Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_I18
(650 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g46980.2 68416.m05102 transporter-related low similarity to b... 47 1e-05
At3g46980.1 68416.m05101 transporter-related low similarity to b... 47 1e-05
At4g00370.1 68417.m00051 sugar transporter family protein contai... 46 3e-05
At5g65687.1 68418.m08267 transporter-related low similarity to s... 44 1e-04
At2g38060.1 68415.m04672 transporter-related low similarity to v... 42 5e-04
At4g36790.1 68417.m05219 transporter-related low similarity to s... 39 0.003
At2g29650.2 68415.m03604 inorganic phosphate transporter, putati... 37 0.013
At2g29650.1 68415.m03603 inorganic phosphate transporter, putati... 37 0.013
At5g64500.1 68418.m08103 membrane protein-related contains weak ... 36 0.031
At5g20380.1 68418.m02424 transporter-related low similarity to v... 33 0.22
At3g47420.1 68416.m05156 glycerol-3-phosphate transporter, putat... 32 0.29
At2g22730.1 68415.m02694 transporter-related low similarity to s... 32 0.29
At2g16990.1 68415.m01959 expressed protein 30 1.5
At3g44940.1 68416.m04842 expressed protein 29 2.7
At3g43790.3 68416.m04680 transporter-related low similarity to S... 29 2.7
At3g43790.2 68416.m04679 transporter-related low similarity to S... 29 2.7
At3g43790.1 68416.m04678 transporter-related low similarity to S... 29 2.7
At4g25220.1 68417.m03629 transporter, putative similar to glycer... 29 3.5
At5g13740.1 68418.m01599 sugar transporter family protein contai... 28 4.7
At3g45330.1 68416.m04894 lectin protein kinase family protein co... 28 4.7
At1g72240.1 68414.m08352 expressed protein 28 4.7
At1g20860.1 68414.m02613 phosphate transporter family protein si... 28 4.7
At1g22830.1 68414.m02850 pentatricopeptide (PPR) repeat-containi... 27 8.2
>At3g46980.2 68416.m05102 transporter-related low similarity to
brain specific Na+-dependent inorganic phosphate
cotransporter from [Rattus norvegicus] GI:507415, [Homo
sapiens] GI:7328925, vesicular glutamate transporter 3
from [Rattus norvegicus] GI:21685382; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 469
Score = 46.8 bits (106), Expect = 1e-05
Identities = 26/87 (29%), Positives = 44/87 (50%)
Frame = +2
Query: 383 VTFSQYMTVSILCFVNLINYMDRLTIAGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFAP 562
+T + V++L + DR+ ++ + + +G +Q+ F+ Y++
Sbjct: 107 ITSERVKVVAMLALALALCNADRVVMSVAIVPLSLSRGWSKSFSGIVQSSFLWGYLISPI 166
Query: 563 IXGYLGDRYSRRVIMASGVALWSLTTF 643
G L DRY +V+MA GVALWSL TF
Sbjct: 167 AGGTLVDRYGGKVVMAWGVALWSLATF 193
>At3g46980.1 68416.m05101 transporter-related low similarity to
brain specific Na+-dependent inorganic phosphate
cotransporter from [Rattus norvegicus] GI:507415, [Homo
sapiens] GI:7328925, vesicular glutamate transporter 3
from [Rattus norvegicus] GI:21685382; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 533
Score = 46.8 bits (106), Expect = 1e-05
Identities = 26/87 (29%), Positives = 44/87 (50%)
Frame = +2
