BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_I08
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha... 198 3e-51
At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha... 195 2e-50
At5g57140.1 68418.m08530 calcineurin-like phosphoesterase family... 27 8.2
At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containi... 27 8.2
At4g18240.1 68417.m02709 starch synthase-related protein contain... 27 8.2
At1g40129.1 68414.m04766 hypothetical protein 27 8.2
>At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial, putative / succinyl-CoA
synthetase, alpha chain, putative / SCS-alpha, putative
identical to SP|P53586 Succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial precursor (EC 6.2.1.4)
(Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
{Arabidopsis thaliana}; strong similarity to SP|P13086
Succinyl-CoA ligase [GDP-forming] alpha-chain,
mitochondrial precursor {Rattus norvegicus}; contains
Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding
domain
Length = 347
Score = 198 bits (482), Expect = 3e-51
Identities = 93/148 (62%), Positives = 112/148 (75%)
Frame = +2
Query: 209 CSGLSLESRATFHSQQALDYGTKVVGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVI 388
C G++ ++ TFH++QA++YGTK+V GV+PKK GTEHLG PVF +V EAKA T A ASVI
Sbjct: 62 CQGITGKN-GTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNSVAEAKADTKANASVI 120
Query: 389 YVPPPGXXXXXXXXXXXXMPLIVCITEGVPQHDMVRVKHALLRQNKSXLVGPNCPGIIAP 568
YVP P + LIVCITEG+PQHDMVRVKHAL Q+K+ L+GPNCPGII P
Sbjct: 121 YVPAPFAAAAIMEGIEAELDLIVCITEGIPQHDMVRVKHALNSQSKTRLIGPNCPGIIKP 180
Query: 569 XKCKIGIMPAAVHKRGCIGVVSRSGTLT 652
+CKIGIMP +HK G IG+VSRSGTLT
Sbjct: 181 GECKIGIMPGYIHKPGKIGIVSRSGTLT 208
>At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial, putative / succinyl-CoA
synthetase, alpha chain, putative / SCS-alpha, putative
similar to SP|P36967 Succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial precursor (EC 6.2.1.4)
(Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
{Dictyostelium discoideum}; contains Pfam profiles
PF00549: CoA-ligase, PF02629: CoA binding domain
Length = 341
Score = 195 bits (475), Expect = 2e-50
Identities = 93/148 (62%), Positives = 111/148 (75%)
Frame = +2
Query: 209 CSGLSLESRATFHSQQALDYGTKVVGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVI 388
C G++ ++ TFH++QA++YGTK+V GV+PKK GTEHLG PVF TV EAKA T A ASVI
Sbjct: 57 CQGITGKN-GTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNTVAEAKAETKANASVI 115
Query: 389 YVPPPGXXXXXXXXXXXXMPLIVCITEGVPQHDMVRVKHALLRQNKSXLVGPNCPGIIAP 568
YVP P + LIVCITEG+PQHDMVRVK AL Q+K+ L+GPNCPGII P
Sbjct: 116 YVPAPFAAAAIMEGLAAELDLIVCITEGIPQHDMVRVKAALNSQSKTRLIGPNCPGIIKP 175
Query: 569 XKCKIGIMPAAVHKRGCIGVVSRSGTLT 652
+CKIGIMP +HK G IG+VSRSGTLT
Sbjct: 176 GECKIGIMPGYIHKPGKIGIVSRSGTLT 203
>At5g57140.1 68418.m08530 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 Calcineurin-like
phosphoesterase
Length = 397
Score = 27.5 bits (58), Expect = 8.2
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = -2
Query: 95 PDWHSHCRIIECKSTKGKN 39
P+WH R+IE K KG++
Sbjct: 343 PNWHRRARVIEAKLGKGRD 361
>At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 534
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -2
Query: 107 LRKNPDWHSHCRIIECKSTKG 45
LR PD SHCR+IE + G
Sbjct: 97 LRFEPDLKSHCRVIEVAAESG 117
>At4g18240.1 68417.m02709 starch synthase-related protein contains
similarity to starch synthase GI:4582783 from [Vigna
unguiculata]
Length = 1040
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +2
Query: 446 PLIVCITEGVPQHDMVRVKHALLR 517
PL+ CIT VPQ + ++HA+ R
Sbjct: 842 PLVGCITRLVPQKGVHLIRHAIYR 865
>At1g40129.1 68414.m04766 hypothetical protein
Length = 567
Score = 27.5 bits (58), Expect = 8.2
Identities = 12/29 (41%), Positives = 15/29 (51%), Gaps = 1/29 (3%)
Frame = -1
Query: 324 PRCSVPAFFGDTPPTTLVP-*SRACWLWK 241
PR FF D PP +P +R C LW+
Sbjct: 329 PRAECNIFFKDKPPDATLPFKTRGCTLWR 357
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,933,362
Number of Sequences: 28952
Number of extensions: 273187
Number of successful extensions: 614
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 604
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 614
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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