Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_J08
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g15750.1 68418.m01842 RNA-binding S4 domain-containing protei... 186 1e-47
At5g39850.1 68418.m04829 40S ribosomal protein S9 (RPS9C) 40S ri... 58 5e-09
At5g15200.1 68418.m01781 40S ribosomal protein S9 (RPS9B) 40S ri... 55 4e-08
At1g45545.1 68414.m05199 hypothetical protein contains Pfam prof... 31 0.88
At5g41620.1 68418.m05057 expressed protein weak similarity to mi... 30 1.5
At3g47460.1 68416.m05161 SMC2-like condensin, putative similar t... 29 2.7
At2g27920.2 68415.m03383 serine carboxypeptidase S10 family prot... 29 2.7
At2g27920.1 68415.m03384 serine carboxypeptidase S10 family prot... 29 2.7
At5g22260.1 68418.m02593 male sterility 1 protein, putative (MS1... 29 3.6
At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 29 3.6
At1g79280.1 68414.m09242 expressed protein weak similarity to Nu... 29 3.6
At4g04110.1 68417.m00581 Toll-Interleukin-Resistance (TIR) domai... 28 4.7
At3g02990.1 68416.m00294 heat shock factor protein 2 (HSF2) / he... 27 8.2
At1g64180.1 68414.m07270 intracellular protein transport protein... 27 8.2
>At5g15750.1 68418.m01842 RNA-binding S4 domain-containing protein
40S RIBOSOMAL PROTEINs - different species
Length = 182
Score = 186 bits (453), Expect = 1e-47
Identities = 86/173 (49%), Positives = 119/173 (68%)
Frame = +1
Query: 136 VRKLKFHEGKLLKKVXFISWKLDNNISEVKVMKKFCIQKREDYTKYNKLSRDIRELANKI 315
+RKLK+HE KL+KKV F+ WK + N E ++ ++ + R+DY KY+ L R +++L N +
Sbjct: 1 MRKLKYHEKKLIKKVNFLEWKREGNHRENEITYRYHMGSRDDYKKYSGLCRMVQKLTNIM 60
Query: 316 KDLDPNNEFRTESSAQLLEKLYQIGLIPTRWDLALATNVSASSFCRRRLPVLMVRNKMSX 495
K +DP + FR + + LLEKLY +G+IPTR L L +S SSFCRRRL ++V K +
Sbjct: 61 KQMDPADPFRIQMTDMLLEKLYNMGVIPTRKSLTLTERLSVSSFCRRRLSTVLVHLKFAE 120
Query: 496 TIKEATKFIEQRHVXVGPEVVKDPAFLVNRSLEDFVTWVDGSAITKHVLXYNE 654
KEA +IEQ HV VGPE + DPAFLV R++EDF+TWVD S I + VL YN+
Sbjct: 121 HHKEAVTYIEQGHVRVGPETITDPAFLVTRNMEDFITWVDSSKIKRKVLEYND 173
>At5g39850.1 68418.m04829 40S ribosomal protein S9 (RPS9C) 40S
ribosomal protein S9 - Chlamydomonas sp.,EMBL:AU066528
Length = 197
Score = 58.0 bits (134), Expect = 5e-09
Identities = 38/124 (30%), Positives = 68/124 (54%), Gaps = 2/124 (1%)
Frame = +1
Query: 214 SEVKVMKKFCIQ-KREDYTKYNKLSRDIRELANKIKDLDPNNEFRTESSAQLLEKLYQIG 390
+E+K++ ++ ++ KRE + LSR IR A ++ LD N R LL ++ + G
Sbjct: 28 AELKLVGEYGLRCKRELWRVQYTLSR-IRNAARELLTLDEKNPRRIFEGEALLRRMNRYG 86
Query: 391 LIP-TRWDLALATNVSASSFCRRRLPVLMVRNKMSXTIKEATKFIEQRHVXVGPEVVKDP 567
L+ T+ L ++ +F RRL ++ ++ M+ +I A I QRH+ VG ++V P
Sbjct: 87 LLDETQNKLDYVLALTVENFLERRLQTIVFKSGMAKSIHHARVLIRQRHIRVGRQLVNIP 146
Query: 568 AFLV 579
+F+V
Sbjct: 147 SFMV 150
>At5g15200.1 68418.m01781 40S ribosomal protein S9 (RPS9B) 40S
ribosomal protein S9, Chlamydomonas sp., EMBL:AU066528
Length = 198
Score = 55.2 bits (127), Expect = 4e-08
Identities = 39/126 (30%), Positives = 67/126 (53%), Gaps = 4/126 (3%)
Frame = +1
Query: 214 SEVKVMKKFCIQ-KREDYTKYNKLSRDIRELANKIKDLDPNNEFRTESSAQLLEKLYQIG 390
SE+K++ ++ ++ KRE + LSR IR A + LD + R LL ++ + G
