Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_H05
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenas... 175 2e-44
At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenas... 173 9e-44
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 169 1e-42
At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenas... 167 4e-42
At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenas... 118 3e-27
At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenas... 116 2e-26
At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenas... 110 9e-25
At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identi... 29 2.7
At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identi... 29 2.7
At2g44030.1 68415.m05474 kelch repeat-containing F-box family pr... 29 2.7
At4g01330.1 68417.m00173 protein kinase family protein contains ... 29 3.6
At3g03910.1 68416.m00405 glutamate dehydrogenase, putative simil... 29 3.6
At3g63330.1 68416.m07125 protein kinase family protein contains ... 27 8.2
At1g21440.1 68414.m02681 mutase family protein similar to carbox... 27 8.2
>At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 422
Score = 175 bits (427), Expect = 2e-44
Identities = 82/132 (62%), Positives = 97/132 (73%)
Frame = +2
Query: 257 SLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAPS 436
+L +NG K+ V S+RDP IPW GA+YVV S+GVFTT KA++HL+GGAKKVIISAPS
Sbjct: 150 TLEINGKKVNVVSKRDPSEIPWADLGADYVVESSGVFTTLSKAASHLKGGAKKVIISAPS 209
Query: 437 ADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXX 616
ADAPMFVVGVN Y P+ ++SNASCTTNCLAPLAKV+H+ F I+EGLM
Sbjct: 210 ADAPMFVVGVNEHTYQPNMDIVSNASCTTNCLAPLAKVVHEEFGILEGLMTTVHATTATQ 269
Query: 617 XXXDGPSGKLWR 652
DGPS K WR
Sbjct: 270 KTVDGPSMKDWR 281
Score = 86.6 bits (205), Expect = 1e-17
Identities = 40/61 (65%), Positives = 47/61 (77%), Gaps = 1/61 (1%)
Frame = +1
Query: 73 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQ 249
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+ KYDSTHG FKGS+ V
Sbjct: 87 TKVGINGFGRIGRLVLRIATSRDDIEVVAVNDPFIDAKYMAYMLKYDSTHGNFKGSINVI 146
Query: 250 D 252
D
Sbjct: 147 D 147
>At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 420
Score = 173 bits (421), Expect = 9e-44
Identities = 82/132 (62%), Positives = 97/132 (73%)
Frame = +2
Query: 257 SLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAPS 436
+L +NG ++ V S+RDP IPW GAEYVV S+GVFTT +AS+HL+GGAKKVIISAPS
Sbjct: 148 TLEINGKQVKVVSKRDPAEIPWADLGAEYVVESSGVFTTVGQASSHLKGGAKKVIISAPS 207
Query: 437 ADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXX 616
ADAPMFVVGVN Y P+ ++SNASCTTNCLAPLAKV+H+ F I+EGLM
Sbjct: 208 ADAPMFVVGVNEKTYLPNMDIVSNASCTTNCLAPLAKVVHEEFGILEGLMTTVHATTATQ 267
Query: 617 XXXDGPSGKLWR 652
DGPS K WR
Sbjct: 268 KTVDGPSMKDWR 279
Score = 87.4 bits (207), Expect = 7e-18
Identities = 39/61 (63%), Positives = 48/61 (78%), Gaps = 1/61 (1%)
Frame = +1
Query: 73 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQ 249
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+FKYDSTHG +KG++ V
Sbjct: 85 TKVGINGFGRIGRLVLRIATFRDDIEVVAVNDPFIDAKYMAYMFKYDSTHGNYKGTINVI 144
Query: 250 D 252
D
Sbjct: 145 D 145
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 169 bits (412), Expect = 1e-42
Identities = 84/139 (60%), Positives = 99/139 (71%), Gaps = 1/139 (0%)
Frame = +2
Query: 239 LRFRMDSLLVNGNK-IAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKK 415
L+ + D L+ G K + VF R+P+ IPWG+AGA++VV STGVFT DKA+AHL+GGAKK
Sbjct: 63 LKVKDDKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVVESTGVFTDKDKAAAHLKGGAKK 122
Query: 416 VIISAPSADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXX 595
V+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+D F IVEGLM
Sbjct: 123 VVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTV 182
Query: 596 XXXXXXXXXXDGPSGKLWR 652
DGPS K WR
Sbjct: 183 HSITATQKTVDGPSMKDWR 201
Score = 78.6 bits (185), Expect = 3e-15