Query: 383 VTFSQYMTVSILCFVNLINYMDRLTIAGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFAP 562
+T + V++L + DR+ ++ + + +G +Q+ F+ Y++
Sbjct: 107 ITSERVKVVAMLALALALCNADRVVMSVAIVPLSLSRGWSKSFSGIVQSSFLWGYLISPI 166
Query: 563 IXGYLGDRYSRRVIMASGVALWSLTTF 643
G L DRY +V+MA GVALWSL TF
Sbjct: 167 AGGTLVDRYGGKVVMAWGVALWSLATF 193
>At4g00370.1 68417.m00051 sugar transporter family protein contains
Pfam profile PF00083: major facilitator superfamily
protein
Length = 541
Score = 45.6 bits (103), Expect = 3e-05
Identities = 28/113 (24%), Positives = 53/113 (46%), Gaps = 1/113 (0%)
Frame = +2
Query: 305 VINGDNDSATALLQEKQAXRHNXLREVTFSQYMTVSILCFVN-LINYMDRLTIAGVLNNV 481
V+ + SA A+L E + + F + + +LCF + L+ MDR+ ++ + +
Sbjct: 101 VVPSADGSAEAILVEGNLQNASPWWQ-QFPRRWVIVLLCFSSFLLCNMDRVNMSIAILPM 159
Query: 482 QTEFEIGDGKAGFLQTVFVVAYMLFAPIXGYLGDRYSRRVIMASGVALWSLTT 640
E+ G +Q+ F Y+L + G D++ +V++ GV WS T
Sbjct: 160 SQEYNWSSATVGLIQSSFFWGYLLTQILGGIWADKFGGKVVLGFGVVWWSFAT 212
>At5g65687.1 68418.m08267 transporter-related low similarity to
spinster membrane proteins from [Drosophila
melanogaster] GI:12003976, GI:12003972, GI:12003974,
GI:12003970; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 492
Score = 43.6 bits (98), Expect = 1e-04
Identities = 28/93 (30%), Positives = 49/93 (52%), Gaps = 17/93 (18%)
Frame = +2
Query: 407 VSILCFVNLINYMDRLTIA--------------GVLN---NVQTEFEIGDGKAGFLQTVF 535
V+ILC +NLINY+DR IA GV + +Q EF + + + G L + F
Sbjct: 30 VTILCIINLINYVDRGVIASNGVNGSSKVCDAKGVCSAGTGIQGEFNLTNFEDGLLSSAF 89
Query: 536 VVAYMLFAPIXGYLGDRYSRRVIMASGVALWSL 634
+V ++ +PI L R++ ++ G+ +W++
Sbjct: 90 MVGLLVASPIFAGLSKRFNPFKLIGVGLTVWTI 122
>At2g38060.1 68415.m04672 transporter-related low similarity to
vesicular glutamate transporter 3 [Homo sapiens]
GI:21213895, brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 512
Score = 41.5 bits (93), Expect = 5e-04
Identities = 24/80 (30%), Positives = 41/80 (51%)
Frame = +2
Query: 401 MTVSILCFVNLINYMDRLTIAGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFAPIXGYLG 580
+T ++C N DR+ ++ + + + G +Q+ F+ Y+ + I G L
Sbjct: 104 LTACMMCLCNA----DRVVMSVAVVPLADKLGWSSSFLGVVQSSFLWGYIFSSVIGGALV 159
Query: 581 DRYSRRVIMASGVALWSLTT 640
DRY + ++A GVALWSL T
Sbjct: 160 DRYGGKRVLAWGVALWSLAT 179
>At4g36790.1 68417.m05219 transporter-related low similarity to
spinster membrane proteins from [Drosophila
melanogaster] GI:12003976, GI:12003972, GI:12003974,
GI:12003970; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 489
Score = 38.7 bits (86), Expect = 0.003
Identities = 26/85 (30%), Positives = 39/85 (45%), Gaps = 4/85 (4%)
Frame = +2
Query: 398 YMTVSI-LCFVNLINYMDRLT---IAGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFAPI 565
++ VSI L +NL M+R + V V F G G+L V L +P+
Sbjct: 38 FLGVSISLILINLAAIMERADENLLPSVYKEVSEAFNAGPSDLGYLTFVRNFVQGLASPL 97
Query: 566 XGYLGDRYSRRVIMASGVALWSLTT 640
G L Y R +++A G W+L+T
Sbjct: 98 AGVLVITYDRPIVLAIGTFCWALST 122
>At2g29650.2 68415.m03604 inorganic phosphate transporter, putative
