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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_L08
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g42220.1 68418.m05139 ubiquitin family protein contains INTER...    33   0.17 
At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam ...    30   1.5  
At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu...    27   8.2  
At1g66340.1 68414.m07534 ethylene receptor 1 (ETR1) identical to...    27   8.2  

>At5g42220.1 68418.m05139 ubiquitin family protein contains
           INTERPRO:IPR000626 ubiquitin domain
          Length = 879

 Score = 33.1 bits (72), Expect = 0.17
 Identities = 17/77 (22%), Positives = 36/77 (46%)
 Frame = +2

Query: 260 SKMKITVKQLQGGECLIXIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQM 439
           S +++ +K L        +  +  +   K  +A +  +PV QQ+         DDH +  
Sbjct: 22  STLELNIKTLDSRTYTFQVNKNETVLLFKEKIASETGVPVGQQRLIFRGRVLKDDHPLSE 81

Query: 440 YPNIKEXTKLNLVVKKP 490
           Y +++    L+L+V++P
Sbjct: 82  Y-HLENGHTLHLIVRQP 97


>At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam
           profile: PF00240 ubiquitin family
          Length = 158

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 20/71 (28%), Positives = 33/71 (46%)
 Frame = +2

Query: 266 MKITVKQLQGGECLIXIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYP 445
           M I +K L G   ++ +     I  +K  +  K  IPV+QQ+         D  T+  Y 
Sbjct: 86  MFIFIKTLTGRRIVLEVENCDTIENIKAKIQDKQGIPVDQQRLIFKGKQLEDGLTLADY- 144

Query: 446 NIKEXTKLNLV 478
           NI+  + L+L+
Sbjct: 145 NIQNDSILHLI 155


>At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to
           polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis
           thaliana] (Genetics 139 (2), 921-939 (1995))
          Length = 631

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 19/73 (26%), Positives = 35/73 (47%)
 Frame = +2

Query: 266 MKITVKQLQGGECLIXIYPSMLISELKRHVARKLHIPVEQQKXXXXXXXXXDDHTIQMYP 445
           ++I  K L      + +  S  I  +K  +  K  IP++QQ+         D  T+  Y 
Sbjct: 3   IQIYAKTLTEKTITLDVETSDSIHNVKAKIQNKEGIPLDQQRLIFAGKQLEDGLTLADY- 61

Query: 446 NIKEXTKLNLVVK 484
           NI++ + L+LV++
Sbjct: 62  NIQKESTLHLVLR 74


>At1g66340.1 68414.m07534 ethylene receptor 1 (ETR1) identical to
           GB:P49333 from [Arabidopsis thaliana] (Science 262
           (5133), 539-544 (1993))
          Length = 738

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 14/53 (26%), Positives = 28/53 (52%)
 Frame = -3

Query: 162 QQRLMIQESVQRMNELKLVLSVNIRS*TEINDGE*SQRIRVYNKHQVVRKSLN 4
           +QRLM+ E++ + + L   L  ++   + + DG     +  +N H + R+ LN
Sbjct: 377 EQRLMV-ETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLHTLFREVLN 428


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,210,576
Number of Sequences: 28952
Number of extensions: 192920
Number of successful extensions: 392
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 386
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 392
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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