BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_K22 (629 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPYUG7.04c |rpb9||DNA-directed RNA polymerase II complex subun... 114 1e-26 SPAC22A12.05 |rpc11||DNA-directed RNA polymerase III complex sub... 47 3e-06 SPCC1259.03 |rpa12||DNA-directed RNA polymerase complex I subuni... 36 0.005 SPAC20H4.03c |tfs1||transcription elongation factor TFIIS |Schiz... 36 0.006 SPBC16G5.18 |erg24||C-14 sterol reductase Erg24|Schizosaccharomy... 26 3.9 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 3.9 SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 26 3.9 SPAC23H3.02c |ini1||RING finger-like protein Ini1|Schizosaccharo... 26 5.2 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 9.0 >SPAPYUG7.04c |rpb9||DNA-directed RNA polymerase II complex subunit Rpb9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 113 Score = 114 bits (274), Expect = 1e-26 Identities = 51/109 (46%), Positives = 71/109 (65%) Frame = +1 Query: 238 QFCQECNNMLYPREDKNNKVLLYACRNCDYKQLADSNCVYVNKIMHXVXELTHINPDVXS 417 Q+C ECNNMLYPREDK ++VL ACRNCDY ++A ++ VY +++ E T ++ D + Sbjct: 5 QYCIECNNMLYPREDKVDRVLRLACRNCDYSEIAATSKVYRHELQSSNVENTTVSHDAST 64 Query: 418 DPTLPXTKDHMCPKCNHREAVFFQGQTXRAEQXMXLYYVCTXCKHXWTE 564 DPTLP + D CP+C+ EAVF+Q + R + M L YVC C + E Sbjct: 65 DPTLPRS-DKECPRCHQHEAVFYQTHSRRGDTMMTLIYVCVHCGFAFEE 112 >SPAC22A12.05 |rpc11||DNA-directed RNA polymerase III complex subunit Rpc11|Schizosaccharomyces pombe|chr 1|||Manual Length = 109 Score = 46.8 bits (106), Expect = 3e-06 Identities = 32/112 (28%), Positives = 47/112 (41%), Gaps = 2/112 (1%) Frame = +1 Query: 235 IQFCQECNNMLYPREDKNNKVLLYACRNCDYKQLADSNCVYVNK--IMHXVXELTHINPD 408 +QFC C N L D+ + + CR C Y S +Y V ++ Sbjct: 1 MQFCPTCGNHLIVAVDEEGRNA-FDCRTCPY-HFPISTFLYSRHEFAQKEVDDVLGGEEA 58 Query: 409 VXSDPTLPXTKDHMCPKCNHREAVFFQGQTXRAEQXMXLYYVCTXCKHXWTE 564 S+ T ++ KC++ A FFQ Q A++ M +Y CT CK W E Sbjct: 59 FESNQQTEVTCENT--KCDNNRAYFFQLQIRSADEPMSTFYRCTKCKFQWRE 108 >SPCC1259.03 |rpa12||DNA-directed RNA polymerase complex I subunit Rpa12|Schizosaccharomyces pombe|chr 3|||Manual Length = 119 Score = 35.9 bits (79), Expect = 0.005 Identities = 27/113 (23%), Positives = 41/113 (36%), Gaps = 6/113 (5%) Frame = +1 Query: 241 FCQECNNMLYPREDKNNKVLLYACRNCD--YKQLADSNCVYVNK----IMHXVXELTHIN 402 FC EC N+L ++ C C Y +N V K +L H Sbjct: 9 FCSECGNLL-----ESTTAQWTTCDQCQSVYPSEQFANLVVETKSSASAFPSALKLKHSI 63 Query: 403 PDVXSDPTLPXTKDHMCPKCNHREAVFFQGQTXRAEQXMXLYYVCTXCKHXWT 561 V S T + CPKC + F Q A++ ++Y C C + ++ Sbjct: 64 VQVESQKEEAATIEEKCPKCGNDHMTFHTLQLRSADEGSTVFYECPRCAYKFS 116 >SPAC20H4.03c |tfs1||transcription elongation factor TFIIS |Schizosaccharomyces pombe|chr 1|||Manual Length = 293 Score = 35.5 bits (78), Expect = 0.006 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +1 Query: 451 CPKCNHREAVFFQGQTXRAEQXMXLYYVCTXCKHXW 558 C KC ++ ++Q QT A++ M + CT C + W Sbjct: 255 CGKCKQKKVSYYQMQTRSADEPMTTFCECTVCGNRW 290 >SPBC16G5.18 |erg24||C-14 sterol reductase Erg24|Schizosaccharomyces pombe|chr 2|||Manual Length = 424 Score = 26.2 bits (55), Expect = 3.9 Identities = 13/35 (37%), Positives = 17/35 (48%) Frame = -3 Query: 561 CPXMFTXCTHIIQXHXLFGAXRLALKEYCFSVITL 457 CP F+ +HI + LF L L FS +TL Sbjct: 44 CPAKFSKISHIFKKTPLFDQKSLILYLLWFSTLTL 78 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 3.9 Identities = 13/23 (56%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 160 HFK-INSKEMSAISLSRKDGGPG 225 HF+ +S S SLSRKD GPG Sbjct: 67 HFRPASSLSFSPSSLSRKDSGPG 89 >SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 26.2 bits (55), Expect = 3.9 Identities = 11/34 (32%), Positives = 20/34 (58%) Frame = -2 Query: 352 RNLNPQVVYNHNFCTRTAKLCYFYLRAGKAYCYI 251 + L+PQ + + C++ + FY R GK YC++ Sbjct: 276 KKLHPQC-FKCDTCSQNLEHVGFYYREGKFYCHL 308 >SPAC23H3.02c |ini1||RING finger-like protein Ini1|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 25.8 bits (54), Expect = 5.2 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +1 Query: 214 GGPGYVGIQFCQECNNMLYPRE 279 G PG +C EC M Y R+ Sbjct: 62 GAPGVSDCYYCSECTRMEYDRD 83 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.0 bits (52), Expect = 9.0 Identities = 16/53 (30%), Positives = 22/53 (41%), Gaps = 2/53 (3%) Frame = +2 Query: 218 VLVMSAYNFVKNVTICFTRA--KIKITKFCCTRAEIVIINNLRIQIAFTSTKL 370 + +S F+ +T K K TK + +IV NLR AFT L Sbjct: 67 IKTLSLKKFIPEITAAIVEGMMKCKATKDILSSVKIVWALNLRFSTAFTGPML 119 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,177,267 Number of Sequences: 5004 Number of extensions: 38114 Number of successful extensions: 98 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 94 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 96 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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