BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_H05 (653 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 173 1e-44 SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 170 2e-43 SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 25 7.2 SPAC22E12.09c |krp1|krp|kexin|Schizosaccharomyces pombe|chr 1|||... 25 9.5 >SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 173 bits (422), Expect = 1e-44 Identities = 85/131 (64%), Positives = 98/131 (74%) Frame = +2 Query: 260 LLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAPSA 439 L+++G+ I V +ERDP I W +GAEYV+ STGVFTT + ASAHL+GGAK+VIISAPS Sbjct: 67 LVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAPSK 126 Query: 440 DAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXXX 619 DAPMFVVGVNL ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM Sbjct: 127 DAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTATQK 186 Query: 620 XXDGPSGKLWR 652 DGPS K WR Sbjct: 187 TVDGPSKKDWR 197 Score = 98.3 bits (234), Expect = 8e-22 Identities = 46/63 (73%), Positives = 53/63 (84%), Gaps = 1/63 (1%) Frame = +1 Query: 76 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 252 K+GINGFGRIGR+VLR +I G QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + Sbjct: 5 KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64 Query: 253 GFL 261 G L Sbjct: 65 GKL 67 >SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase Tdh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 336 Score = 170 bits (413), Expect = 2e-43 Identities = 83/131 (63%), Positives = 97/131 (74%) Frame = +2 Query: 260 LLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAPSA 439 L+++GN I V +ERDP I W +GA+YV+ STGVFTT + ASAHL+GGAK+VIISAPS Sbjct: 67 LVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAPSK 126 Query: 440 DAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXXX 619 DAPM+VVGVN ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM Sbjct: 127 DAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTATQK 186 Query: 620 XXDGPSGKLWR 652 DGPS K WR Sbjct: 187 TVDGPSKKDWR 197 Score = 101 bits (241), Expect = 1e-22 Identities = 47/63 (74%), Positives = 54/63 (85%), Gaps = 1/63 (1%) Frame = +1 Query: 76 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 252 K+GINGFGRIGR+VLR A + K QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D Sbjct: 5 KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64 Query: 253 GFL 261 G L Sbjct: 65 GKL 67 >SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosaccharomyces pombe|chr 2|||Manual Length = 502 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/37 (37%), Positives = 19/37 (51%) Frame = +1 Query: 91 GFGRIGRLVLRASIEKGAQVVAINDPFIGLDYMVYLF 201 GF RI +R EKG QVV D I + + +L+ Sbjct: 298 GFVRIESQKIRQHAEKGEQVVFTGDRVIQTNVVPFLY 334 >SPAC22E12.09c |krp1|krp|kexin|Schizosaccharomyces pombe|chr 1|||Manual Length = 709 Score = 25.0 bits (52), Expect = 9.5 Identities = 14/48 (29%), Positives = 23/48 (47%) Frame = +3 Query: 282 LPFSQKGTLRPFHGEKLGLNMLXSLLVSLPLQIKHLLTWREVLKKLLY 425 L + +GT HG L S + +L L I+ L+WR++ +Y Sbjct: 355 LTTNPEGTCTRSHGGTSAAAPLASAVYALALSIRPDLSWRDIQHITVY 402 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,784,373 Number of Sequences: 5004 Number of extensions: 57259 Number of successful extensions: 182 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 176 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 180 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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