Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_I01
(493 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30575.1 68415.m03725 glycosyl transferase family 8 protein c... 31 0.32
At1g06420.1 68414.m00679 expressed protein ; expression supporte... 31 0.32
At1g33040.1 68414.m04068 nascent polypeptide-associated complex ... 31 0.42
At5g33220.1 68418.m03924 hypothetical protein contains Pfam prof... 30 0.97
At2g43690.1 68415.m05431 lectin protein kinase, putative similar... 29 1.7
At1g63980.1 68414.m07247 D111/G-patch domain-containing protein ... 29 1.7
At1g11510.1 68414.m01322 DNA-binding storekeeper protein-related... 29 1.7
At4g33500.1 68417.m04758 protein phosphatase 2C-related / PP2C-r... 29 2.2
At3g58840.1 68416.m06558 expressed protein 29 2.2
At4g02510.1 68417.m00343 chloroplast outer membrane protein, put... 28 3.0
At2g16920.1 68415.m01949 ubiquitin-conjugating enzyme family pro... 28 3.0
At2g03140.1 68415.m00267 CAAX amino terminal protease family pro... 28 3.0
At3g62360.1 68416.m07005 expressed protein 28 3.9
At1g65010.1 68414.m07368 expressed protein similar to endosome-a... 27 5.2
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 27 5.2
At5g57120.1 68418.m07132 expressed protein weak similarity to SP... 27 6.9
At5g46600.1 68418.m05737 expressed protein contains Pfam profile... 27 6.9
At3g52860.1 68416.m05825 expressed protein 27 6.9
At3g14900.1 68416.m01884 expressed protein 27 6.9
At4g14200.1 68417.m02191 expressed protein 27 9.1
At1g52980.1 68414.m05995 GTP-binding family protein contains Pfa... 27 9.1
>At2g30575.1 68415.m03725 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 610
Score = 31.5 bits (68), Expect = 0.32
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = +3
Query: 234 DAGDLKKSKKVLQDIEKDNEXVEMSSDDENVVVDSEGGKKRVFNTKTLKDQN 389
D LK+ K++LQD + ++ + SSD N V S G ++ F ++ K N
Sbjct: 65 DEEGLKEPKRILQDRDFNSVVLSNSSDKSNDTVQSNEGDQKNFLSEVDKGNN 116
>At1g06420.1 68414.m00679 expressed protein ; expression supported
by MPSS
Length = 221
Score = 31.5 bits (68), Expect = 0.32
Identities = 19/62 (30%), Positives = 30/62 (48%)
Frame = +3
Query: 87 SRWKRKCRAIKRERYAVKELXRLKKMLGVKDEEKPAGSEVMESEQVIFLDAGDLKKSKKV 266
+RWKR +K+E V + K GV EK + V E+ + +F G+ +K+V
Sbjct: 95 NRWKRDQVCVKKEEIKVSGIVSNKDEDGVDSREKKKKNPVKENTRRVFKSKGE-NAAKEV 153
Query: 267 LQ 272
Q
Sbjct: 154 TQ 155
>At1g33040.1 68414.m04068 nascent polypeptide-associated complex
(NAC) domain-containing protein similar to alpha-NAC,
non-muscle form [Mus musculus] GI:1666690; contains Pfam
profile PF01849: NAC domain
Length = 209
Score = 31.1 bits (67), Expect = 0.42
Identities = 15/44 (34%), Positives = 26/44 (59%)
Frame = +3
Query: 249 KKSKKVLQDIEKDNEXVEMSSDDENVVVDSEGGKKRVFNTKTLK 380
K+ V++D+ KD E + DDE+V V+ EGG + +++ K
Sbjct: 24 KEDDVVVEDV-KDGEEEDDDEDDEDVEVEGEGGNENAKQSRSEK 66
