGRQDGVK 467
GV V HG+P L+ QL GT FF+LP E E++ +G K+ + D
Sbjct: 58 GVFQVVNHGIPTELMRQLQMVGTQFFELPDAEKETVAKEEDFEGYKKNYLGGINNWDEHL 117
Query: 468 QEWLIEXTIGN--WWRPNFEPPQYPYIPPHITK 560
L +I N +W N PPQY + TK
Sbjct: 118 FHRLSPPSIINYKYWPKN--PPQYREVTEEYTK 148
>At1g71860.2 68414.m08304 protein tyrosine phosphatase 1 (PTP1)
identical to protein tyrosine phosphatase 1 GI:3170531
from [Arabidopsis thaliana]; contains Pfam profile:
PF00102 protein-tyrosine phosphatase
Length = 277
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +3
Query: 66 QFKGLPANKQWPARPGLQHQISQNPSMNLTLNRSINLYP 182
+F GL AN+ WP+ L ++ N S+N+ NR ++ P
Sbjct: 61 EFTGLQANRMWPSELLLNSTVAMN-SVNVEKNRYSDVVP 98
>At1g71860.1 68414.m08305 protein tyrosine phosphatase 1 (PTP1)
identical to protein tyrosine phosphatase 1 GI:3170531
from [Arabidopsis thaliana]; contains Pfam profile:
PF00102 protein-tyrosine phosphatase
Length = 340
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +3
Query: 66 QFKGLPANKQWPARPGLQHQISQNPSMNLTLNRSINLYP 182
+F GL AN+ WP+ L ++ N S+N+ NR ++ P
Sbjct: 61 EFTGLQANRMWPSELLLNSTVAMN-SVNVEKNRYSDVVP 98
>At5g03770.1 68418.m00341 3-deoxy-D-manno-octulosonic acid
transferase-related similar to
3-deoxy-D-manno-octulosonic acid transferase,
Escherichia coli, PIR:JU0467; contains Pfam profile
PF04413: 3-Deoxy-D-manno-octulosonic-acid transferase
(kdotransferase)
Length = 447
Score = 29.1 bits (62), Expect = 2.7
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = +3
Query: 24 YAVLLKIRSNIRWQQFKGLPANKQWPARPGLQHQISQNPSMNLTLNRSINL 176
Y V I +IRW++ +GL +WP R G H + P +L +++L
Sbjct: 16 YGVSPLIHLHIRWRRLRGLEHFSRWPERFG--HPSAVRPPGSLIWFHAVSL 64
>At5g63590.1 68418.m07983 flavonol synthase, putative similar to
SP|Q96330 Flavonol synthase 1 (EC 1.14.11.-) (FLS 1)
{Arabidopsis thaliana}; contains Pfam profile PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family
Length = 308
Score = 28.7 bits (61), Expect = 3.6
Identities = 26/107 (24%), Positives = 43/107 (40%), Gaps = 4/107 (3%)
Frame = +3
Query: 303 GVLLVHEHGLPHVL---LLQLGTAFFKLPGGELNPGEDEIDGLKRLLTETLGRQDGVKQE 473
G+ V HG+P L LLQ+G FF+LP E D L T ++D +
Sbjct: 39 GIFQVVNHGIPTELILRLLQVGMEFFELPETEKEAVAKPEDSLDIEGYRTKYQKDLEGRN 98
Query: 474 WLIEXTIGNWWRPN-FEPPQYPYIPPHITKPKEHKRLFLVQLQDRAL 611
++ W P+ +P PP + E + +L ++ +
Sbjct: 99 AWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVNEEYASHIKKLSEKIM 145
>At4g12720.3 68417.m01998 MutT/nudix family protein similar to
SP|P53370 Nucleoside diphosphate-linked moiety X motif 6
{Homo sapiens}; contains Pfam profile PF00293: NUDIX
domain
Length = 282
Score = 27.5 bits (58), Expect = 8.2
Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 6/55 (10%)
Frame = +3
Query: 303 GVLLVHEHGLPHVLLLQLGTAFFK------LPGGELNPGEDEIDGLKRLLTETLG 449
G L+++++ VL++Q + FFK LP G +N GED G+ R + E G
Sbjct: 107 GALVINKN-TKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETG 160
>At4g12720.2 68417.m01997 MutT/nudix family protein similar to
SP|P53370 Nucleoside diphosphate-linked moiety X motif 6
{Homo sapiens}; contains Pfam profile PF00293: NUDIX
domain
Length = 282
Score = 27.5 bits (58), Expect = 8.2
Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 6/55 (10%)
Frame = +3
Query: 303 GVLLVHEHGLPHVLLLQLGTAFFK------LPGGELNPGEDEIDGLKRLLTETLG 449
G L+++++ VL++Q + FFK LP G +N GED G+ R + E G
Sbjct: 107 GALVINKN-TKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETG 160
>At4g12720.1 68417.m01996 MutT/nudix family protein similar to
SP|P53370 Nucleoside diphosphate-linked moiety X motif 6
{Homo sapiens}; contains Pfam profile PF00293: NUDIX
domain
Length = 282
Score = 27.5 bits (58), Expect = 8.2
Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 6/55 (10%)
Frame = +3
Query: 303 GVLLVHEHGLPHVLLLQLGTAFFK------LPGGELNPGEDEIDGLKRLLTETLG 449
G L+++++ VL++Q + FFK LP G +N GED G+ R + E G
Sbjct: 107 GALVINKN-TKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETG 160
>At4g11330.1 68417.m01829 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK5) similar to mitogen-activated
protein kinase homolog 5 (AtMPK5)[Arabidopsis thaliana]
SWISS-PROT:Q39025; PMID:12119167; possible internal
deletion at position 161, missing one A residue;
reference GI:457401
Length = 250
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3
Query: 390 LNPGEDEIDGLKRLLTETLGRQDGVKQEWL 479
L PG+D + LK L+TE +G DG E+L
Sbjct: 117 LFPGKDYVHQLK-LITELIGSPDGASLEFL 145
>At1g65450.1 68414.m07426 transferase family protein low similarity
to anthranilate N-hydroxycinnamoyl/benzoyltransferase
Dianthus caryophyllus GI:3288180, GI:2239091; contains
Pfam profile PF02458 transferase family
Length = 286
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/38 (28%), Positives = 19/38 (50%)
Frame = +3
Query: 483 EXTIGNWWRPNFEPPQYPYIPPHITKPKEHKRLFLVQL 596
E + +WW+ F +YP+ P + P + R +V L
Sbjct: 215 EVLVSSWWKLGFAEVEYPWGKPKYSCPVVYHRKDIVLL 252
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,078,473
Number of Sequences: 28952
Number of extensions: 294563
Number of successful extensions: 879
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 851
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 877
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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