BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_G13 (572 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1442.16c |zta1|SPCC285.01c|NADPH quinone oxidoreductase/ARE-... 44 1e-05 SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 38 0.001 SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 37 0.002 SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 36 0.006 SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 35 0.010 SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 30 0.21 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 27 2.6 SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 25 6.0 >SPCC1442.16c |zta1|SPCC285.01c|NADPH quinone oxidoreductase/ARE-binding protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 329 Score = 44.4 bits (100), Expect = 1e-05 Identities = 25/66 (37%), Positives = 39/66 (59%), Gaps = 1/66 (1%) Frame = +3 Query: 378 FVRXAIDSPPKTPFILGFECAGEIEQVGENV-TNFKVGDQVVALPEYRAWAELVSVPAXY 554 ++R + + P P+I G E AG + VG+ V +FKVGD+VV L + A+A+ +VP Sbjct: 47 YLRTGLYTAP-LPYIPGKEAAGVVAAVGDKVEADFKVGDRVVYLTPFGAYAQYTNVPTTL 105 Query: 555 VYALPE 572 V + E Sbjct: 106 VSKVSE 111 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 37.9 bits (84), Expect = 0.001 Identities = 18/31 (58%), Positives = 21/31 (67%) Frame = +3 Query: 414 PFILGFECAGEIEQVGENVTNFKVGDQVVAL 506 P ILG E AG +E VG VT +VGD V+AL Sbjct: 67 PVILGHEGAGIVESVGPQVTTVQVGDPVIAL 97 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 37.1 bits (82), Expect = 0.002 Identities = 16/38 (42%), Positives = 22/38 (57%) Frame = +3 Query: 393 IDSPPKTPFILGFECAGEIEQVGENVTNFKVGDQVVAL 506 +D P +LG E AG +E +GE V N + GD V+ L Sbjct: 57 VDPEGAFPIVLGHEGAGIVESIGEGVINVRPGDHVILL 94 >SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 360 Score = 35.5 bits (78), Expect = 0.006 Identities = 16/34 (47%), Positives = 21/34 (61%) Frame = +3 Query: 408 KTPFILGFECAGEIEQVGENVTNFKVGDQVVALP 509 K P ILG E AG + +VG+ V++ K GD V P Sbjct: 60 KKPMILGHESAGVVVEVGKGVSSLKPGDPVAVEP 93 >SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 346 Score = 34.7 bits (76), Expect = 0.010 Identities = 18/48 (37%), Positives = 32/48 (66%) Frame = +2 Query: 257 LTGFGGLKTVKILKKPEPTVGEGEVLIRVKACGLNFQDLIVRQGRHRL 400 ++GF LK + + P+ + GEVL+++KA LN++DLI+ +G + L Sbjct: 11 ISGFDQLKPEEY-EVPQK-LNPGEVLVKLKAASLNYRDLIITKGLYPL 56 Score = 33.9 bits (74), Expect = 0.017 Identities = 16/33 (48%), Positives = 22/33 (66%) Frame = +3 Query: 402 PPKTPFILGFECAGEIEQVGENVTNFKVGDQVV 500 P + P + G + AG IE+VGE+V F+ GD VV Sbjct: 57 PLQLPVVPGSDGAGIIEKVGEDVEGFEKGDSVV 89 >SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 423 Score = 30.3 bits (65), Expect = 0.21 Identities = 12/27 (44%), Positives = 18/27 (66%) Frame = +3 Query: 420 ILGFECAGEIEQVGENVTNFKVGDQVV 500 ILG E G + + G+ V N ++GD+VV Sbjct: 94 ILGHESCGIVAEKGDEVNNLEIGDRVV 120 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 26.6 bits (56), Expect = 2.6 Identities = 10/20 (50%), Positives = 16/20 (80%) Frame = +1 Query: 445 KSSKLAKMSPILRWAIKWWL 504 +SS L K+ P+LR+ I++WL Sbjct: 154 RSSHLLKVRPLLRFLIEFWL 173 >SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 897 Score = 25.4 bits (53), Expect = 6.0 Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 2/44 (4%) Frame = -3 Query: 444 HQRIRSPR*KESSVESRWRP*R--TIKSWKFRPQAFTRIRTSPS 319 H ++P S RWR R + K++ FRP TR+ ++PS Sbjct: 73 HPPSKNPTSSSLSTRKRWRQKRLWSFKNFPFRPP--TRLTSTPS 114 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,002,795 Number of Sequences: 5004 Number of extensions: 35770 Number of successful extensions: 101 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 95 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 100 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 244081442 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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