>At5g33220.1 68418.m03924 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 587
Score = 29.9 bits (64), Expect = 0.97
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = +3
Query: 177 DEEKPAGSEVMESEQVIFLDAGDLKKSKKVLQDIEKDNEXVEMSSDDEN 323
+EEK + V++ + D GD + +QD + DN M DD+N
Sbjct: 138 EEEKETTNAVVQHDDAANKDDGDDDNLSQNMQDGDDDNLPQNMQDDDDN 186
>At2g43690.1 68415.m05431 lectin protein kinase, putative similar to
receptor-like kinase LECRK1 [Arabidopsis thaliana]
gi|2150023|gb|AAB58725
Length = 664
Score = 29.1 bits (62), Expect = 1.7
Identities = 20/61 (32%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = +3
Query: 225 IFLDAGDLKKSKKVLQ---DIEKDNEXVEMSSDDENVVVDSEGGKKRVFNTKTLKDQNGQ 395
IF D D S ++ DI KD+E ++ DD +V ++ G + V DQNGQ
Sbjct: 114 IFNDTNDGNSSNHIIAVELDIHKDDEFGDI--DDNHVGININGMRSIVSAPAGYYDQNGQ 171
Query: 396 Y 398
+
Sbjct: 172 F 172
>At1g63980.1 68414.m07247 D111/G-patch domain-containing protein
contains Pfam profile PF01585: G-patch domain
Length = 391
Score = 29.1 bits (62), Expect = 1.7
Identities = 20/69 (28%), Positives = 31/69 (44%), Gaps = 2/69 (2%)
Frame = +3
Query: 156 KKMLGVKDEEKPAGSEVMESEQVIF--LDAGDLKKSKKVLQDIEKDNEXVEMSSDDENVV 329
K+ LG+KD K E ++ F D D +D E+D + E DD++ V
Sbjct: 239 KQGLGIKDRPKKIAGVRYEGKKTSFDNSDDDDDDDDDDDEEDEEEDEDESEADDDDKDSV 298
Query: 330 VDSEGGKKR 356
++S KR
Sbjct: 299 IESSLPAKR 307
>At1g11510.1 68414.m01322 DNA-binding storekeeper protein-related
similar to storekeeper protein GI:14268476 [Solanum
tuberosum]
Length = 352
Score = 29.1 bits (62), Expect = 1.7
Identities = 17/59 (28%), Positives = 31/59 (52%)
Frame = +3
Query: 177 DEEKPAGSEVMESEQVIFLDAGDLKKSKKVLQDIEKDNEXVEMSSDDENVVVDSEGGKK 353
DEE + SE+ + + + + D+K++KKV + EK + D+ V +D + KK
Sbjct: 59 DEETDSDSELEKKKDQVVTNPVDVKRAKKV-KTSEKSGAKRSLEVDEAAVSMDVKRAKK 116
>At4g33500.1 68417.m04758 protein phosphatase 2C-related /
PP2C-related YHR077c (NMD2,IFS1) protein -Saccharomyces
cerevisiae,PID:g555939
Length = 724
Score = 28.7 bits (61), Expect = 2.2
Identities = 21/78 (26%), Positives = 32/78 (41%)
Frame = +3
Query: 171 VKDEEKPAGSEVMESEQVIFLDAGDLKKSKKVLQDIEKDNEXVEMSSDDENVVVDSEGGK 350
+ EK +E E V +K V D+E D E V +++DE V D+
Sbjct: 379 IDTSEKNPMETFVEPEAVHSSVDESTEKLVVVTSDVENDGENVASTTEDEITVRDTITDS 438
Query: 351 KRVFNTKTLKDQNGQYPV 404
+ N K ++ Q PV
Sbjct: 439 GSISNNDDTKVEDLQLPV 456
>At3g58840.1 68416.m06558 expressed protein
Length = 318
Score = 28.7 bits (61), Expect = 2.2
Identities = 29/98 (29%), Positives = 50/98 (51%), Gaps = 13/98 (13%)
Frame = +3
Query: 138 KELXRLKKMLGVKDEEKPAGSEVMESEQVIFLD----AGDLKK--SKK-----VLQDIEK 284
K+L ++M + DE+K E+ ++ V+ L+ +LKK SKK L + +K
Sbjct: 178 KKLRSEEEMREIDDEKKREIEELQKTVIVLNLELVKNVEELKKWKSKKKLTEEALSETQK 237
Query: 285 DNEXVEMSSDDENVVVDSEGGKKRVF--NTKTLKDQNG 392
+ +E+ D+ ++ E G K VF N +T+K NG
Sbjct: 238 REKELELKKDE--LLKKVEEGNKTVFALNERTMKPSNG 273
