Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_H04
         (610 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g40290.2 68415.m04961 eukaryotic translation initiation facto...   157   5e-39
At2g40290.1 68415.m04960 eukaryotic translation initiation facto...   157   5e-39
At5g05470.1 68418.m00589 eukaryotic translation initiation facto...   153   9e-38
At3g23700.1 68416.m02980 S1 RNA-binding domain-containing protei...    32   0.26 
At3g59670.1 68416.m06657 expressed protein ; expression supporte...    29   3.2  
At1g74830.1 68414.m08670 expressed protein contains Pfam profile...    27   9.7  
At1g53460.1 68414.m06060 expressed protein                             27   9.7  

>At2g40290.2 68415.m04961 eukaryotic translation initiation factor 2
           subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
           putative similar to Swiss-Prot:P05198 eukaryotic
           translation initiation factor 2 subunit 1 (Eukaryotic
           translation initiation factor 2 alpha subunit)
           (eIF-2-alpha) (EIF- 2alpha) (EIF-2A) [Homo sapiens]
          Length = 241

 Score =  157 bits (381), Expect = 5e-39
 Identities = 85/160 (53%), Positives = 103/160 (64%), Gaps = 1/160 (0%)
 Frame = +3

Query: 105 LSCRFYQEKYPEVEDVVMVNVRSIAEMGAYVHLLXYNNIEGMXXXXXXXXXXXXXXNKLI 284
           L CR Y+ KYPEV+  VM+ V++IA+MGAYV LL YNNIEGM              + LI
Sbjct: 8   LECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSSLI 67

Query: 285 RVGKTEPVVVIRVDKEKGYIDLSKRRVSAEDIYKCTERYAKAKAVNSILRHVAXLLHYET 464
           +VG+ EPV+V+RVDKEKGYIDLSKRRVS EDI  C ERY K+K V+SI+RHVA  L    
Sbjct: 68  KVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVAETL---- 123

Query: 465 SEQLEELYKKTAWYFEXKYXKKASAYDFFKQAAVDP-SVL 581
           S  LE+LY    W     Y +   A++ FK    DP SVL
Sbjct: 124 SIDLEDLYVNIGW---PLYRRHGHAFEAFKILVTDPDSVL 160


>At2g40290.1 68415.m04960 eukaryotic translation initiation factor 2
           subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
           putative similar to Swiss-Prot:P05198 eukaryotic
           translation initiation factor 2 subunit 1 (Eukaryotic
           translation initiation factor 2 alpha subunit)
           (eIF-2-alpha) (EIF- 2alpha) (EIF-2A) [Homo sapiens]
          Length = 344

 Score =  157 bits (381), Expect = 5e-39
 Identities = 85/160 (53%), Positives = 103/160 (64%), Gaps = 1/160 (0%)
 Frame = +3

Query: 105 LSCRFYQEKYPEVEDVVMVNVRSIAEMGAYVHLLXYNNIEGMXXXXXXXXXXXXXXNKLI 284
           L CR Y+ KYPEV+  VM+ V++IA+MGAYV LL YNNIEGM              + LI
Sbjct: 8   LECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSSLI 67

Query: 285 RVGKTEPVVVIRVDKEKGYIDLSKRRVSAEDIYKCTERYAKAKAVNSILRHVAXLLHYET 464
           +VG+ EPV+V+RVDKEKGYIDLSKRRVS EDI  C ERY K+K V+SI+RHVA  L    
Sbjct: 68  KVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVAETL---- 123

Query: 465 SEQLEELYKKTAWYFEXKYXKKASAYDFFKQAAVDP-SVL 581
           S  LE+LY    W     Y +   A++ FK    DP SVL
Sbjct: 124 SIDLEDLYVNIGW---PLYRRHGHAFEAFKILVTDPDSVL 160


>At5g05470.1 68418.m00589 eukaryotic translation initiation factor 2
           subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
           putative similar to SWISS-PROT:P20459 eukaryotic
           translation initiation factor 2 alpha subunit (eIF-2-
           alpha) [Saccharomyces cerevisiae]; identical to cDNA
           cohesin GI:6682280
          Length = 344