similar to brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 398
Score = 36.7 bits (81), Expect = 0.013
Identities = 21/85 (24%), Positives = 38/85 (44%), Gaps = 1/85 (1%)
Frame = +2
Query: 389 FSQYMTVSILCF-VNLINYMDRLTIAGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFAPI 565
F + + +LCF L+ MDR+ ++ + + E+ G +Q+ F Y+L
Sbjct: 99 FPKRWVIVLLCFSAFLLCNMDRVNMSIAILPMSAEYGWNPATVGLIQSSFFWGYLLTQIA 158
Query: 566 XGYLGDRYSRRVIMASGVALWSLTT 640
G D + ++ GV WS+ T
Sbjct: 159 GGIWADTVGGKRVLGFGVIWWSIAT 183
>At2g29650.1 68415.m03603 inorganic phosphate transporter, putative
similar to brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 512
Score = 36.7 bits (81), Expect = 0.013
Identities = 21/85 (24%), Positives = 38/85 (44%), Gaps = 1/85 (1%)
Frame = +2
Query: 389 FSQYMTVSILCF-VNLINYMDRLTIAGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFAPI 565
F + + +LCF L+ MDR+ ++ + + E+ G +Q+ F Y+L
Sbjct: 99 FPKRWVIVLLCFSAFLLCNMDRVNMSIAILPMSAEYGWNPATVGLIQSSFFWGYLLTQIA 158
Query: 566 XGYLGDRYSRRVIMASGVALWSLTT 640
G D + ++ GV WS+ T
Sbjct: 159 GGIWADTVGGKRVLGFGVIWWSIAT 183
>At5g64500.1 68418.m08103 membrane protein-related contains weak
similarity to spinster type IV (GI:12003976) [Drosophila
melanogaster]
Length = 484
Score = 35.5 bits (78), Expect = 0.031
Identities = 25/99 (25%), Positives = 48/99 (48%), Gaps = 17/99 (17%)
Frame = +2
Query: 389 FSQYMTVSILCFVNLINYMDRLTIA--------------GVLNN---VQTEFEIGDGKAG 517
F+ + + C VNLINY+DR IA G ++ +Q +F + + + G
Sbjct: 33 FTPKKLLFVFCVVNLINYIDRGAIASNGINGSRGSCTSSGTCSSGSGIQGDFNLSNFEDG 92
Query: 518 FLQTVFVVAYMLFAPIXGYLGDRYSRRVIMASGVALWSL 634
L + F+V ++ +PI L + ++ G+++W+L
Sbjct: 93 VLSSAFMVGLLVASPIFASLAKSVNPFRLIGVGLSIWTL 131
>At5g20380.1 68418.m02424 transporter-related low similarity to
vesicular glutamate transporter 3 [Rattus norvegicus]
GI:21685382
Length = 517
Score = 32.7 bits (71), Expect = 0.22
Identities = 19/82 (23%), Positives = 35/82 (42%)
Frame = +2
Query: 395 QYMTVSILCFVNLINYMDRLTIAGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFAPIXGY 574
+Y + +I MD++ ++ + + +F AG +Q+ F Y L G+
Sbjct: 98 RYKLIGATSLAFVICNMDKVNLSIAIIPMSHQFGWSSSVAGLVQSSFFWGYALSQLPGGW 157
Query: 575 LGDRYSRRVIMASGVALWSLTT 640
L + R ++ GV WS T
Sbjct: 158 LSKIFGGRKVLEIGVFTWSFAT 179
>At3g47420.1 68416.m05156 glycerol-3-phosphate transporter, putative
/ glycerol 3-phosphate permease, putative similar to
cAMP inducible 2 protein [Mus musculus] GI:4580997,
glycerol-3-phosphate transporter (glycerol 3-phosphate
permease) [Homo sapiens] GI:7543982; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 523
Score = 32.3 bits (70), Expect = 0.29
Identities = 22/78 (28%), Positives = 35/78 (44%), Gaps = 1/78 (1%)
Frame = +2
Query: 416 LCFVNLINYMDRLTIAGVLNNVQTEFE-IGDGKAGFLQTVFVVAYMLFAPIXGYLGDRYS 592
L F L+ Y + +++ E E + D AG L T+F V ++ + GY+ DR
Sbjct: 310 LFFAKLVAYTFLYWLPFYVSHTAIEGEYLSDETAGNLSTMFDVGGVVGGIMAGYISDRIG 369
Query: 593 RRVIMASGVALWSLTTFF 646
R I A+ S+ F
Sbjct: 370 ARAITAASFMYCSIPALF 387
