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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_I08
         (654 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha...   198   3e-51
At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha...   195   2e-50
At5g57140.1 68418.m08530 calcineurin-like phosphoesterase family...    27   8.2  
At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containi...    27   8.2  
At4g18240.1 68417.m02709 starch synthase-related protein contain...    27   8.2  
At1g40129.1 68414.m04766 hypothetical protein                          27   8.2  

>At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial, putative / succinyl-CoA
           synthetase, alpha chain, putative / SCS-alpha, putative
           identical to SP|P53586 Succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial precursor (EC 6.2.1.4)
           (Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
           {Arabidopsis thaliana}; strong similarity to SP|P13086
           Succinyl-CoA ligase [GDP-forming] alpha-chain,
           mitochondrial precursor {Rattus norvegicus}; contains
           Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding
           domain
          Length = 347

 Score =  198 bits (482), Expect = 3e-51
 Identities = 93/148 (62%), Positives = 112/148 (75%)
 Frame = +2

Query: 209 CSGLSLESRATFHSQQALDYGTKVVGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVI 388
           C G++ ++  TFH++QA++YGTK+V GV+PKK GTEHLG PVF +V EAKA T A ASVI
Sbjct: 62  CQGITGKN-GTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNSVAEAKADTKANASVI 120

Query: 389 YVPPPGXXXXXXXXXXXXMPLIVCITEGVPQHDMVRVKHALLRQNKSXLVGPNCPGIIAP 568
           YVP P             + LIVCITEG+PQHDMVRVKHAL  Q+K+ L+GPNCPGII P
Sbjct: 121 YVPAPFAAAAIMEGIEAELDLIVCITEGIPQHDMVRVKHALNSQSKTRLIGPNCPGIIKP 180

Query: 569 XKCKIGIMPAAVHKRGCIGVVSRSGTLT 652
            +CKIGIMP  +HK G IG+VSRSGTLT
Sbjct: 181 GECKIGIMPGYIHKPGKIGIVSRSGTLT 208


>At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial, putative / succinyl-CoA
           synthetase, alpha chain, putative / SCS-alpha, putative
           similar to SP|P36967 Succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial precursor (EC 6.2.1.4)
           (Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
           {Dictyostelium discoideum}; contains Pfam profiles
           PF00549: CoA-ligase, PF02629: CoA binding domain
          Length = 341

 Score =  195 bits (475), Expect = 2e-50
 Identities = 93/148 (62%), Positives = 111/148 (75%)
 Frame = +2

Query: 209 CSGLSLESRATFHSQQALDYGTKVVGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVI 388
           C G++ ++  TFH++QA++YGTK+V GV+PKK GTEHLG PVF TV EAKA T A ASVI
Sbjct: 57  CQGITGKN-GTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNTVAEAKAETKANASVI 115

Query: 389 YVPPPGXXXXXXXXXXXXMPLIVCITEGVPQHDMVRVKHALLRQNKSXLVGPNCPGIIAP 568
           YVP P             + LIVCITEG+PQHDMVRVK AL  Q+K+ L+GPNCPGII P
Sbjct: 116 YVPAPFAAAAIMEGLAAELDLIVCITEGIPQHDMVRVKAALNSQSKTRLIGPNCPGIIKP 175

Query: 569 XKCKIGIMPAAVHKRGCIGVVSRSGTLT 652
            +CKIGIMP  +HK G IG+VSRSGTLT
Sbjct: 176 GECKIGIMPGYIHKPGKIGIVSRSGTLT 203


>At5g57140.1 68418.m08530 calcineurin-like phosphoesterase family
           protein contains Pfam profile: PF00149 Calcineurin-like
           phosphoesterase
          Length = 397

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = -2

Query: 95  PDWHSHCRIIECKSTKGKN 39
           P+WH   R+IE K  KG++
Sbjct: 343 PNWHRRARVIEAKLGKGRD 361


>At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 534

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 11/21 (52%), Positives = 13/21 (61%)
 Frame = -2

Query: 107 LRKNPDWHSHCRIIECKSTKG 45
           LR  PD  SHCR+IE  +  G
Sbjct: 97  LRFEPDLKSHCRVIEVAAESG 117


>At4g18240.1 68417.m02709 starch synthase-related protein contains
           similarity to starch synthase GI:4582783 from [Vigna
           unguiculata]
          Length = 1040

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 11/24 (45%), Positives = 16/24 (66%)
 Frame = +2

Query: 446 PLIVCITEGVPQHDMVRVKHALLR 517
           PL+ CIT  VPQ  +  ++HA+ R
Sbjct: 842 PLVGCITRLVPQKGVHLIRHAIYR 865


>At1g40129.1 68414.m04766 hypothetical protein
          Length = 567

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 12/29 (41%), Positives = 15/29 (51%), Gaps = 1/29 (3%)
 Frame = -1

Query: 324 PRCSVPAFFGDTPPTTLVP-*SRACWLWK 241
           PR     FF D PP   +P  +R C LW+
Sbjct: 329 PRAECNIFFKDKPPDATLPFKTRGCTLWR 357


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,933,362
Number of Sequences: 28952
Number of extensions: 273187
Number of successful extensions: 614
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 604
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 614
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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