SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_H14
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to autop...   161   3e-40
At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to autop...   155   2e-38
At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to autop...   155   2e-38
At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to autop...   154   6e-38
At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to autop...   153   1e-37
At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to autop...   153   1e-37
At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to autop...   149   2e-36
At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to autop...   146   8e-36
At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to autop...   144   3e-35
At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to autop...   144   3e-35
At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to autop...   122   2e-28
At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to autop...   118   3e-27
At5g26760.2 68418.m03189 expressed protein                             28   4.7  
At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q9...    28   4.7  
At1g49050.1 68414.m05500 aspartyl protease family protein contai...    28   4.7  
At4g14200.1 68417.m02191 expressed protein                             27   8.2  

>At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to
           autophagy 8d [Arabidopsis thaliana] GI:19912157;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 120

 Score =  161 bits (392), Expect = 3e-40
 Identities = 68/114 (59%), Positives = 93/114 (81%)
 Frame = +3

Query: 105 SNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 284
           S++K EH  EKR+AE  +IR KYPDR+PVIVE+A K+ + D+D+KKYLVP+DLTVGQF +
Sbjct: 4   SSFKHEHPLEKRQAEAARIREKYPDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQFVY 63

Query: 285 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 446
           ++RKRI L PE A+F FV N++PPT+A M ++Y+EH DED FLY+++S EN +G
Sbjct: 64  VVRKRIKLSPEKAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFG 117


>At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to
           autophagy 8f [Arabidopsis thaliana] GI:19912161;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 121

 Score =  155 bits (376), Expect = 2e-38
 Identities = 68/114 (59%), Positives = 89/114 (78%)
 Frame = +3

Query: 105 SNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 284
           S++K+EH  EKR+AE  +IR KYPDR+PVIVEKA K+ +  +DKKKYLVP+DLTVGQF +
Sbjct: 4   SSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFVY 63

Query: 285 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 446
           +IRKRI L  E A+F FV+NV+PP  A M S+Y+E  D+D FLY+ +S EN +G
Sbjct: 64  VIRKRIKLSAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFG 117


>At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to
           autophagy 8f [Arabidopsis thaliana] GI:19912161;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 121

 Score =  155 bits (376), Expect = 2e-38
 Identities = 68/114 (59%), Positives = 89/114 (78%)
 Frame = +3

Query: 105 SNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 284
           S++K+EH  EKR+AE  +IR KYPDR+PVIVEKA K+ +  +DKKKYLVP+DLTVGQF +
Sbjct: 4   SSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFVY 63

Query: 285 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 446
           +IRKRI L  E A+F FV+NV+PP  A M S+Y+E  D+D FLY+ +S EN +G
Sbjct: 64  VIRKRIKLSAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFG 117


>At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to
           autophagy 8g [Arabidopsis thaliana] GI:19912163;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3; supporting cDNA
           gi|19912162|dbj|AB073181.1|
          Length = 121

 Score =  154 bits (373), Expect = 6e-38
 Identities = 67/116 (57%), Positives = 92/116 (79%)
 Frame = +3

Query: 102 NSNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFY 281
           N +++++H FEKRKAE  +IR KY DRVPVIVEK+ K+ + ++DKKKYLVP+DLTVGQF 
Sbjct: 3   NVSFRQDHDFEKRKAEALRIREKYSDRVPVIVEKSEKSDIPNIDKKKYLVPADLTVGQFV 62

Query: 282 FLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN 449
           ++IRKRI L  E A+F FV+NV+PPT A M ++Y E+ +ED FLY+ +S EN +G+
Sbjct: 63  YVIRKRIQLSAEKAIFIFVDNVLPPTGAMMSTIYDENKEEDGFLYVTYSGENTFGS 118


>At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to
           autophagy 8e [Arabidopsis thaliana] GI:19912159;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score =  153 bits (371), Expect = 1e-37
 Identities = 70/114 (61%), Positives = 88/114 (77%)
 Frame = +3

Query: 105 SNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 284
           S +K +  FEKRKAE  +IR KYPDR+PVIVEKA K+ + ++DKKKYLVPSDLTVGQF +
Sbjct: 5   SIFKMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVY 64

Query: 285 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 446
           +IRKRI L  E A+F FV+NV+PPT   M S+Y++  DED FLYI +S EN +G
Sbjct: 65  VIRKRIKLSAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118


>At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to
           autophagy 8e [Arabidopsis thaliana] GI:19912159;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score =  153 bits (371), Expect = 1e-37
 Identities = 70/114 (61%), Positives = 88/114 (77%)
 Frame = +3

Query: 105 SNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 284
           S +K +  FEKRKAE  +IR KYPDR+PVIVEKA K+ + ++DKKKYLVPSDLTVGQF +
Sbjct: 5   SIFKMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVY 64

Query: 285 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 446
           +IRKRI L  E A+F FV+NV+PPT   M S+Y++  DED FLYI +S EN +G
Sbjct: 65  VIRKRIKLSAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118


>At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to
           autophagy 8c [Arabidopsis thaliana] GI:19912155;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 119

 Score =  149 bits (361), Expect = 2e-36
 Identities = 64/115 (55%), Positives = 89/115 (77%)
 Frame = +3

Query: 102 NSNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFY 281
           NS++K EH  E+R+ E  +IR KYPDR+PVIVE+A ++ + ++DKKKYLVP+DLTVGQF 
Sbjct: 3   NSSFKLEHPLERRQIESSRIREKYPDRIPVIVERAERSDVPNIDKKKYLVPADLTVGQFV 62

Query: 282 FLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 446
           +++RKRI L  E A+F FV N +PPT+A M ++Y E+ DED FLY+ +S EN +G
Sbjct: 63  YVVRKRIKLSAEKAIFVFVKNTLPPTAAMMSAIYDENKDEDGFLYMTYSGENTFG 117