Sbjct: 28 SELKLVGEYGLRNKRELWRVQYSLSR-IRNAARDLLTLDEKSPRRIFEGEALLRRMNRYG 86
Query: 391 LIP---TRWDLALATNVSASSFCRRRLPVLMVRNKMSXTIKEATKFIEQRHVXVGPEVVK 561
L+ + D LA V +F RRL ++ ++ M+ +I + I QRH+ VG ++V
Sbjct: 87 LLDESQNKLDYVLALTVE--NFLERRLQTIVFKSGMAKSIHHSRVLIRQRHIRVGKQLVN 144
Query: 562 DPAFLV 579
P+F+V
Sbjct: 145 IPSFMV 150
>At1g45545.1 68414.m05199 hypothetical protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827)
Length = 752
Score = 30.7 bits (66), Expect = 0.88
Identities = 16/62 (25%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +1
Query: 205 NNISEVKVMKKFCIQKREDYTKYNKLSRDIRELAN-KIKDLDPNNEFRTESSAQLLEKLY 381
N I ++ K I E+Y + +K + ++ E AN K+ ++ E E +++LE L
Sbjct: 575 NKIEDISSSPKSIIISVEEYYELSKQAHEVEEAANRKLAEIVSKIEVAKEEESRILENLE 634
Query: 382 QI 387
++
Sbjct: 635 EV 636
>At5g41620.1 68418.m05057 expressed protein weak similarity to
microtubule binding protein D-CLIP-190 (GI:2773363)
[Drosophila melanogaster]; weak similarity to
Synaptonemal complex protein 1 (SCP-1 protein)
(Swiss-Prot:Q15431) [Homo sapiens]; weak similarity to
DNA double-strand break repair rad50 ATPase.
(Swiss-Prot:P58301) [Pyrococcus furiosus]
Length = 543
Score = 29.9 bits (64), Expect = 1.5
Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 1/65 (1%)
Frame = +1
Query: 136 VRKLKFHEGKLLKKVXFISWKLDNNISEVKVMKKFCIQKREDYTKYNKLSRDI-RELANK 312
VRK E KL K+ + K+ +SEVK C+++ E +K NK+ + E A
Sbjct: 197 VRKALEDERKLRKRSESLHRKMARELSEVKSSLSNCVKELERGSKSNKMMELLCDEFAKG 256
Query: 313 IKDLD 327
IK +
Sbjct: 257 IKSYE 261
>At3g47460.1 68416.m05161 SMC2-like condensin, putative similar to
SMC2-like condensin (TITAN3) [Arabidopsis thaliana]
GI:14279543; contains Pfam profiles PF02483: SMC family
C-terminal domain, PF02463: RecF/RecN/SMC N terminal
domain
Length = 1171
Score = 29.1 bits (62), Expect = 2.7
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +1
Query: 256 EDYTKYNKLSRDIRELANKIKDLDPNNEFRTESSAQLLEKLYQIGLIPTR 405
E TK+ + + E+ IK+L P T+ AQL K+Y + L R
Sbjct: 678 EAETKFRAHQKSLSEIEANIKELQPLQTKFTDMKAQLELKMYDMSLFLKR 727
>At2g27920.2 68415.m03383 serine carboxypeptidase S10 family protein
similar to retinoid-inducible serine carboxypeptidase
precursor (GI:15146429) [Mus musculus]
Length = 389
Score = 29.1 bits (62), Expect = 2.7
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = +1
Query: 178 VXFISWKLDNNISEVKVMKKFCIQKREDYTKYNKLSRDIRELANKIKDLD 327
V F ++ LD + V + I+K E KY++ D+R L++ ++D++
Sbjct: 194 VDFYNFLLDTGMDPVSLTTSLKIKKEEKIKKYSRYLNDMRSLSD-VEDVE 242
>At2g27920.1 68415.m03384 serine carboxypeptidase S10 family protein
similar to retinoid-inducible serine carboxypeptidase
precursor (GI:15146429) [Mus musculus]
Length = 461
Score = 29.1 bits (62), Expect = 2.7
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = +1
Query: 178 VXFISWKLDNNISEVKVMKKFCIQKREDYTKYNKLSRDIRELANKIKDLD 327
V F ++ LD + V + I+K E KY++ D+R L++ ++D++
Sbjct: 266 VDFYNFLLDTGMDPVSLTTSLKIKKEEKIKKYSRYLNDMRSLSD-VEDVE 314
>At5g22260.1 68418.m02593 male sterility 1 protein, putative (MS1)
identical to male sterility 1 protein [Arabidopsis
thaliana] gi|15554513|emb|CAC69663 PMID:11696184;
