BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_H03 (651 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 29 0.77 SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 28 1.3 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 1.8 SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 2.3 SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 3.1 SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 27 3.1 SPAP7G5.02c |gua2||GMP synthase [glutamine-hydrolyzing] |Schizos... 26 4.1 SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 4.1 SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 26 5.4 SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 5.4 SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 25 7.2 SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosacc... 25 7.2 SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch... 25 7.2 SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 25 7.2 SPBC16E9.05 |erg6||delta-sterol C-methyltransferase |Schizosacch... 25 9.5 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 25 9.5 SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 25 9.5 SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 25 9.5 SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 25 9.5 SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 9.5 >SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces pombe|chr 3|||Manual Length = 238 Score = 28.7 bits (61), Expect = 0.77 Identities = 17/61 (27%), Positives = 32/61 (52%), Gaps = 3/61 (4%) Frame = +3 Query: 321 LGDANGKAKEALEQSRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESHK 491 + +A KA+++LEQ+ + E L K H + E+ + +EKL A ++ + S + Sbjct: 52 INEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEE 111 Query: 492 L 494 L Sbjct: 112 L 112 >SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 272 Score = 27.9 bits (59), Expect = 1.3 Identities = 17/69 (24%), Positives = 36/69 (52%) Frame = +3 Query: 339 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESHKLAXKVSSNV 518 K+++ LE S Q +E E + P+V++ +EK ++ V+ +E K+S N+ Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKEE-----KISRNL 185 Query: 519 XETNEKLAP 545 ++ ++P Sbjct: 186 RSSSRSISP 194 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 27.5 bits (58), Expect = 1.8 Identities = 29/108 (26%), Positives = 44/108 (40%), Gaps = 8/108 (7%) Frame = +3 Query: 324 GDANGKAKEAL----EQSRQNIERTAEELRKAHPDVE---KNATALREKLQAAVQNTVQE 482 G+ N A+ AL E S +N+E K+ + TA ++ + V QE Sbjct: 333 GNVNLSARRALFEKKESSTKNVENPVSHHLKSPVRTSFPPASTTASKQDSPSTVPVDKQE 392 Query: 483 SHKLAXK-VSSNVXETNEKLAPKIXAAYDXFAKNTXAGDQEDPGRPPT 623 + K K VSSN E+ + A FAK + + + P P T Sbjct: 393 TAKPINKQVSSNETSAQEEPRESVAALRARFAKANVSENNDPPTFPKT 440 >SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 27.1 bits (57), Expect = 2.3 Identities = 15/36 (41%), Positives = 22/36 (61%), Gaps = 1/36 (2%) Frame = +3 Query: 342 AKEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 446 A E +++ +IER+A K A + E+ ATALRE Sbjct: 157 ASERAREAQSSIERSASLREKQAREEAERAATALRE 192 >SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 762 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = -2 Query: 392 LGRALDVLPRLFQSLLGLAVRVSERCPGDSW 300 +G + L RLF SL GL ++ PG+ W Sbjct: 410 VGTVIQGLSRLFSSLYGLRFVPADISPGEVW 440 >SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 309 SRAALGDANGKAKEALEQSRQNIERTAEE 395 S A G NG+AKE E+ R ++T E+ Sbjct: 468 SSAKQGKGNGRAKETPEEKRARKKKTKED 496 >SPAP7G5.02c |gua2||GMP synthase [glutamine-hydrolyzing] |Schizosaccharomyces pombe|chr 1|||Manual Length = 539 Score = 26.2 bits (55), Expect = 4.1 Identities = 19/79 (24%), Positives = 31/79 (39%) Frame = -1 Query: 342 PCRSRLRALPWRLLAKALSCCSTDSEPSFQALLKSCASFDLVSELNCCC*SLMELPWCGV 163 PC ++ ALP++ + LS + A F+L + C + E+ W Sbjct: 49 PCTQKIEALPFKPIGVILSGGPYSVYDDIAPHVDP-AVFELGVPVLGICYGMQEIAWLNG 107 Query: 162 RCP*RSRERRVAPSLLGPE 106 RC ER P+ + E Sbjct: 108 RCVEPGIEREYGPATVSME 126 >SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pombe|chr 2|||Manual Length = 175 Score = 26.2 bits (55), Expect = 4.1 Identities = 10/24 (41%), Positives = 18/24 (75%) Frame = +3 Query: 354 LEQSRQNIERTAEELRKAHPDVEK 425 +EQ+R E T E++++A P++EK Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149 >SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 631 Score = 25.8 bits (54), Expect = 5.4 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = -1 