>At4g02510.1 68417.m00343 chloroplast outer membrane protein,
putative similar to chloroplast protein import component
Toc159 [Pisum sativum] GI:8489806, chloroplast outer
envelope protein 86 [Pisum sativum] GI:599958,
GTP-binding protein [Pisum sativum] GI:576509
Length = 1503
Score = 28.3 bits (60), Expect = 3.0
Identities = 13/39 (33%), Positives = 25/39 (64%)
Frame = +3
Query: 210 ESEQVIFLDAGDLKKSKKVLQDIEKDNEXVEMSSDDENV 326
E+E++IF G + +K+ L ++EK + +E SD+ N+
Sbjct: 635 ETEEMIF---GSSEAAKQFLAELEKASSGIEAHSDEANI 670
>At2g16920.1 68415.m01949 ubiquitin-conjugating enzyme family
protein low similarity to ubiquitin-conjugating
BIR-domain enzyme APOLLON [Homo sapiens] GI:8489831,
ubiquitin-conjugating enzyme [Mus musculus] GI:3319990;
contains Pfam profile PF00179: Ubiquitin-conjugating
enzyme
Length = 1102
Score = 28.3 bits (60), Expect = 3.0
Identities = 14/54 (25%), Positives = 24/54 (44%)
Frame = +3
Query: 237 AGDLKKSKKVLQDIEKDNEXVEMSSDDENVVVDSEGGKKRVFNTKTLKDQNGQY 398
AGD + + E D++ + DDE+V + ++ V N K +G Y
Sbjct: 58 AGDSDSDSDISDEEEDDDDDEDNDDDDEDVEEGKKASEENVVNGDGEKKADGNY 111
>At2g03140.1 68415.m00267 CAAX amino terminal protease family protein
very low similarity to SP|Q40863 Late embryogenesis
abundant protein EMB8 from Picea glauca; contains Pfam
profile PF02517 CAAX amino terminal protease family
protein
Length = 1805
Score = 28.3 bits (60), Expect = 3.0
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +3
Query: 153 LKKMLGVKDEEKPAGSEVMESEQVIFLDAGDLKKSKKVLQDIEKDNE 293
L+ M+ DEEK G+EV S++ D +LK +K V ++ +E
Sbjct: 966 LENMISQLDEEKKEGNEV--SDEKNLKDEKNLKDAKNVTNEVVSPSE 1010
>At3g62360.1 68416.m07005 expressed protein
Length = 1227
Score = 27.9 bits (59), Expect = 3.9
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = +2
Query: 206 YGI*ASYISGRGRFKEKQKSITRHRKG 286
YG+ A I G G F E S+ R RKG
Sbjct: 22 YGVSADSIKGCGGFVEASSSLVRSRKG 48
>At1g65010.1 68414.m07368 expressed protein similar to
endosome-associated protein (GI:1016368) [Homo sapiens];
similar to Centromeric protein E (CENP-E protein)
(Swiss-Prot:Q02224) [Homo sapiens]
Length = 1318
Score = 27.5 bits (58), Expect = 5.2
Identities = 20/61 (32%), Positives = 32/61 (52%), Gaps = 2/61 (3%)
Frame = +3
Query: 141 ELXRLKKMLGVKDE-EKPAGSEVMES-EQVIFLDAGDLKKSKKVLQDIEKDNEXVEMSSD 314
EL RLK +LG K+E E G+E++ + I L G+L+K + +++ VE
Sbjct: 207 ELGRLKALLGSKEEKEAIEGNEIVSKLKSEIELLRGELEKVSILESSLKEQEGLVEQLKV 266
Query: 315 D 317
D
Sbjct: 267 D 267
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 27.5 bits (58), Expect = 5.2
Identities = 21/80 (26%), Positives = 33/80 (41%)
Frame = +3
Query: 165 LGVKDEEKPAGSEVMESEQVIFLDAGDLKKSKKVLQDIEKDNEXVEMSSDDENVVVDSEG 344
+G K EKP E+ S + L + L D+E D E E D + E
Sbjct: 39 IGTKLCEKPKAEEIDFSARKKKLKSKKGGSVSFALLDVEDDKEEAEDGDDKNPSIRSDEV 98
Query: 345 GKKRVFNTKTLKDQNGQYPV 404
+ + K KD++G++ V
Sbjct: 99 VGETSMSKKKKKDKSGKHGV 118
