BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_L08 (653 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g42220.1 68418.m05139 ubiquitin family protein contains INTER... 33 0.17 At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam ... 30 1.5 At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu... 27 8.2 At1g66340.1 68414.m07534 ethylene receptor 1 (ETR1) identical to... 27 8.2 >At5g42220.1 68418.m05139 ubiquitin family protein contains INTERPRO:IPR000626 ubiquitin domain Length = 879 Score = 33.1 bits (72), Expect = 0.17 Identities = 17/77 (22%), Positives = 36/77 (46%) Frame = +2 Query: 260 SKMKITVKQLQGGECLIXIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQM 439 S +++ +K L + + + K +A + +PV QQ+ DDH + Sbjct: 22 STLELNIKTLDSRTYTFQVNKNETVLLFKEKIASETGVPVGQQRLIFRGRVLKDDHPLSE 81 Query: 440 YPNIKEXTKLNLVVKKP 490 Y +++ L+L+V++P Sbjct: 82 Y-HLENGHTLHLIVRQP 97 >At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam profile: PF00240 ubiquitin family Length = 158 Score = 29.9 bits (64), Expect = 1.5 Identities = 20/71 (28%), Positives = 33/71 (46%) Frame = +2 Query: 266 MKITVKQLQGGECLIXIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYP 445 M I +K L G ++ + I +K + K IPV+QQ+ D T+ Y Sbjct: 86 MFIFIKTLTGRRIVLEVENCDTIENIKAKIQDKQGIPVDQQRLIFKGKQLEDGLTLADY- 144 Query: 446 NIKEXTKLNLV 478 NI+ + L+L+ Sbjct: 145 NIQNDSILHLI 155 >At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis thaliana] (Genetics 139 (2), 921-939 (1995)) Length = 631 Score = 27.5 bits (58), Expect = 8.2 Identities = 19/73 (26%), Positives = 35/73 (47%) Frame = +2 Query: 266 MKITVKQLQGGECLIXIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYP 445 ++I K L + + S I +K + K IP++QQ+ D T+ Y Sbjct: 3 IQIYAKTLTEKTITLDVETSDSIHNVKAKIQNKEGIPLDQQRLIFAGKQLEDGLTLADY- 61 Query: 446 NIKEXTKLNLVVK 484 NI++ + L+LV++ Sbjct: 62 NIQKESTLHLVLR 74 >At1g66340.1 68414.m07534 ethylene receptor 1 (ETR1) identical to GB:P49333 from [Arabidopsis thaliana] (Science 262 (5133), 539-544 (1993)) Length = 738 Score = 27.5 bits (58), Expect = 8.2 Identities = 14/53 (26%), Positives = 28/53 (52%) Frame = -3 Query: 162 QQRLMIQESVQRMNELKLVLSVNIRS*TEINDGE*SQRIRVYNKHQVVRKSLN 4 +QRLM+ E++ + + L L ++ + + DG + +N H + R+ LN Sbjct: 377 EQRLMV-ETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLHTLFREVLN 428 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,210,576 Number of Sequences: 28952 Number of extensions: 192920 Number of successful extensions: 392 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 386 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 392 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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