Identities = 34/59 (57%), Positives = 46/59 (77%), Gaps = 1/59 (1%)
Frame = +1
Query: 58 LADNMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFK 231
+AD +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWK 59
>At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenase,
cytosolic (GAPC) / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase identical to
SP|P25858 Glyceraldehyde 3-phosphate dehydrogenase,
cytosolic (EC 1.2.1.12) {Arabidopsis thaliana}
Length = 338
Score = 167 bits (407), Expect = 4e-42
Identities = 83/139 (59%), Positives = 98/139 (70%), Gaps = 1/139 (0%)
Frame = +2
Query: 239 LRFRMDSLLVNGNK-IAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKK 415
L+ + + L+ G K + VF R+P+ IPW +AGA+YVV STGVFT DKA+AHL+GGAKK
Sbjct: 63 LKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGAKK 122
Query: 416 VIISAPSADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXX 595
V+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+D F IVEGLM
Sbjct: 123 VVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTV 182
Query: 596 XXXXXXXXXXDGPSGKLWR 652
DGPS K WR
Sbjct: 183 HSITATQKTVDGPSMKDWR 201
Score = 79.0 bits (186), Expect = 3e-15
Identities = 35/67 (52%), Positives = 51/67 (76%), Gaps = 2/67 (2%)
Frame = +1
Query: 58 LADNMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFK- 231
+AD +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 232 GSVEVQD 252
++++D
Sbjct: 61 NELKIKD 67
>At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenase,
chloroplast, putative / NADP-dependent
glyceraldehydephosphate dehydrogenase, putative similar
to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A,
chloroplast precursor (EC 1.2.1.13) (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 399
Score = 118 bits (284), Expect = 3e-27
Identities = 55/111 (49%), Positives = 72/111 (64%), Gaps = 1/111 (0%)
Frame = +2
Query: 257 SLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAP- 433
+L V+G I + S+R+P +PWG+ G + V+ TGVF D A HL+ GAKKV+I+AP
Sbjct: 129 ALSVDGKIIKIVSDRNPSNLPWGELGIDLVIEGTGVFVDRDGAGKHLQAGAKKVLITAPG 188
Query: 434 SADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLM 586
D P +VVGVN Y +ISNASCTTNCLAP KV+ F I++G M
Sbjct: 189 KGDIPTYVVGVNAELYSHEDTIISNASCTTNCLAPFVKVLDQKFGIIKGTM 239
Score = 42.3 bits (95), Expect = 3e-04
Identities = 27/59 (45%), Positives = 32/59 (54%), Gaps = 3/59 (5%)
Frame = +1
Query: 76 KIGINGFGRIGRLVLR---ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVE 243
K+ INGFGRIGR LR + VV IND G+ +L KYDST G F V+
Sbjct: 66 KVAINGFGRIGRNFLRCWHGRKDSPLDVVVINDTG-GVKQASHLLKYDSTLGIFDADVK 123
>At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenase
A, chloroplast (GAPA) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A
identical to SP|P25856 Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) {Arabidopsis thaliana}
Length = 396
Score = 116 bits (278), Expect = 2e-26
Identities = 55/111 (49%), Positives = 71/111 (63%), Gaps = 1/111 (0%)
Frame = +2
Query: 257 SLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAPS 436
++ V+G I V S R+P +PW + G + V+ TGVF + A H+E GAKKVII+AP
Sbjct: 126 AISVDGKIIQVVSNRNPSLLPWKELGIDIVIEGTGVFVDREGAGKHIEAGAKKVIITAPG 185
Query: 437 -ADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLM 586
D P +VVGVN AY +ISNASCTTNCLAP KV+ F I++G M
Sbjct: 186 KGDIPTYVVGVNADAYSHDEPIISNASCTTNCLAPFVKVLDQKFGIIKGTM 236
Score = 43.2 bits (97), Expect = 2e-04
Identities = 26/59 (44%), Positives = 33/59 (55%), Gaps = 3/59 (5%)
Frame = +1
Query: 76 KIGINGFGRIGRLVLR---ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVE 243
K+ INGFGRIGR LR + ++AIND G+ +L KYDST G F V+
Sbjct: 63 KVAINGFGRIGRNFLRCWHGRKDSPLDIIAINDTG-GVKQASHLLKYDSTLGIFDADVK 120
>At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenase
B, chloroplast (GAPB) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit B
identical to SP|P25857 Glyceraldehyde 3-phosphate
