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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_H03
         (651 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce...    29   0.77 
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S...    28   1.3  
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    27   1.8  
SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    27   2.3  
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc...    27   3.1  
SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p...    27   3.1  
SPAP7G5.02c |gua2||GMP synthase [glutamine-hydrolyzing] |Schizos...    26   4.1  
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb...    26   4.1  
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb...    26   5.4  
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos...    26   5.4  
SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa...    25   7.2  
SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosacc...    25   7.2  
SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch...    25   7.2  
SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa...    25   7.2  
SPBC16E9.05 |erg6||delta-sterol C-methyltransferase |Schizosacch...    25   9.5  
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po...    25   9.5  
SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo...    25   9.5  
SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr...    25   9.5  
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch...    25   9.5  
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    25   9.5  

>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 238

 Score = 28.7 bits (61), Expect = 0.77
 Identities = 17/61 (27%), Positives = 32/61 (52%), Gaps = 3/61 (4%)
 Frame = +3

Query: 321 LGDANGKAKEALEQSRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESHK 491
           + +A  KA+++LEQ+    +   E L K H +    E+   + +EKL A ++   + S +
Sbjct: 52  INEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEE 111

Query: 492 L 494
           L
Sbjct: 112 L 112


>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 272

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 17/69 (24%), Positives = 36/69 (52%)
 Frame = +3

Query: 339 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESHKLAXKVSSNV 518
           K+++ LE S Q +E    E +   P+V++     +EK ++ V+   +E      K+S N+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKEE-----KISRNL 185

Query: 519 XETNEKLAP 545
             ++  ++P
Sbjct: 186 RSSSRSISP 194


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 29/108 (26%), Positives = 44/108 (40%), Gaps = 8/108 (7%)
 Frame = +3

Query: 324 GDANGKAKEAL----EQSRQNIERTAEELRKAHPDVE---KNATALREKLQAAVQNTVQE 482
           G+ N  A+ AL    E S +N+E       K+         + TA ++   + V    QE
Sbjct: 333 GNVNLSARRALFEKKESSTKNVENPVSHHLKSPVRTSFPPASTTASKQDSPSTVPVDKQE 392

Query: 483 SHKLAXK-VSSNVXETNEKLAPKIXAAYDXFAKNTXAGDQEDPGRPPT 623
           + K   K VSSN     E+    + A    FAK   + + + P  P T
Sbjct: 393 TAKPINKQVSSNETSAQEEPRESVAALRARFAKANVSENNDPPTFPKT 440


>SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 642

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 15/36 (41%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
 Frame = +3

Query: 342 AKEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 446
           A E   +++ +IER+A    K A  + E+ ATALRE
Sbjct: 157 ASERAREAQSSIERSASLREKQAREEAERAATALRE 192


>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 762

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -2

Query: 392 LGRALDVLPRLFQSLLGLAVRVSERCPGDSW 300
           +G  +  L RLF SL GL    ++  PG+ W
Sbjct: 410 VGTVIQGLSRLFSSLYGLRFVPADISPGEVW 440


>SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 521

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +3

Query: 309 SRAALGDANGKAKEALEQSRQNIERTAEE 395
           S A  G  NG+AKE  E+ R   ++T E+
Sbjct: 468 SSAKQGKGNGRAKETPEEKRARKKKTKED 496


>SPAP7G5.02c |gua2||GMP synthase [glutamine-hydrolyzing]
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 539

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 19/79 (24%), Positives = 31/79 (39%)
 Frame = -1

Query: 342 PCRSRLRALPWRLLAKALSCCSTDSEPSFQALLKSCASFDLVSELNCCC*SLMELPWCGV 163
           PC  ++ ALP++ +   LS             +   A F+L   +   C  + E+ W   
Sbjct: 49  PCTQKIEALPFKPIGVILSGGPYSVYDDIAPHVDP-AVFELGVPVLGICYGMQEIAWLNG 107

Query: 162 RCP*RSRERRVAPSLLGPE 106
           RC     ER   P+ +  E
Sbjct: 108 RCVEPGIEREYGPATVSME 126


>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 175

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 10/24 (41%), Positives = 18/24 (75%)
 Frame = +3

Query: 354 LEQSRQNIERTAEELRKAHPDVEK 425
           +EQ+R   E T E++++A P++EK
Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149


>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 631

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = -1

Query: 369 ASTVPKPPWPCRSRLRALPWRLLAKALSCCSTDSEPSFQAL 247
           AST+ K PWP  + L  +P     + +  CS+ +E  ++ +
Sbjct: 38  ASTLEKEPWPASTALLVMPG---GRDMGYCSSFNETIYRKI 75