contains Pfam profile PF00628: PHD-finger; identical to
cDNA male sterility 1 protein (ms1 gene) GI:15554514
Length = 672
Score = 28.7 bits (61), Expect = 3.6
Identities = 17/65 (26%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
Frame = +1
Query: 139 RKLKFHEGKLLKKVXFISWKLDNNISEVKVMKKFCIQKREDYT---KYNKLSRDIRELAN 309
+ L G+ L I+WK ++ +E+KV + CI ++D T Y + R R++
Sbjct: 496 KTLHLDRGEELGIFCTIAWKCHHHNNEIKVPPQECIVVKKDATLSEVYGEAERVFRDIYW 555
Query: 310 KIKDL 324
+++D+
Sbjct: 556 ELRDV 560
>At3g01360.1 68416.m00057 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 319
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +1
Query: 253 REDYTKYNKLSRDIRELANKIKDLDPNNEFRTESS 357
R+D++KY L ++ L+ D D +E R ES+
Sbjct: 271 RQDHSKYRPLGAELENLSTFTLDSDEEDEVREESN 305
>At1g79280.1 68414.m09242 expressed protein weak similarity to
Nucleoprotein TPR (Swiss-Prot:P12270) [Homo sapiens]
Length = 2111
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +1
Query: 259 DYTKYNKLSRDIRELANKIKDLDPNNEFRTESSAQLLEKLYQIGLI 396
D YN+L ++R+L K+K D + E + LLEK +I L+
Sbjct: 1373 DIADYNRLKDEVRQLEEKLKAKDAHAE---DCKKVLLEKQNKISLL 1415
>At4g04110.1 68417.m00581 Toll-Interleukin-Resistance (TIR)
domain-containing protein domain signature TIR exists,
suggestive of a disease resistance protein.
Length = 239
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = -3
Query: 385 FGKVFPKV-EHLTLS*IHYLDRGLLSCLRAL*YHETIC 275
FGK+F K ++ T I+ R L+ L YH TIC
Sbjct: 151 FGKIFKKTCQNRTEDEINLRRRALIDVANTLGYHSTIC 188
>At3g02990.1 68416.m00294 heat shock factor protein 2 (HSF2) / heat
shock transcription factor 2 (HSTF2) identical to heat
shock transcription factor 2 (HSF2) SP:Q96320 from
[Arabidopsis thaliana]; contains Pfam profile: PF00447
HSF-type DNA-binding domain
Length = 468
Score = 27.5 bits (58), Expect = 8.2
Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 2/57 (3%)
Frame = +1
Query: 187 ISWKLDNNISEVKVMKKFCIQKREDYTKYNKLSRDIREL-ANKIKDLDPNN-EFRTE 351
+SW NN V + +F Q Y K+N S +R+L + +DP+ EF E
Sbjct: 41 VSWSSGNNSFVVWNVPEFAKQFLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANE 97
>At1g64180.1 68414.m07270 intracellular protein transport protein
USO1-related similar to Rap8 (GI:2326183) [Rhynchosciara
americana]; contains weak similarity to
Swiss-Prot:P25386 intracellular protein transport
protein USO1 [Saccharomyces cerevisiae]
Length = 593
Score = 27.5 bits (58), Expect = 8.2
Identities = 20/58 (34%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Frame = +1
Query: 157 EGKLLKKVXFISWKLDNNISEVKVMKKFCIQKREDYTKYNK-LSRDIRELANKIKDLD 327
E KL K+ + KL +SEVK C+++ E T+ K L R E A IK +
Sbjct: 272 ERKLRKRSESLYRKLAQELSEVKSTLSNCVKEMERGTESKKILERLCDEFAKGIKSYE 329
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,411,367
Number of Sequences: 28952
Number of extensions: 260544
Number of successful extensions: 639
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 623
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 639
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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