Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_I24
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 221 7e-59
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 122 3e-29
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 122 6e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 105 4e-24
SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ... 29 0.44
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.1
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.1
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 4.1
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 5.5
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.2
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 221 bits (540), Expect = 7e-59
Identities = 101/152 (66%), Positives = 117/152 (76%)
Frame = +3
Query: 198 YHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS 377
YHG S+ Q ER+NVY+NEA+GGKYVPRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQS
Sbjct: 36 YHGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQS 95
Query: 378 GAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQXFQXXXXXXXXXXXXXXXXXXXKI 557
GAGN WAKGHYTEGAEL D+VLDVVR+EAE+CD LQ FQ KI
Sbjct: 96 GAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKI 155
Query: 558 XXXYPDRIMNTYSVVPSPKVSDTVVEPYNATL 653
YPDR+M T+SV P+PK SDTVVEPYNATL
Sbjct: 156 REEYPDRMMATFSVAPAPKSSDTVVEPYNATL 187
Score = 65.3 bits (152), Expect = 7e-12
Identities = 26/34 (76%), Positives = 29/34 (85%)
Frame = +2
Query: 92 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG 193
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAG 34
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 122 bits (295), Expect = 3e-29
Identities = 56/140 (40%), Positives = 82/140 (58%)
Frame = +3
Query: 234 NVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYT 413
+ +++E GKYVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT
Sbjct: 50 STFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYT 109
Query: 414 EGAELVDSVLDVVRKEAESCDCLQXFQXXXXXXXXXXXXXXXXXXXKIXXXYPDRIMNTY 593
G ELVD V D +R+ A++C LQ F ++ Y + +
Sbjct: 110 VGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQF 169
Query: 594 SVVPSPKVSDTVVEPYNATL 653
SV P+P+VS +VVEPYN+ L
Sbjct: 170 SVYPAPQVSTSVVEPYNSVL 189
Score = 44.4 bits (100), Expect = 1e-05
Identities = 19/34 (55%), Positives = 21/34 (61%)
Frame = +2
Query: 92 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG 193
MREI+ I GQ G QIG WE+ EHGI P G
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 122 bits (293), Expect = 6e-29
Identities = 56/151 (37%), Positives = 87/151 (57%)
Frame = +3
Query: 201 HGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSG 380
H ++ + +++E GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+
Sbjct: 43 HKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKED 102
Query: 381 AGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQXFQXXXXXXXXXXXXXXXXXXXKIX 560
A NN+A+GHYT G E++DSVL+ +R+ A++C LQ F ++
Sbjct: 103 ASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLN 162
Query: 561 XXYPDRIMNTYSVVPSPKVSDTVVEPYNATL 653
Y + +SV P+P+VS +VVEPYN+ L
Sbjct: 163 MEYGKKSNLQFSVYPAPQVSTSVVEPYNSVL 193
Score = 41.5 bits (93), Expect = 1e-04
Identities = 17/34 (50%), Positives = 21/34 (61%)
Frame = +2
Query: 92 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG 193
MRE++ + GQ G QIG WE+ EHGI P G
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG 34
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 105 bits (253), Expect = 4e-24
Identities = 49/147 (33%), Positives = 86/147 (58%), Gaps = 3/147 (2%)
Frame = +3
Query: 222 LERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNW 395
++R +V++ ++ +Y+PRAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNW
Sbjct: 45 VDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNW 104
Query: 396 AKGHYTEGAELVDSVLDVVRKEAESCDCLQXFQXXXXXXXXXXXXXXXXXXXKIXXXYPD 575
A G Y+ + + ++D++ +EA+ D L+ F ++ YP
Sbjct: 105 ANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPK 163
Query: 576 RIMNTYSVVP-SPKVSDTVVEPYNATL 653
+I+ TYSV P S VSD VV+PYN+ L
Sbjct: 164 KIIQTYSVFPNSQSVSDVVVQPYNSLL 190
Score = 54.4 bits (125), Expect = 1e-08
Identities = 22/35 (62%), Positives = 28/35 (80%)
Frame = +2
Query: 95 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGCL 199
REI+ +QAGQCGNQIG++FW+ + EHGI P G L
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTL 37
>SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit
Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 879
Score = 29.5 bits (63), Expect = 0.44
Identities = 14/40 (35%), Positives = 28/40 (70%)
Frame = -2
Query: 139 DLVAALTSLDMYDFPHFVLFM*TINYVNTKNLLRTLHSLS 20
D+++ TSL + + P L+M + + ++TK +L+TLH+L+
Sbjct: 123 DILSTGTSLRLANAPTSKLYMLSESPLSTKQILQTLHALN 162
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 327 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 428
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +3
Query: 396 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 485
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 4.1
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -2
Query: 469 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 332
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 348 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 479
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.2
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 385 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 290
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,289,719
Number of Sequences: 5004
Number of extensions: 39224
Number of successful extensions: 111
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 105
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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