>SPBC17D11.05 |tif32||translation initiation factor
            eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 932

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 19/69 (27%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
 Frame = +3

Query: 309  SRAALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQE-S 485
            +RAA  +AN +  E  E  ++ IE      R+A    ++      E+ +   Q   +E S
Sbjct: 798  ARAAEEEANRELHEQEEAQKRAIEERTRAAREAKEREQREMAEKLERQRRIQQERDEEIS 857

Query: 486  HKLAXKVSS 512
             KLA K ++
Sbjct: 858  RKLAEKAAA 866


>SPBC649.05 |cut12|stf1|spindle pole body protein Cut12
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 548

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = +3

Query: 396 LRKAHPDVEKNATALREKLQAAVQNTVQE 482
           L  AH D+E   T++RE+L++ +    +E
Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295


>SPAC23H4.11c |cnl2||centromere localized protein
           Cnl2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 188

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
 Frame = +3

Query: 345 KEALEQSRQNIERTAEELRKAHPD--VEKNATALREKLQAAVQNTVQESHKLAXKVSSNV 518
           ++ L + R NI   ++ + K+  D  +  N   L+    A+  + V+E +        ++
Sbjct: 53  QKRLAKLRANIHLESQVIGKSRIDRMLATNVEKLQTVSHASTLHDVEEFYTSHSAKPLDI 112

Query: 519 XETNEKLAPKIXAAY 563
            E NE+L+  + +AY
Sbjct: 113 SEINERLSEAVQSAY 127


>SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase
           Ubp22|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1108

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 13/32 (40%), Positives = 19/32 (59%)
 Frame = +3

Query: 333 NGKAKEALEQSRQNIERTAEELRKAHPDVEKN 428
           N K KE  E++     +  EELR+A PD E++
Sbjct: 22  NEKLKEDFEENVSIDVKIHEELRRALPDYEES 53


>SPBC27B12.12c |||CorA family magnesium ion transporter
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 803

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 10/27 (37%), Positives = 16/27 (59%)
 Frame = -1

Query: 375 CSASTVPKPPWPCRSRLRALPWRLLAK 295
           C  +T+PKPP+   + L  LP +  A+
Sbjct: 290 CPHTTIPKPPYQSDTDLTELPTKSTAQ 316


>SPBC16E9.05 |erg6||delta-sterol C-methyltransferase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
 Frame = -3

Query: 517 TFEDTXFANLWDSCTVFCTAACS--FSRRAVAFFSTSGWALRSSSAV 383
           T + T   N+WD  TVF T+      +R +V F    G A + +S V
Sbjct: 297 TGDITKCQNIWDVFTVFRTSRLGKLVTRYSVQFLEKIGVAAKGTSKV 343


>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 670

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 16/52 (30%), Positives = 23/52 (44%), Gaps = 5/52 (9%)
 Frame = +3

Query: 321 LGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 461
           L + N + KE +E     +      RT +E        EKN   LRE+L+ A
Sbjct: 525 LTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576


>SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux
           transporter Bfr1|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1530

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 13/26 (50%), Positives = 16/26 (61%)
 Frame = -3

Query: 448 FSRRAVAFFSTSGWALRSSSAVRSMF 371
           FSR  V FFS    AL+S S + +MF
Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595


>SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 633

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 23/79 (29%), Positives = 35/79 (44%), Gaps = 8/79 (10%)
 Frame = +3

Query: 339 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESHKL 494
           K KE +E   Q ++ +ER  E LRK   D  K+      + +AA+     + +  E  KL
Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186

Query: 495 AXKVSSNVXETNEKLAPKI 551
              +      T +KL  KI
Sbjct: 187 YKDMEQENASTIQKLTSKI 205


>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 803

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = +3

Query: 327 DANGKAKEALEQSRQNIERTAEE 395
           D+  KAK  LEQ+R  +ERT  E
Sbjct: 403 DSIKKAKTELEQARIELERTQRE 425


>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 419

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 15/68 (22%), Positives = 31/68 (45%)
 Frame = +3

Query: 339 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESHKLAXKVSSNV 518
           KA + LE+  ++ E + EE+   H    +  T+ +   +   +  +QE      +V   +
Sbjct: 353 KACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEKIIQEK----SRVDKMI 408

Query: 519 XETNEKLA 542
            E  +KL+
Sbjct: 409 DEYRQKLS 416


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,884,389
Number of Sequences: 5004
Number of extensions: 29831
Number of successful extensions: 141
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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