Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_J06
(645 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 73 2e-13
At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 72 4e-13
At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 68 5e-12
At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 66 1e-11
At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 65 3e-11
At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 48 4e-06
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 46 2e-05
At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 38 0.006
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 38 0.008
At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 35 0.040
At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 35 0.053
At5g67220.1 68418.m08473 nitrogen regulation family protein simi... 29 3.5
At4g17250.1 68417.m02594 expressed protein 28 6.1
At3g59420.1 68416.m06627 receptor protein kinase, putative (ACR4... 28 6.1
At4g35440.1 68417.m05035 voltage-gated chloride channel family p... 27 8.1
At3g24900.1 68416.m03122 disease resistance family protein / LRR... 27 8.1
At2g23190.1 68415.m02770 cytochrome P450, putative Similar to C... 27 8.1
>At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5
[Arabidopsis thaliana] GI:4240122; strong similarity to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 140
Score = 72.5 bits (170), Expect = 2e-13
Identities = 31/58 (53%), Positives = 42/58 (72%)
Frame = +3
Query: 87 EVADSKTATETLFIIDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEFEDVGHSMDAR 260
EV+ +A + +ID VYDV KFLD+HPGG EV++ GKDA+++FEDVGHS A+
Sbjct: 12 EVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSSTAK 69
>At1g26340.1 68414.m03212 cytochrome b5, putative similar to
cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis
thaliana]
Length = 135
Score = 71.7 bits (168), Expect = 4e-13
Identities = 32/62 (51%), Positives = 41/62 (66%)
Frame = +3
Query: 87 EVADSKTATETLFIIDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEFEDVGHSMDARXT 266
E A + +ID VYDV ++DEHPGG +VL+ VAGKDA+++FED GHS DAR
Sbjct: 12 EAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKDAREL 71
Query: 267 HE 272
E
Sbjct: 72 ME 73
>At2g32720.1 68415.m04004 cytochrome b5, putative similar to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 134
Score = 68.1 bits (159), Expect = 5e-12
Identities = 30/62 (48%), Positives = 44/62 (70%)
Frame = +3
Query: 87 EVADSKTATETLFIIDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEFEDVGHSMDARXT 266
EV++ A + +I+ VY+V KFL++HPGG +VL++ GKDA+++FEDVGHS AR
Sbjct: 12 EVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSESAREM 71
Query: 267 HE 272
E
Sbjct: 72 ME 73
>At2g46650.1 68415.m05820 cytochrome b5, putative similar to
cytochome b5 GI:2695711 from [Olea europaea]
Length = 132
Score = 66.5 bits (155), Expect = 1e-11
Identities = 30/58 (51%), Positives = 38/58 (65%)
Frame = +3
Query: 87 EVADSKTATETLFIIDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEFEDVGHSMDAR 260
+VA K + +I VYD+ F+DEHPGG VL+ V GKDAS +FEDV HS DA+
Sbjct: 9 DVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66
>At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to
SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana]
Length = 134
Score = 65.3 bits (152), Expect = 3e-11
Identities = 30/58 (51%), Positives = 38/58 (65%)
Frame = +3
Query: 87 EVADSKTATETLFIIDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEFEDVGHSMDAR 260
EV+ + II VYDV F+D+HPGG EVL++ GKDA+ +FEDVGHS AR
Sbjct: 12 EVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSDTAR 69
>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
(NR1){Arabidopsis thaliana}
Length = 917
Score = 48.4 bits (110), Expect = 4e-06
Identities = 26/67 (38%), Positives = 36/67 (53%)
Frame = +3
Query: 87 EVADSKTATETLFIIDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEFEDVGHSMDARXT 266
EV TA I+ +YD +FL +HPGG + ++ AG D +EEFE + HS A+
Sbjct: 552 EVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAI-HSDKAKKL 610
Query: 267 HEGLRGG 287
E R G
Sbjct: 611 LEDYRIG 617
>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
(NR2) {Arabidopsis thaliana}
Length = 917
Score = 46.0 bits (104), Expect = 2e-05
Identities = 25/67 (37%), Positives = 36/67 (53%)
Frame = +3
Query: 87 EVADSKTATETLFIIDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEFEDVGHSMDARXT 266
EV +A I+ +YD +FL +HPGG + ++ AG D +EEFE + HS A+
Sbjct: 549 EVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAI-HSDKAKKM 607