Query: 369 ASTVPKPPWPCRSRLRALPWRLLAKALSCCSTDSEPSFQAL 247 AST+ K PWP + L +P + + CS+ +E ++ + Sbjct: 38 ASTLEKEPWPASTALLVMPG---GRDMGYCSSFNETIYRKI 75 >SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizosaccharomyces pombe|chr 2|||Manual Length = 932 Score = 25.8 bits (54), Expect = 5.4 Identities = 19/69 (27%), Positives = 32/69 (46%), Gaps = 1/69 (1%) Frame = +3 Query: 309 SRAALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQE-S 485 +RAA +AN + E E ++ IE R+A ++ E+ + Q +E S Sbjct: 798 ARAAEEEANRELHEQEEAQKRAIEERTRAAREAKEREQREMAEKLERQRRIQQERDEEIS 857 Query: 486 HKLAXKVSS 512 KLA K ++ Sbjct: 858 RKLAEKAAA 866 >SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 25.4 bits (53), Expect = 7.2 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +3 Query: 396 LRKAHPDVEKNATALREKLQAAVQNTVQE 482 L AH D+E T++RE+L++ + +E Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295 >SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosaccharomyces pombe|chr 1|||Manual Length = 188 Score = 25.4 bits (53), Expect = 7.2 Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 2/75 (2%) Frame = +3 Query: 345 KEALEQSRQNIERTAEELRKAHPD--VEKNATALREKLQAAVQNTVQESHKLAXKVSSNV 518 ++ L + R NI ++ + K+ D + N L+ A+ + V+E + ++ Sbjct: 53 QKRLAKLRANIHLESQVIGKSRIDRMLATNVEKLQTVSHASTLHDVEEFYTSHSAKPLDI 112 Query: 519 XETNEKLAPKIXAAY 563 E NE+L+ + +AY Sbjct: 113 SEINERLSEAVQSAY 127 >SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Schizosaccharomyces pombe|chr 3|||Manual Length = 1108 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/32 (40%), Positives = 19/32 (59%) Frame = +3 Query: 333 NGKAKEALEQSRQNIERTAEELRKAHPDVEKN 428 N K KE E++ + EELR+A PD E++ Sbjct: 22 NEKLKEDFEENVSIDVKIHEELRRALPDYEES 53 >SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.4 bits (53), Expect = 7.2 Identities = 10/27 (37%), Positives = 16/27 (59%) Frame = -1 Query: 375 CSASTVPKPPWPCRSRLRALPWRLLAK 295 C +T+PKPP+ + L LP + A+ Sbjct: 290 CPHTTIPKPPYQSDTDLTELPTKSTAQ 316 >SPBC16E9.05 |erg6||delta-sterol C-methyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 25.0 bits (52), Expect = 9.5 Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 2/47 (4%) Frame = -3 Query: 517 TFEDTXFANLWDSCTVFCTAACS--FSRRAVAFFSTSGWALRSSSAV 383 T + T N+WD TVF T+ +R +V F G A + +S V Sbjct: 297 TGDITKCQNIWDVFTVFRTSRLGKLVTRYSVQFLEKIGVAAKGTSKV 343 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 25.0 bits (52), Expect = 9.5 Identities = 16/52 (30%), Positives = 23/52 (44%), Gaps = 5/52 (9%) Frame = +3 Query: 321 LGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 461 L + N + KE +E + RT +E EKN LRE+L+ A Sbjct: 525 LTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576 >SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transporter Bfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1530 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = -3 Query: 448 FSRRAVAFFSTSGWALRSSSAVRSMF 371 FSR V FFS AL+S S + +MF Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595 >SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 633 Score = 25.0 bits (52), Expect = 9.5 Identities = 23/79 (29%), Positives = 35/79 (44%), Gaps = 8/79 (10%) Frame = +3 Query: 339 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESHKL 494 K KE +E Q ++ +ER E LRK D K+ + +AA+ + + E KL Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186 Query: 495 AXKVSSNVXETNEKLAPKI 551 + T +KL KI Sbjct: 187 YKDMEQENASTIQKLTSKI 205 >SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = +3 Query: 327 DANGKAKEALEQSRQNIERTAEE 395 D+ KAK LEQ+R +ERT E Sbjct: 403 DSIKKAKTELEQARIELERTQRE 425 >SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 25.0 bits (52), Expect = 9.5 Identities = 15/68 (22%), Positives = 31/68 (45%) Frame = +3 Query: 339 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESHKLAXKVSSNV 518 KA + LE+ ++ E + EE+ H + T+ + + + +QE +V + Sbjct: 353 KACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEKIIQEK----SRVDKMI 408 Query: 519 XETNEKLA 542 E +KL+ Sbjct: 409 DEYRQKLS 416 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,884,389 Number of Sequences: 5004 Number of extensions: 29831 Number of successful extensions: 141 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 135 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 139 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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