Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_H16
         (652 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni...    31   0.11 
SPAC664.06 |rpl703|rpl7|60S ribosomal protein L7|Schizosaccharom...    31   0.14 
SPAC30C2.08 |||conserved fungal protein|Schizosaccharomyces pomb...    30   0.25 
SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom...    28   1.3  
SPAC17H9.19c |cdt2|sev1|WD repeat protein Cdt2|Schizosaccharomyc...    27   1.8  
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos...    27   1.8  
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc...    27   2.3  
SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch...    27   2.3  
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo...    27   2.3  
SPAC23A1.16c |||DUF408 family protein|Schizosaccharomyces pombe|...    27   3.1  
SPBP23A10.15c |qcr1|mas1|mitochondrial processing peptidase comp...    26   4.1  
SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M...    26   5.4  
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb...    25   7.2  
SPBC19F5.04 |||aspartate kinase |Schizosaccharomyces pombe|chr 2...    25   7.2  
SPCC4B3.12 |set9||histone lysine methyltransferase Set9|Schizosa...    25   9.5  

>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
           Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 452

 Score = 31.5 bits (68), Expect = 0.11
 Identities = 17/30 (56%), Positives = 21/30 (70%)
 Frame = +3

Query: 120 TVSDDLTRENSASEISKCIKDLSANNRNLS 209
           ++S  LTRE+S  E  K IKDLS  NR+LS
Sbjct: 243 SISAVLTRESSKYEKGKVIKDLSYLNRDLS 272


>SPAC664.06 |rpl703|rpl7|60S ribosomal protein
           L7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 249

 Score = 31.1 bits (67), Expect = 0.14
 Identities = 14/63 (22%), Positives = 32/63 (50%)
 Frame = +2

Query: 338 IINRKITMSAKSTTPRKRNQRITTRLSQENINKCNKSXXRQRARTFVTWYKQMIDNERQN 517
           ++  ++ +  +    R R +R+   ++++   K N+    +RA TF+  Y+Q    ER+ 
Sbjct: 12  MLEPEVLLKKRKVNERTRKERVEQAIAKKEAQKKNRKETFKRAETFINNYRQ---RERER 68

Query: 518 LAL 526
           + L
Sbjct: 69  IRL 71


>SPAC30C2.08 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 457

 Score = 30.3 bits (65), Expect = 0.25
 Identities = 22/93 (23%), Positives = 43/93 (46%), Gaps = 3/93 (3%)
 Frame = +3

Query: 84  CFTAVEDCKMNSTVSDDLTRENSASEISKCIKDLSANNRNLSLLHK-ELLEINLSV--KT 254
           CF+ +ED K    +SD++       +I   + +L    ++L+L H+  L E +L V   +
Sbjct: 248 CFSFLEDAKTKEGLSDEMKSSPKLQQIYHRLDELLNKLKHLTLTHRWTLRETDLYVYRAS 307

Query: 255 YVQESKKRLQGLRLDLAHEARSSHQVVELLTEK 353
             +    R+ G  LD    A +  +++  L  +
Sbjct: 308 LAEIDSMRIDGQFLDEQGNAPAGQRILLYLLRR 340


>SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 649

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 14/43 (32%), Positives = 25/43 (58%)
 Frame = +2

Query: 338 IINRKITMSAKSTTPRKRNQRITTRLSQENINKCNKSXXRQRA 466
           I+NRK T+  K + P+K+N+  +T + +EN  +   S   + A
Sbjct: 294 ILNRKPTLRKKKSIPKKQNESSST-IQKENTVQQEASSSEEEA 335


>SPAC17H9.19c |cdt2|sev1|WD repeat protein Cdt2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 490

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 19/69 (27%), Positives = 32/69 (46%)
 Frame = +2

Query: 428 INKCNKSXXRQRARTFVTWYKQMIDNERQNLALYLADDAFLEWFGRTIKTRKKVSAFLKH 607
           I K +++  R  + T  TW  Q   +  Q ++   A+ A   W  RT+ T + + A    
Sbjct: 277 IRKAHENSGRDCSITSATWLPQ---STSQVISSCSANSALKLWDLRTVHTVRPLPAATTP 333

Query: 608 DMHCSKHDF 634
           ++  SK DF
Sbjct: 334 ELTTSKRDF 342


>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
           Rad50|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1290

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 24/114 (21%), Positives = 48/114 (42%), Gaps = 4/114 (3%)
 Frame = +3

Query: 147 NSASEISKCIKDLSANNRNLSLLHKELLEINLSVKTYVQESKKRLQGLRLDLAHEARSSH 326
           N  SEI+  ++      + L    + L  + L  + + ++ +K    L +    +   S 
Sbjct: 430 NRVSEINSSLEKQLTTQKELRSRFEILFPVKLQREDFTKDVEK--SDLWIKSLRQEYESK 487

