BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_I18 (650 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC12C2.13c |fnx1|SPBC21D10.04c|MFS multidrug efflux transporte... 36 0.004 SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter |Schizosa... 33 0.036 SPBC1773.15 |||membrane transporter|Schizosaccharomyces pombe|ch... 29 0.58 SPBPB10D8.01 |||cysteine transporter |Schizosaccharomyces pombe|... 29 0.58 SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo... 27 2.3 SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 3.1 SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylg... 25 7.2 SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 25 7.2 >SPBC12C2.13c |fnx1|SPBC21D10.04c|MFS multidrug efflux transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 531 Score = 36.3 bits (80), Expect = 0.004 Identities = 19/89 (21%), Positives = 46/89 (51%) Frame = +2 Query: 380 EVTFSQYMTVSILCFVNLINYMDRLTIAGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFA 559 E++ +++ + L ++ +D +A + + +EF++ + A ++ T +++ F Sbjct: 25 ELSVNKWTILPALWVGGFLSALDMTIVASLYPVIGSEFKLMNN-ASYIVTAYLITNTAFQ 83 Query: 560 PIXGYLGDRYSRRVIMASGVALWSLTTFF 646 P+ G L D + RR + A ++L TF+ Sbjct: 84 PLYGRLSDIFGRRPTVVFANAAFTLGTFW 112 >SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 33.1 bits (72), Expect = 0.036 Identities = 21/84 (25%), Positives = 36/84 (42%), Gaps = 1/84 (1%) Frame = +2 Query: 398 YMTVSILCFVNLINYMDRLTIAGV-LNNVQTEFEIGDGKAGFLQTVFVVAYMLFAPIXGY 574 + +S C INY+DR +I L+ +Q + ++ + + VF Y++ Y Sbjct: 30 WFILSYCCVSYFINYLDRSSINNAYLSGMQEDLKMHGNELQDINVVFTCGYIIGQLPGSY 89 Query: 575 LGDRYSRRVIMASGVALWSLTTFF 646 R R+ + LW L T F Sbjct: 90 ALQRVPARLWFSVMNILWGLMTIF 113 >SPBC1773.15 |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 497 Score = 29.1 bits (62), Expect = 0.58 Identities = 13/75 (17%), Positives = 36/75 (48%), Gaps = 1/75 (1%) Frame = +2 Query: 413 ILCFVNLINYMDRLTI-AGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFAPIXGYLGDRY 589 +LC ++ YMD+ T + ++T+ + + ++ T F + +M+F+ L ++ Sbjct: 55 LLCLISAFQYMDKSTSNYSSIMGIRTDLNMVGNQYNWVGTSFYLGFMVFSLPLSTLLQKF 114 Query: 590 SRRVIMASGVALWSL 634 + ++ + W + Sbjct: 115 PLSKVTSAFIVAWGI 129 >SPBPB10D8.01 |||cysteine transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 552 Score = 29.1 bits (62), Expect = 0.58 Identities = 19/92 (20%), Positives = 47/92 (51%), Gaps = 3/92 (3%) Frame = +2 Query: 377 REVTFSQYMTVSILCFV-NLINYMDRLTIA-GVLNNVQTEFEIGDGKAGFLQTVFVVAYM 550 + + + Y TV ++ + +++ Y+D+ T++ + + + I + + T+F V ++ Sbjct: 79 KRLRWKLYFTVLVMLMILDMMLYIDKATLSYSSILGLFEDTHINSNQYNDINTLFYVGFV 138 Query: 551 LFAPIXGY-LGDRYSRRVIMASGVALWSLTTF 643 + I G+ L R+ +A+ ALW++ F Sbjct: 139 I-GQIPGHMLLQRFPVSKFVAASTALWTIIIF 169 >SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1826 Score = 27.1 bits (57), Expect = 2.3 Identities = 23/89 (25%), Positives = 38/89 (42%), Gaps = 2/89 (2%) Frame = +2 Query: 338 LLQEKQAXRHNXLREVTFSQYMTVSILCFVNLINYMDRLTIAGVLNNVQTEFEIGD--GK 511 +L+ R+N + + + VSIL +N++ I G N Q + + G Sbjct: 484 ILEAYFVPRNNPGAQPVIPRLIFVSILIALNIV---PAAFIFGFSNATQQHYRSREIVGY 540 Query: 512 AGFLQTVFVVAYMLFAPIXGYLGDRYSRR 598 F ++ VAY F P+ LG R+ R Sbjct: 541 VHFFFSIGCVAYQSFIPLPFLLGPRFKFR 569 >SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 577 Score = 26.6 bits (56), Expect = 3.1 Identities = 9/29 (31%), Positives = 15/29 (51%) Frame = +2 Query: 515 GFLQTVFVVAYMLFAPIXGYLGDRYSRRV 601 G + F + L P+ GY D+Y+ R+ Sbjct: 216 GIINVFFAIGSSLGGPVGGYFADQYTWRI 244 Score = 25.8 bits (54), Expect = 5.4 Identities = 18/98 (18%), Positives = 41/98 (41%) Frame = +2 Query: 350 KQAXRHNXLREVTFSQYMTVSILCFVNLINYMDRLTIAGVLNNVQTEFEIGDGKAGFLQT 529 + A N + V+ S+ + +L V + +D +A + EF + + T Sbjct: 75 QDAATTNTVSTVSVSKVLPALLLGVV--LAALDNTIVASTYTKIGAEFG-KFSQVSWTAT 131 Query: 530 VFVVAYMLFAPIXGYLGDRYSRRVIMASGVALWSLTTF 643 ++++ F P+ G D Y R+ + + ++ + F Sbjct: 132 AYMISCTAFQPLFGKFCDIYGRKKTLLAAYCVFGIGCF 169 >SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylglycerol ethanolaminesphotranferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 386 Score = 25.4 bits (53), Expect = 7.2 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 398 YMTVSILCFVNLINYMDRLTIAGVLNNVQTEF 493 Y V IL F L++ +D T GVL NVQTE+ Sbjct: 315 YWNVLILPF--LVDAVDAYTF-GVLKNVQTEY 343 >SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 993 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/52 (26%), Positives = 25/52 (48%) Frame = +2 Query: 239 VLETLVIMDPRGITPNTSNQQLVINGDNDSATALLQEKQAXRHNXLREVTFS 394 ++ T +++ P + PNT+ Q +I GDND+ H+ +T S Sbjct: 800 MIGTALLISP-ALEPNTTYIQGIIPGDNDTIWYDWYNHSVINHDYDENITMS 850 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,122,310 Number of Sequences: 5004 Number of extensions: 36917 Number of successful extensions: 88 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 87 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 88 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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