 Score =  153 bits (371), Expect = 9e-38
 Identities = 81/161 (50%), Positives = 103/161 (63%), Gaps = 1/161 (0%)
 Frame = +3

Query: 105 LSCRFYQEKYPEVEDVVMVNVRSIAEMGAYVHLLXYNNIEGMXXXXXXXXXXXXXXNKLI 284
           L CR Y+ +YP+V+  VM+ V++IA+MGAYV LL YNNIEGM              + LI
Sbjct: 8   LECRMYESRYPDVDMAVMIQVKTIADMGAYVSLLEYNNIEGMILFSELSRRRIRSISSLI 67

Query: 285 RVGKTEPVVVIRVDKEKGYIDLSKRRVSAEDIYKCTERYAKAKAVNSILRHVAXLLHYET 464
           +VG+TEPV+V+RVD+E+GYIDLSKRRVS ED   C ERY K+K V+SI+RHVA  +  + 
Sbjct: 68  KVGRTEPVMVLRVDRERGYIDLSKRRVSDEDKEACEERYNKSKLVHSIMRHVAETVGVD- 126

Query: 465 SEQLEELYKKTAWYFEXKYXKKASAYDFFKQAAVDP-SVLD 584
              LEELY    W     Y K   A++ FK    DP SV D
Sbjct: 127 ---LEELYVNIGW---PLYKKHGHAFEAFKIVVTDPDSVFD 161


>At3g23700.1 68416.m02980 S1 RNA-binding domain-containing protein
           contains Pfam domain, PF00575: S1 RNA binding domain
          Length = 392

 Score = 32.3 bits (70), Expect = 0.26
 Identities = 21/89 (23%), Positives = 41/89 (46%), Gaps = 3/89 (3%)
 Frame = +3

Query: 141 VEDVVMVNVRSIAEMGAYVHLLXYN---NIEGMXXXXXXXXXXXXXXNKLIRVGKTEPVV 311
           V DV    V S+ + GA++HL   +   ++ G+                ++R G    V+
Sbjct: 195 VGDVFNGRVGSVEDYGAFIHLRFDDGLYHLTGLVHVSEVSWDYVQDVRDVLRDGDEVRVI 254

Query: 312 VIRVDKEKGYIDLSKRRVSAEDIYKCTER 398
           V  +DKEK  I LS +++  + + +  ++
Sbjct: 255 VTNIDKEKSRITLSIKQLEDDPLLETLDK 283


>At3g59670.1 68416.m06657 expressed protein ; expression supported
           by MPSS
          Length = 510

 Score = 28.7 bits (61), Expect = 3.2
 Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
 Frame = +3

Query: 516 KYXKKASAYDFFK-QAAVDPSVLDECG 593
           +Y K+   YD  K +A +DPSVL+ CG
Sbjct: 159 EYPKELELYDQEKLEANIDPSVLESCG 185


>At1g74830.1 68414.m08670 expressed protein contains Pfam profile
           PF04576: Protein of unknown function, DUF593
          Length = 542

 Score = 27.1 bits (57), Expect = 9.7
 Identities = 15/55 (27%), Positives = 30/55 (54%)
 Frame = +3

Query: 312 VIRVDKEKGYIDLSKRRVSAEDIYKCTERYAKAKAVNSILRHVAXLLHYETSEQL 476
           V+  D+EKG    SK  +  +++ + TER +  ++   +L+H+A +L     E +
Sbjct: 467 VVSADEEKG--SESKEGI-VKELSEITERLSTLQSNGDLLKHIADVLDVSEGEAI 518


>At1g53460.1 68414.m06060 expressed protein
          Length = 314

 Score = 27.1 bits (57), Expect = 9.7
 Identities = 14/37 (37%), Positives = 23/37 (62%)
 Frame = +3

Query: 276 KLIRVGKTEPVVVIRVDKEKGYIDLSKRRVSAEDIYK 386
           KLI + K+EPV   R  K+KG + +S++     ++YK
Sbjct: 81  KLIELKKSEPV---RKKKQKGEVVISEQNEKRHNLYK 114


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,537,088
Number of Sequences: 28952
Number of extensions: 205519
Number of successful extensions: 533
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 526
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 533
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1216725696
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)