Query: 327 QVVELLTEK*QCQQSQQHRANEIKG*LQDYHR----RTSTNVTNXXKDSELGLL 476
            ++ELL +      S ++R +EI   +  YH+    RT   V    K ++  +L
Sbjct: 488 NLLELLDKHQTALSSVENRLDEISEIVDSYHKYSGVRTKLQVFEENKTNKSAIL 541



 Score = 25.8 bits (54), Expect = 5.4
 Identities = 17/67 (25%), Positives = 35/67 (52%), Gaps = 4/67 (5%)
 Frame = +3

Query: 135  LTREN-SASEISKCIKDLSANNRNLSLLHKELLEINL---SVKTYVQESKKRLQGLRLDL 302
            LT+ N   +E+ +  KD++ ++ +  L  K+LLE++    S   ++ E +   + L  D+
Sbjct: 865  LTKLNFQVNELEQLEKDINKSSEDCDLQKKKLLEVSSKQGSQAPFLNELESEYEKLEADI 924

Query: 303  AHEARSS 323
               A+ S
Sbjct: 925  QEMAQKS 931


>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1233

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
 Frame = +2

Query: 536 DDAFLEWFGRTIKTRKKVSAFLKHDMH-CSKH 628
           DDAF+E+   T+  RK    FL H++   SKH
Sbjct: 691 DDAFVEYKDATVANRKDFIEFLFHNITVSSKH 722


>SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit
            Rev3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1480

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 16/36 (44%), Positives = 22/36 (61%)
 Frame = +3

Query: 108  KMNSTVSDDLTRENSASEISKCIKDLSANNRNLSLL 215
            K+NS  SD L  +N  + ISK +   +A N+ LSLL
Sbjct: 1395 KINSLCSDCL--KNPCATISKAVTQHNAYNKKLSLL 1428


>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7
           domain|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1811

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 15/47 (31%), Positives = 27/47 (57%)
 Frame = +3

Query: 111 MNSTVSDDLTRENSASEISKCIKDLSANNRNLSLLHKELLEINLSVK 251
           + S VSD++   +S  E S   K +  N++NL    ++ +E NLS++
Sbjct: 35  IKSRVSDEIDEHDSIPEQSNTEKSIEINDKNLEA--EKDIESNLSLR 79


>SPAC23A1.16c |||DUF408 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 197

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 14/53 (26%), Positives = 26/53 (49%)
 Frame = +2

Query: 413 LSQENINKCNKSXXRQRARTFVTWYKQMIDNERQNLALYLADDAFLEWFGRTI 571
           +++++I K  K+    + +  +TW K +ID  R  L     D   LEW  + +
Sbjct: 1   MNKKSILKKPKNAQSTQEKQLITWSKSLIDEARSRLQY---DFFALEWIEKLV 50


>SPBP23A10.15c |qcr1|mas1|mitochondrial processing peptidase complex
           beta subunit Qcr1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 457

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 13/32 (40%), Positives = 19/32 (59%)
 Frame = -1

Query: 238 LISKSSLCSNERFLLLAERSFMHLEISEAEFS 143
           +IS +   S+E  + LAE+ F HLE S  + S
Sbjct: 205 IISSAGSISHEELVKLAEKYFGHLEPSAEQLS 236


>SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1052

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 15/27 (55%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
 Frame = +3

Query: 99   EDCK-MNSTVSDDLTRENSASEISKCI 176
            E C  MNST +D++T ENS SEI + I
Sbjct: 961  EQCSCMNSTENDNITTENS-SEIVQVI 986


>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 330

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 21/82 (25%), Positives = 35/82 (42%)
 Frame = +3

Query: 126 SDDLTRENSASEISKCIKDLSANNRNLSLLHKELLEINLSVKTYVQESKKRLQGLRLDLA 305
           S+D  R +S   I   I  + A N   +     LL    +    +  +KK+ +G  +DL 
Sbjct: 9   SNDSVRSSSFKSIKIVI--VGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLN 66

Query: 306 HEARSSHQVVELLTEK*QCQQS 371
           H A  SH+    L +   C+ +
Sbjct: 67  HAAPLSHETRVYLGDYKDCKDA 88


>SPBC19F5.04 |||aspartate kinase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 519

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
 Frame = +3

Query: 105 CKMNSTVSDDLTRENSASEIS-KCIKDLSANNRNLSLLHKELLE 233
           CK+     +       ASEI+  C+ D     + L+++HKELLE
Sbjct: 453 CKLAEAQINIEMISQGASEINISCVIDEKMAVKALNVIHKELLE 496


>SPCC4B3.12 |set9||histone lysine methyltransferase
           Set9|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 441

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
 Frame = -1

Query: 265 SCT*VLTERLISKSSLCSNERFLLLAE-RSFMHLEISEAEFSRVKSSDTVE 116
           SC+   +  +   SSL  + R+++  E RSF+++  S  E S   SSD  E
Sbjct: 257 SCSSKSSSDVSDLSSLPQSNRYVISEEDRSFLNIWDSGGELSDASSSDLDE 307


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,431,987
Number of Sequences: 5004
Number of extensions: 45676
Number of successful extensions: 132
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)