>At2g22730.1 68415.m02694 transporter-related low similarity to
spinster membrane proteins from [Drosophila
melanogaster] GI:12003974, GI:12003976, GI:12003972,
GI:12003970; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 510
Score = 32.3 bits (70), Expect = 0.29
Identities = 23/91 (25%), Positives = 41/91 (45%), Gaps = 17/91 (18%)
Frame = +2
Query: 413 ILCFVNLINYMDRLTIAG-----------------VLNNVQTEFEIGDGKAGFLQTVFVV 541
I C +NL+NYMDR IA + +Q F + + + G L + F+V
Sbjct: 54 IFCIINLLNYMDRGAIASNGVNGSTRSCNDKGKCTLATGIQGHFNLSNFEDGVLSSSFMV 113
Query: 542 AYMLFAPIXGYLGDRYSRRVIMASGVALWSL 634
++ +PI L R ++ G+ +W++
Sbjct: 114 GLLIASPIFASLAKR-----LIGVGLTVWTI 139
>At2g16990.1 68415.m01959 expressed protein
Length = 456
Score = 29.9 bits (64), Expect = 1.5
Identities = 15/58 (25%), Positives = 25/58 (43%)
Frame = +2
Query: 452 LTIAGVLNNVQTEFEIGDGKAGFLQTVFVVAYMLFAPIXGYLGDRYSRRVIMASGVAL 625
+T+A V + + GF Q + M+ P+ G L DRY + I+ + L
Sbjct: 32 VTVAAVCSGPDDSCSLAVYLTGFQQVAIGMGTMIMMPVIGNLSDRYGIKTILTLPMCL 89
>At3g44940.1 68416.m04842 expressed protein
Length = 250
Score = 29.1 bits (62), Expect = 2.7
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = +2
Query: 257 IMDPRGITPNTSNQQLVINGDNDSATAL 340
IM P I P T NQQ+ ++GD AT L
Sbjct: 135 IMSPSVIDPVTMNQQIEVSGDEMLATLL 162
>At3g43790.3 68416.m04680 transporter-related low similarity to
SP|P39843 Multidrug resistance protein 2
(Multidrug-efflux transporter 2) {Bacillus subtilis};
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 484
Score = 29.1 bits (62), Expect = 2.7
Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +2
Query: 512 AGFLQTVFVVAYMLFAPIXGYLGDRYSRRVIMASGV-ALWSLTTFFG 649
AGF+ + F++ L + G L DRY R+ I+ G ++ T FG
Sbjct: 78 AGFVGSSFMIGRALTSIFWGKLADRYGRKPIILIGTFSVIIFNTLFG 124
>At3g43790.2 68416.m04679 transporter-related low similarity to
SP|P39843 Multidrug resistance protein 2
(Multidrug-efflux transporter 2) {Bacillus subtilis};
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 484
Score = 29.1 bits (62), Expect = 2.7
Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +2
Query: 512 AGFLQTVFVVAYMLFAPIXGYLGDRYSRRVIMASGV-ALWSLTTFFG 649
AGF+ + F++ L + G L DRY R+ I+ G ++ T FG
Sbjct: 78 AGFVGSSFMIGRALTSIFWGKLADRYGRKPIILIGTFSVIIFNTLFG 124
>At3g43790.1 68416.m04678 transporter-related low similarity to
SP|P39843 Multidrug resistance protein 2
(Multidrug-efflux transporter 2) {Bacillus subtilis};
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 478
Score = 29.1 bits (62), Expect = 2.7
Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +2
Query: 512 AGFLQTVFVVAYMLFAPIXGYLGDRYSRRVIMASGV-ALWSLTTFFG 649
AGF+ + F++ L + G L DRY R+ I+ G ++ T FG
Sbjct: 78 AGFVGSSFMIGRALTSIFWGKLADRYGRKPIILIGTFSVIIFNTLFG 124
>At4g25220.1 68417.m03629 transporter, putative similar to
glycerol-3-phosphate transporter (glycerol 3-phosphate
permease) [Homo sapiens] GI:7543982
Length = 504
Score = 28.7 bits (61), Expect = 3.5
Identities = 13/50 (26%), Positives = 26/50 (52%)
Frame = +2