>At5g57120.1 68418.m07132 expressed protein weak similarity to
SP|Q14978 Nucleolar phosphoprotein p130 {Homo sapiens}
Length = 330
Score = 27.1 bits (57), Expect = 6.9
Identities = 28/110 (25%), Positives = 58/110 (52%), Gaps = 5/110 (4%)
Frame = +3
Query: 81 IRSRWKRKCRAIK-RERYAVKELXRLKKMLGVKDEEKPAG-SEVMESEQVIFLDAGDLKK 254
++ + K+K +K E VKE + + GVK+++K S+ +E++ + K+
Sbjct: 121 VKEKKKKKETKVKVTEEEKVKETDAVIED-GVKEKKKKKSKSKSVEADDDKEKVSKKRKR 179
Query: 255 SK--KVLQDIEKDNEXVEMSSDDENVVVDSEGGKKR-VFNTKTLKDQNGQ 395
S+ + ++ E D+E + +ENVV + EG ++ V T+T ++ N +
Sbjct: 180 SEPEETKEETEDDDEESKRRKKEENVVENDEGVQETPVKETETKENGNAE 229
>At5g46600.1 68418.m05737 expressed protein contains Pfam profile
PF01027: Uncharacterized protein family UPF0005;
expression supported by MPSS
Length = 539
Score = 27.1 bits (57), Expect = 6.9
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +3
Query: 282 KDNEXVEMSSDDENVVVDSEGGKKRVFNTKTLKDQNGQYPVW 407
KDNE + SDDE+++ + G V ++K+ + Y W
Sbjct: 246 KDNETSDSESDDEDLIYN---GYNTVLDSKSADEALAMYAKW 284
>At3g52860.1 68416.m05825 expressed protein
Length = 156
Score = 27.1 bits (57), Expect = 6.9
Identities = 18/63 (28%), Positives = 30/63 (47%)
Frame = +3
Query: 114 IKRERYAVKELXRLKKMLGVKDEEKPAGSEVMESEQVIFLDAGDLKKSKKVLQDIEKDNE 293
+KRE A LKK + V +EE E+++ +F ++ K V + IEK +
Sbjct: 97 LKREDRAPSRAESLKKDIAVMEEELKTKDELIKKHMRLFQES-----QKLVKEQIEKHRD 151
Query: 294 XVE 302
+E
Sbjct: 152 ELE 154
>At3g14900.1 68416.m01884 expressed protein
Length = 611
Score = 27.1 bits (57), Expect = 6.9
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +3
Query: 273 DIEKDNEXVEMSSDDENVVVDSEG 344
D + D+ VE DD+N+VVD EG
Sbjct: 447 DDDDDDGEVEEVDDDDNMVVDVEG 470
>At4g14200.1 68417.m02191 expressed protein
Length = 784
Score = 26.6 bits (56), Expect = 9.1
Identities = 19/76 (25%), Positives = 40/76 (52%), Gaps = 2/76 (2%)
Frame = +3
Query: 165 LGVKDEEKPAGSEVMESEQVIFLDAGDLKKSKKVLQDIE-KDNEXVEMSS-DDENVVVDS 338
LG+K E GSEV S++++ D + + ++ E E + +S + E++ + +
Sbjct: 355 LGIKPEGLSIGSEVSSSDKLLAGDKTEPQGQTDLVAVKEFPSAENILISKINSEDIGMKA 414
Query: 339 EGGKKRVFNTKTLKDQ 386
E GK + N +T++ +
Sbjct: 415 EEGKSSLGNAQTVESE 430
>At1g52980.1 68414.m05995 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 576
Score = 26.6 bits (56), Expect = 9.1
Identities = 16/40 (40%), Positives = 20/40 (50%)
Frame = +3
Query: 228 FLDAGDLKKSKKVLQDIEKDNEXVEMSSDDENVVVDSEGG 347
F D DLK KK + E D E + +DE+ V SE G
Sbjct: 514 FYDEKDLKDDKKAKESTETDAENGTDAEEDEDAV--SEDG 551
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,910,513
Number of Sequences: 28952
Number of extensions: 168429
Number of successful extensions: 669
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 668
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 858708096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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