dehydrogenase B, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit B) {Arabidopsis thaliana}
Length = 447
Score = 110 bits (264), Expect = 9e-25
Identities = 55/114 (48%), Positives = 73/114 (64%), Gaps = 3/114 (2%)
Frame = +2
Query: 254 DSLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAP 433
+++ V+G I V S RDP +PW + G + V+ TGVF A H++ GA KVII+AP
Sbjct: 145 ETISVDGKLIKVVSNRDPLKLPWAELGIDIVIEGTGVFVDGPGAGKHIQAGASKVIITAP 204
Query: 434 S--ADAPMFVVGVNLXAYDPSF-KVISNASCTTNCLAPLAKVIHDNFEIVEGLM 586
+ AD P +V+GVN Y +ISNASCTTNCLAP AKV+ + F IV+G M
Sbjct: 205 AKGADIPTYVMGVNEQDYGHDVANIISNASCTTNCLAPFAKVLDEEFGIVKGTM 258
Score = 45.6 bits (103), Expect = 3e-05
Identities = 27/62 (43%), Positives = 35/62 (56%), Gaps = 3/62 (4%)
Frame = +1
Query: 76 KIGINGFGRIGRLVLR---ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV 246
K+ INGFGRIGR LR + +VV +ND G+ +L KYDS G FK V++
Sbjct: 83 KVAINGFGRIGRNFLRCWHGRKDSPLEVVVLNDSG-GVKNASHLLKYDSMLGTFKAEVKI 141
Query: 247 QD 252
D
Sbjct: 142 VD 143
>At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identical
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 29.1 bits (62), Expect = 2.7
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 85 INGFGRIGRLVLRASIEKGAQVVAIND 165
I GFG +G + EKG ++VA++D
Sbjct: 211 IQGFGNVGSWAAKLISEKGGKIVAVSD 237
>At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identical
to glutamate dehydrogenase 2 (GDH 2) [Arabidopsis
thaliana] SWISS-PROT:Q38946
Length = 411
Score = 29.1 bits (62), Expect = 2.7
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +1
Query: 85 INGFGRIGRLVLRASIEKGAQVVAIND 165
I GFG +G + EKG +VVA++D
Sbjct: 211 IQGFGNVGTWAAKLIHEKGGKVVAVSD 237
>At2g44030.1 68415.m05474 kelch repeat-containing F-box family
protein low similarity to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 380
Score = 29.1 bits (62), Expect = 2.7
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +2
Query: 110 VWCSVLLLKRELKWSL*MTLSSVLTI 187
+WC+++LL+REL W + +V T+
Sbjct: 331 IWCALILLERELVWGVIEWSENVFTL 356
>At4g01330.1 68417.m00173 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 329
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = -3
Query: 186 IVKTDERVIYSDHLSSLFNRSTEHQTANAAKS 91
I KT+ RV++SD +SS +R T +TA+ + S
Sbjct: 103 IGKTEHRVVFSDRVSSGESRGTVSETASYSGS 134
>At3g03910.1 68416.m00405 glutamate dehydrogenase, putative similar
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 28.7 bits (61), Expect = 3.6
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 76 KIGINGFGRIGRLVLRASIEKGAQVVAIND 165
+ I GFG +G + +KG ++VA++D
Sbjct: 208 RFAIQGFGNVGSWAAKLISDKGGKIVAVSD 237
>At3g63330.1 68416.m07125 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 376
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 360 VSLPLQIKHLLTWREVLKKLLYQLPVLMPPCLL 458
V+ L +H+ W E K+L Y+L LM C+L
Sbjct: 262 VTRALLDQHIRGWSENFKELAYKLRSLMEMCIL 294
>At1g21440.1 68414.m02681 mutase family protein similar to
carboxyvinyl-carboxyphosphonate phosphorylmutase
GB:O49290 from [Arabidopsis thaliana]; similar to
carboxyphosphonoenolpyruvate mutase (GI:47149)
[Streptomyces hygroscopicus]; contains Prosite PS00161:
Isocitrate lyase signature
Length = 336
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/52 (25%), Positives = 23/52 (44%)
Frame = +2
Query: 326 KAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAPSADAPMFVVGVNLXAY 481
+ A + TG+ D+A+ ++E GA + AP D + +G Y
Sbjct: 191 RTDARALSAKTGLSDAIDRANLYMEAGADASFVEAPRDDDELKEIGRRTKGY 242
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,738,662
Number of Sequences: 28952
Number of extensions: 310385
Number of successful extensions: 863
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 812
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 853
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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