Query: 267 HEGLRGG 287
E R G
Sbjct: 608 LEDYRIG 614
>At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein
contains InterPro accession IPR001199: Cytochrome b5
Length = 121
Score = 37.9 bits (84), Expect = 0.006
Identities = 18/54 (33%), Positives = 32/54 (59%)
Frame = +3
Query: 87 EVADSKTATETLFIIDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEFEDVGHS 248
EVA + II + VYD+ +++EHPGG + +++ AG D+++ F H+
Sbjct: 53 EVAVHNKRNDCWIIIKDKVYDITSYVEEHPGG-DAILDHAGDDSTDGFFGPQHA 105
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 37.5 bits (83), Expect = 0.008
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = +3
Query: 129 IDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEF 230
I VYDV ++ HPGG ++N+AG+D ++ F
Sbjct: 28 IQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAF 61
>At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1)
identical to delta-8 sphingolipid desaturase GI:3819710
from [Arabidopsis thaliana]; contains Pfam profile
PF00487: Fatty acid desaturase; contains Pfam profile
PF00173: Heme/Steroid binding domain
Length = 449
Score = 35.1 bits (77), Expect = 0.040
Identities = 13/34 (38%), Positives = 22/34 (64%)
Frame = +3
Query: 129 IDNIVYDVHKFLDEHPGGHEVLINVAGKDASEEF 230
I VY+V ++ HPGG V++N+ G+D ++ F
Sbjct: 28 IQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61
>At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein
flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803
Length = 211
Score = 34.7 bits (76), Expect = 0.053
Identities = 13/48 (27%), Positives = 26/48 (54%)
Frame = +3
Query: 78 PAXEVADSKTATETLFIIDNIVYDVHKFLDEHPGGHEVLINVAGKDAS 221
P EV +T ++ VY++ +++ HPGG ++L+ G+D +
Sbjct: 130 PMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGT 177
>At5g67220.1 68418.m08473 nitrogen regulation family protein similar
to unknown protein (gb|AAF51525.1) ; contains Pfam
domain PF01207: Dihydrouridine synthase (Dus); similar
to (SP:P45672) NIFR3-like protein (SP:P45672)
[Azospirillum brasilense]
Length = 423
Score = 28.7 bits (61), Expect = 3.5
Identities = 16/30 (53%), Positives = 19/30 (63%)
Frame = -1
Query: 537 P*LHSRIIR*TTKYRNELATTVPCCDNRQL 448
P LHSRI T KYRN+ TT C ++R L
Sbjct: 118 PMLHSRIFTETEKYRNQEFTT--CKEDRPL 145
>At4g17250.1 68417.m02594 expressed protein
Length = 416
Score = 27.9 bits (59), Expect = 6.1
Identities = 15/32 (46%), Positives = 22/32 (68%), Gaps = 4/32 (12%)
Frame = +2
Query: 446 DSCLLSQHGTVVASSFLYFV-VY---RMILEC 529
D C+ +Q+G V+AS FL F+ VY R++L C
Sbjct: 356 DICIATQYGIVLASKFLRFITVYCSSRILLCC 387
>At3g59420.1 68416.m06627 receptor protein kinase, putative (ACR4)
identical to putative receptor protein kinase ACR4
[Arabidopsis thaliana] GI:20302590; contains protein
kinase domain, Pfam:PF00069
Length = 895
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = -3
Query: 241 PTSSNSSEASLPATLMSTSCPPGCSSRNLCT 149
P S LP STSCPPG +++CT
Sbjct: 361 PCKFTGSHICLPC---STSCPPGMYQKSVCT 388
>At4g35440.1 68417.m05035 voltage-gated chloride channel family
protein contains Pfam profile PF00654: Voltage gated
chloride channel
Length = 710
Score = 27.5 bits (58), Expect = 8.1
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -3
Query: 253 SMLWPTSSNSSEASLPAT 200
S+LWP+SS S SLP T
Sbjct: 244 SVLWPSSSTDSSTSLPNT 261
>At3g24900.1 68416.m03122 disease resistance family protein / LRR
family protein contains leucine rich-repeat domains
Pfam:PF00560, INTERPRO:IPR001611; similar to Cf-2.2
[Lycopersicon pimpinellifolium] gi|1184077|gb|AAC15780
Length = 884
Score = 27.5 bits (58), Expect = 8.1
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +2
Query: 545 HSECVCGVWCDAGSGAQLR 601
HS+ + GVWCD +GA ++
Sbjct: 60 HSDSLNGVWCDNSTGAVMK 78
>At2g23190.1 68415.m02770 cytochrome P450, putative Similar to
Cytochrome P450 91A1 (SP:Q9FG65) [Arabidopsis thaliana];
Length = 543
Score = 27.5 bits (58), Expect = 8.1
Identities = 11/34 (32%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +2
Query: 422 PCST-HIYSDSCLLSQHGTVVASSFLYFVVYRMI 520
PCS HIY L+ H +++ +FL+F+ +++
Sbjct: 32 PCSVQHIYKFFDLMETHFLILSLAFLFFISLKLL 65
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,732,922
Number of Sequences: 28952
Number of extensions: 182664
Number of successful extensions: 590
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 578
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 590
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1334473344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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