Query: 497 IGDGKAGFLQTVFVVAYMLFAPIXGYLGDRYSRRVIMASGVALWSLTTFF 646
+ + +G L T+F V ++ + GY+ D+ + R I A+G ++ F
Sbjct: 318 LSEETSGNLSTIFDVGGVVGGVLAGYISDQLNGRAITAAGFMYLAIPALF 367
>At5g13740.1 68418.m01599 sugar transporter family protein contains
Pfam profile PF00083: major facilitator superfamily
protein
Length = 486
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/57 (29%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +2
Query: 482 QTEFEIGDGKAGFLQTVFVVAYMLFAPIXGYLGDRYSRR-VIMASGVALWSLTTFFG 649
+TE +IG AGF+ F++ L + G + DRY R+ +I+ +++ FG
Sbjct: 71 KTEKDIGF-YAGFVGCSFMLGRALTSVFWGIVADRYGRKPIILLGTISIAIFNALFG 126
>At3g45330.1 68416.m04894 lectin protein kinase family protein
contains Serine/Threonine protein kinases active-site
signature, Prosite:PS00108; contains Pfam profiles
PF00069: Protein kinase domain, PF00139: Legume lectins
beta domain, PF00138: Legume lectins alpha domain
Length = 682
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 3/41 (7%)
Frame = +2
Query: 413 ILCFVNLINYMDRLTIAGVLNNV---QTEFEIGDGKAGFLQ 526
++CFVNLI++ + ++ + N QT + DG A FLQ
Sbjct: 13 LICFVNLISFSSQQDLSFIYNGFNQDQTNLNL-DGSAKFLQ 52
>At1g72240.1 68414.m08352 expressed protein
Length = 133
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = -3
Query: 495 SNSVWTLFKTPAIVNLSM*LMRLTKHNIETVIYCEKVTSRXXLCLXACFSCRSAVAESLS 316
S + +L T + LS+ TK +ET YC ++ + +C+S S+V+ S
Sbjct: 46 SPGICSLCLTERLSKLSLEYYDYTKKAVETATYCGSTSTSSSSSVSSCYS-SSSVSSCSS 104
Query: 315 P 313
P
Sbjct: 105 P 105
>At1g20860.1 68414.m02613 phosphate transporter family protein
similar to phosphate transporter [Catharanthus roseus]
GI:2208908, inorganic phosphate transporter 1 [Solanum
tuberosum] GI:1420871; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 534
Score = 28.3 bits (60), Expect = 4.7
Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 4/70 (5%)
Frame = +2
Query: 446 DRLTIAGVLNNVQTEFEIGDG-KAGFLQTVFVVAYMLFAP---IXGYLGDRYSRRVIMAS 613
D IA V+ + + GD L T + +A + A + GYLGDR RR +
Sbjct: 35 DLFCIAPVMKMISHVYYNGDSINTAVLSTSYAIALLGTATGQLVFGYLGDRVGRRRVYGL 94
Query: 614 GVALWSLTTF 643
+ + L++F
Sbjct: 95 CLIIMILSSF 104
>At1g22830.1 68414.m02850 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat. Gene
continues on the 3' end of BAC F19G10 gb|AF000657 gene
F19G10.21
Length = 703
Score = 27.5 bits (58), Expect = 8.2
Identities = 20/63 (31%), Positives = 31/63 (49%)
Frame = -1
Query: 305 LTVGSKCSVLYHEGP*LQEFQALSNSTRKRPLSGIFINEMSSNLWLKSILYIKSILXTNN 126
+T+ S+CS L H Q+ +A S ST +SG NE S ++ +K +L +
Sbjct: 364 ITMYSRCSDLRHAFIVFQQVEANSLSTWNSIISGFAYNERSE----ETSFLLKEMLLSGF 419
Query: 125 HRN 117
H N
Sbjct: 420 HPN 422
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,647,351
Number of Sequences: 28952
Number of extensions: 177214
Number of successful extensions: 435
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 417
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 435
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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