Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_I05
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 219 1e-57
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 214 4e-56
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 214 4e-56
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 213 7e-56
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 210 5e-55
At2g03270.1 68415.m00280 DNA-binding protein, putative similar t... 28 4.7
At3g43300.1 68416.m04570 guanine nucleotide exchange family prot... 28 6.2
At1g54960.1 68414.m06277 NPK1-related protein kinase, putative (... 28 6.2
>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 2 (Methionine
adenosyltransferase 2, AdoMet synthetase 2)
[Catharanthus roseus] SWISS-PROT:Q96552
Length = 393
Score = 219 bits (535), Expect = 1e-57
Identities = 105/170 (61%), Positives = 128/170 (75%), Gaps = 2/170 (1%)
Frame = +1
Query: 148 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 327
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV++ GEIT+KANV
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63
Query: 328 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHXN--RNDEEVGAGDQ 501
DY+++VR+T + IG+ + G D C V++ ++QQSP+IA GVH + + EEVGAGDQ
Sbjct: 64 DYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEVGAGDQ 123
Query: 502 XLMFGYATDXTXXCMPLTVVLAHKLNXKIAXLXRNGEFWWARPDSKTQVT 651
MFGYATD T MPLT VLA KL K+ + +NG W RPD KTQVT
Sbjct: 124 GHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVT 173
>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 214 bits (523), Expect = 4e-56
Identities = 102/170 (60%), Positives = 126/170 (74%), Gaps = 2/170 (1%)
Frame = +1
Query: 148 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 327
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ GEIT+KA V
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63
Query: 328 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHXN--RNDEEVGAGDQ 501
DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + + EE+GAGDQ
Sbjct: 64 DYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQ 123
Query: 502 XLMFGYATDXTXXCMPLTVVLAHKLNXKIAXLXRNGEFWWARPDSKTQVT 651
MFGYATD T MPL+ VLA KL ++ + +NG W RPD KTQVT
Sbjct: 124 GHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVT 173
>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 214 bits (523), Expect = 4e-56
Identities = 102/170 (60%), Positives = 126/170 (74%), Gaps = 2/170 (1%)
Frame = +1
Query: 148 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 327
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ GEIT+KA V
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63
Query: 328 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHXN--RNDEEVGAGDQ 501
DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + + EE+GAGDQ
Sbjct: 64 DYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQ 123
Query: 502 XLMFGYATDXTXXCMPLTVVLAHKLNXKIAXLXRNGEFWWARPDSKTQVT 651
MFGYATD T MPL+ VLA KL ++ + +NG W RPD KTQVT
Sbjct: 124 GHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVT 173
>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
identical to S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2, AdoMet synthetase 2)
[Arabidopsis thaliana] SWISS-PROT:P17562
Length = 393
Score = 213 bits (521), Expect = 7e-56
Identities = 99/170 (58%), Positives = 126/170 (74%), Gaps = 2/170 (1%)
Frame = +1
Query: 148 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 327
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ GEIT+KA +
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATI 63
Query: 328 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHXN--RNDEEVGAGDQ 501
DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + + E++GAGDQ
Sbjct: 64 DYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQ 123
Query: 502 XLMFGYATDXTXXCMPLTVVLAHKLNXKIAXLXRNGEFWWARPDSKTQVT 651
MFGYATD T MPL+ VLA K+ ++ + +NG W RPD KTQVT
Sbjct: 124 GHMFGYATDETPELMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVT 173
>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 3 (Methionine
adenosyltransferase 3, AdoMet synthetase 3)
[Lycopersicon esculentum] SWISS-PROT:P43282
Length = 390
Score = 210 bits (514), Expect = 5e-55
Identities = 101/170 (59%), Positives = 125/170 (73%), Gaps = 2/170 (1%)
Frame = +1
Query: 148 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 327
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV++ GEIT+ A V
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTAAKV 63
Query: 328 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHXN--RNDEEVGAGDQ 501
DY+K+VR T + IG+ + G D C+V++ ++QQSP+IA GVH + + E++GAGDQ
Sbjct: 64 DYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKKPEDIGAGDQ 123
Query: 502 XLMFGYATDXTXXCMPLTVVLAHKLNXKIAXLXRNGEFWWARPDSKTQVT 651
MFGYATD T MPLT VLA KL K+ + +N W RPD KTQVT
Sbjct: 124 GHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVT 173
>At2g03270.1 68415.m00280 DNA-binding protein, putative similar to
Swiss-Prot:Q60560 DNA-binding protein SMUBP-2
(Immunoglobulin MU binding protein 2) (SMUBP-2) (Insulin
II gene enhancer-binding protein)(RIPE3B-binding complex
3B2 P110 subunit) (RIP-1)[Mesocricetus auratus];
identical to putative helicase (atpc-2 gene) cDNA
NCBI_gi:11191230
Length = 639
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/70 (18%), Positives = 40/70 (57%)
Frame = +1
Query: 178 GHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETV 357
GHP ++ Q+ D+ LDA + + ++ +A + + M L G++ + + ++++++ +
Sbjct: 264 GHPARLLPQVLDSALDAQVLKGDNSGLAND--IRKEMKALNGKLLKAKDKNTRRLIQKEL 321
Query: 358 KHIGYDDSSK 387
+ +G ++ +
Sbjct: 322 RTLGKEERKR 331
>At3g43300.1 68416.m04570 guanine nucleotide exchange family protein
similar to SP|Q9Y6D5 Brefeldin A-inhibited guanine
nucleotide-exchange protein 2 {Homo sapiens}; contains
Pfam profile PF01369: Sec7 domain
Length = 1756
Score = 27.9 bits (59), Expect = 6.2
Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Frame = +1
Query: 241 DPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRE---TVKHIGYDDSSKGFDYKTCS 411
DP+ +A +T + G L C K+ VD++K+ RE + ++ D +S G +T S
Sbjct: 523 DPNPAMASQTASVKGSSLQCLVNVLKSLVDWEKIRREAENSTRNANEDSASTGEPIETKS 582
>At1g54960.1 68414.m06277 NPK1-related protein kinase, putative
(ANP2) similar to protein kinase [Nicotiana tabacum]
gi|456309|dbj|BAA05648; identical to cDNA NPK1-related
protein kinase 2, partial cds GI:2342424
Length = 596
Score = 27.9 bits (59), Expect = 6.2
Identities = 13/49 (26%), Positives = 25/49 (51%)
Frame = +1
Query: 232 LNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDD 378
L+ + DAK +C+T+++ +L C + N + + V V H Y +
Sbjct: 431 LDNNTDAKKSCDTMSEISDILKCKFDENSGNGETETKVSMEVDHPSYSE 479
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,746,817
Number of Sequences: 28952
Number of extensions: 308423
Number of successful extensions: 718
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 696
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 713
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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