>At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to
           autophagy 8a [Arabidopsis thaliana] GI:19912151;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score =  146 bits (355), Expect = 8e-36
 Identities = 64/115 (55%), Positives = 88/115 (76%)
 Frame = +3

Query: 105 SNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 284
           S++K  +  E R +E  +IR KYPDR+PVIVEKA ++ + D+DKKKYLVP+DLTVGQF +
Sbjct: 4   SSFKISNPLEARMSESSRIREKYPDRIPVIVEKAGQSDVPDIDKKKYLVPADLTVGQFVY 63

Query: 285 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN 449
           ++RKRI L  E A+F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G+
Sbjct: 64  VVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGS 118


>At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to
           autophagy 8b [Arabidopsis thaliana] GI:19912153;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score =  144 bits (350), Expect = 3e-35
 Identities = 64/117 (54%), Positives = 88/117 (75%)
 Frame = +3

Query: 105 SNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 284
           +++K  +  E R AE  +IR KYP+RVPVIVEKA ++ + D+DKKKYLVP+DLT+GQF +
Sbjct: 4   NSFKLSNPLEMRMAESTRIRAKYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFVY 63

Query: 285 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN*F 455
           ++RKRI L  E A+F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G  F
Sbjct: 64  VVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSF 120


>At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to
           autophagy 8b [Arabidopsis thaliana] GI:19912153;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score =  144 bits (350), Expect = 3e-35
 Identities = 64/117 (54%), Positives = 88/117 (75%)
 Frame = +3

Query: 105 SNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 284
           +++K  +  E R AE  +IR KYP+RVPVIVEKA ++ + D+DKKKYLVP+DLT+GQF +
Sbjct: 4   NSFKLSNPLEMRMAESTRIRAKYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFVY 63

Query: 285 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN*F 455
           ++RKRI L  E A+F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G  F
Sbjct: 64  VVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSF 120


>At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to
           autophagy 8i [Arabidopsis thaliana] GI:19912167;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3; supporting cDNA
           gi|21636957|gb|AF492760.1|
          Length = 115

 Score =  122 bits (294), Expect = 2e-28
 Identities = 52/113 (46%), Positives = 80/113 (70%)
 Frame = +3

Query: 108 NYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFL 287
           ++KE+++ ++R AE  +I  KYP R+PVI EK  K  L  ++KKK+LVP D++VGQF ++
Sbjct: 3   SFKEQYTLDERLAESREIIAKYPTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIYI 62

Query: 288 IRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 446
           +  R+HL P  ALF FVNN +P T+A M S+Y+ + D+D F+Y+ +S E  +G
Sbjct: 63  LSARLHLSPGKALFVFVNNTLPQTAALMDSVYESYKDDDGFVYMCYSSEKTFG 115


>At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to
           autophagy 8h [Arabidopsis thaliana] GI:19912165;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3; supporting cDNA
           gi|19912164|dbj|AB073182.1|
          Length = 119

 Score =  118 bits (284), Expect = 3e-27
 Identities = 53/113 (46%), Positives = 75/113 (66%)
 Frame = +3

Query: 108 NYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFL 287
           ++K++ S ++R  E   I  KYPDR+PVI+EK   A L D++K KYLVP D+TVG F  +
Sbjct: 7   SFKDQFSSDERLKESNNIIAKYPDRIPVIIEKYSNADLPDMEKNKYLVPRDMTVGHFIHM 66

Query: 288 IRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 446
           + KR+ L P  ALF FV+N +P T++ M SLY    +ED FLY+ +S E  +G
Sbjct: 67  LSKRMQLDPSKALFVFVHNTLPQTASRMDSLYNTFKEEDGFLYMCYSTEKTFG 119


>At5g26760.2 68418.m03189 expressed protein
          Length = 735

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 14/33 (42%), Positives = 20/33 (60%)
 Frame = +1

Query: 70  KLQCVIKYQHEIPTTKKNIHLRRERLRAKKSAG 168
           K + V+K Q  +P TKK    RRE+ + KK+ G
Sbjct: 237 KGKTVLKEQTVVPPTKKVSRFRREKEKEKKTFG 269


>At5g06680.1 68418.m00754 tubulin family protein similar to
           SP|Q96CW5 Gamma-tubulin complex component 3 {Homo
           sapiens}
          Length = 838

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 14/37 (37%), Positives = 18/37 (48%), Gaps = 1/37 (2%)
 Frame = +2

Query: 440 LWKLI-PNYSLSNSFTLSDHMVKFNLSLRLKLCIIXW 547
           +WK + PN   SNSF      VK  L   L+ C + W
Sbjct: 622 IWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLW 658


>At1g49050.1 68414.m05500 aspartyl protease family protein contains
           Pfam PF00026: Eukaryotic aspartyl protease; contains
           similarity to nucellin GI:2290203 from [Hordeum vulgare]
          Length = 583

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = +3

Query: 231 DKKKYLVPSDLTVGQFYFLIRKRIHLRPEDAL 326
           D KK+  P  L +G  + +I +++ ++PED L
Sbjct: 485 DVKKFFRPITLQIGSKWLIISRKLLIQPEDYL 516


>At4g14200.1 68417.m02191 expressed protein
          Length = 784

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 13/46 (28%), Positives = 21/46 (45%)
 Frame = +3

Query: 102 NSNYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKARLGDLDKK 239
           N +  EEHS +  +              P +VEKA   R+GD+ ++
Sbjct: 60  NLDLPEEHSLDDEQKPPTPSPTVVEKSAPTVVEKAANERIGDVSEE 105


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,561,657
Number of Sequences: 28952
Number of extensions: 254156
Number of successful extensions: 717
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 693
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 717
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -