SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_K22
         (629 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAPYUG7.04c |rpb9||DNA-directed RNA polymerase II complex subun...   114   1e-26
SPAC22A12.05 |rpc11||DNA-directed RNA polymerase III complex sub...    47   3e-06
SPCC1259.03 |rpa12||DNA-directed RNA polymerase complex I subuni...    36   0.005
SPAC20H4.03c |tfs1||transcription elongation factor TFIIS |Schiz...    36   0.006
SPBC16G5.18 |erg24||C-14 sterol reductase Erg24|Schizosaccharomy...    26   3.9  
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||...    26   3.9  
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual      26   3.9  
SPAC23H3.02c |ini1||RING finger-like protein Ini1|Schizosaccharo...    26   5.2  
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa...    25   9.0  

>SPAPYUG7.04c |rpb9||DNA-directed RNA polymerase II complex subunit
           Rpb9 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 113

 Score =  114 bits (274), Expect = 1e-26
 Identities = 51/109 (46%), Positives = 71/109 (65%)
 Frame = +1

Query: 238 QFCQECNNMLYPREDKNNKVLLYACRNCDYKQLADSNCVYVNKIMHXVXELTHINPDVXS 417
           Q+C ECNNMLYPREDK ++VL  ACRNCDY ++A ++ VY +++     E T ++ D  +
Sbjct: 5   QYCIECNNMLYPREDKVDRVLRLACRNCDYSEIAATSKVYRHELQSSNVENTTVSHDAST 64

Query: 418 DPTLPXTKDHMCPKCNHREAVFFQGQTXRAEQXMXLYYVCTXCKHXWTE 564
           DPTLP + D  CP+C+  EAVF+Q  + R +  M L YVC  C   + E
Sbjct: 65  DPTLPRS-DKECPRCHQHEAVFYQTHSRRGDTMMTLIYVCVHCGFAFEE 112


>SPAC22A12.05 |rpc11||DNA-directed RNA polymerase III complex
           subunit Rpc11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 109

 Score = 46.8 bits (106), Expect = 3e-06
 Identities = 32/112 (28%), Positives = 47/112 (41%), Gaps = 2/112 (1%)
 Frame = +1

Query: 235 IQFCQECNNMLYPREDKNNKVLLYACRNCDYKQLADSNCVYVNK--IMHXVXELTHINPD 408
           +QFC  C N L    D+  +   + CR C Y     S  +Y         V ++      
Sbjct: 1   MQFCPTCGNHLIVAVDEEGRNA-FDCRTCPY-HFPISTFLYSRHEFAQKEVDDVLGGEEA 58

Query: 409 VXSDPTLPXTKDHMCPKCNHREAVFFQGQTXRAEQXMXLYYVCTXCKHXWTE 564
             S+     T ++   KC++  A FFQ Q   A++ M  +Y CT CK  W E
Sbjct: 59  FESNQQTEVTCENT--KCDNNRAYFFQLQIRSADEPMSTFYRCTKCKFQWRE 108


>SPCC1259.03 |rpa12||DNA-directed RNA polymerase complex I subunit
           Rpa12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 119

 Score = 35.9 bits (79), Expect = 0.005
 Identities = 27/113 (23%), Positives = 41/113 (36%), Gaps = 6/113 (5%)
 Frame = +1

Query: 241 FCQECNNMLYPREDKNNKVLLYACRNCD--YKQLADSNCVYVNK----IMHXVXELTHIN 402
           FC EC N+L     ++       C  C   Y     +N V   K          +L H  
Sbjct: 9   FCSECGNLL-----ESTTAQWTTCDQCQSVYPSEQFANLVVETKSSASAFPSALKLKHSI 63

Query: 403 PDVXSDPTLPXTKDHMCPKCNHREAVFFQGQTXRAEQXMXLYYVCTXCKHXWT 561
             V S      T +  CPKC +    F   Q   A++   ++Y C  C + ++
Sbjct: 64  VQVESQKEEAATIEEKCPKCGNDHMTFHTLQLRSADEGSTVFYECPRCAYKFS 116


>SPAC20H4.03c |tfs1||transcription elongation factor TFIIS
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 293

 Score = 35.5 bits (78), Expect = 0.006
 Identities = 12/36 (33%), Positives = 20/36 (55%)
 Frame = +1

Query: 451 CPKCNHREAVFFQGQTXRAEQXMXLYYVCTXCKHXW 558
           C KC  ++  ++Q QT  A++ M  +  CT C + W
Sbjct: 255 CGKCKQKKVSYYQMQTRSADEPMTTFCECTVCGNRW 290


>SPBC16G5.18 |erg24||C-14 sterol reductase Erg24|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 424

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 13/35 (37%), Positives = 17/35 (48%)
 Frame = -3

Query: 561 CPXMFTXCTHIIQXHXLFGAXRLALKEYCFSVITL 457
           CP  F+  +HI +   LF    L L    FS +TL
Sbjct: 44  CPAKFSKISHIFKKTPLFDQKSLILYLLWFSTLTL 78


>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1158

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%)
 Frame = +1

Query: 160 HFK-INSKEMSAISLSRKDGGPG 225
           HF+  +S   S  SLSRKD GPG
Sbjct: 67  HFRPASSLSFSPSSLSRKDSGPG 89


>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 11/34 (32%), Positives = 20/34 (58%)
 Frame = -2

Query: 352 RNLNPQVVYNHNFCTRTAKLCYFYLRAGKAYCYI 251
           + L+PQ  +  + C++  +   FY R GK YC++
Sbjct: 276 KKLHPQC-FKCDTCSQNLEHVGFYYREGKFYCHL 308


>SPAC23H3.02c |ini1||RING finger-like protein
           Ini1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 9/22 (40%), Positives = 11/22 (50%)
 Frame = +1

Query: 214 GGPGYVGIQFCQECNNMLYPRE 279
           G PG     +C EC  M Y R+
Sbjct: 62  GAPGVSDCYYCSECTRMEYDRD 83


>SPAC19A8.08 |upf2||nonsense-mediated decay protein
           Upf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1049

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 16/53 (30%), Positives = 22/53 (41%), Gaps = 2/53 (3%)
 Frame = +2

Query: 218 VLVMSAYNFVKNVTICFTRA--KIKITKFCCTRAEIVIINNLRIQIAFTSTKL 370
           +  +S   F+  +T        K K TK   +  +IV   NLR   AFT   L
Sbjct: 67  IKTLSLKKFIPEITAAIVEGMMKCKATKDILSSVKIVWALNLRFSTAFTGPML 119


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,177,267
Number of Sequences: 5004
Number of extensions: 38114
Number of successful extensions: 98
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 94
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 96
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -