BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_G03 (650 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomy... 101 7e-23 SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomy... 98 8e-22 SPCC4F11.02 |ptc1||protein phosphatase 2C Ptc1|Schizosaccharomyc... 45 1e-05 SPAC4A8.03c |ptc4||protein phosphatase 2C Ptc4|Schizosaccharomyc... 33 0.047 SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 28 1.3 SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 27 2.3 SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase... 27 3.1 SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr ... 25 7.2 SPBC2F12.05c |||sterol binding ankyrin repeat protein|Schizosacc... 25 9.5 SPAC16E8.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 9.5 SPBC776.06c |||spindle pole body interacting protein |Schizosacc... 25 9.5 >SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomyces pombe|chr 1|||Manual Length = 414 Score = 101 bits (243), Expect = 7e-23 Identities = 49/107 (45%), Positives = 67/107 (62%), Gaps = 5/107 (4%) Frame = +3 Query: 96 MGAYLSEPVTEKVSSDEANDKLECGASSMQGWRVNQEDAHNTILDFDVNT-----SLFAV 260 MG LSEPVTEK S + +N+ + G SSMQGWR++ EDAH+ IL + + FAV Sbjct: 1 MGQTLSEPVTEKHSVNGSNEFVLYGLSSMQGWRISMEDAHSAILSMECSAVKDPVDFFAV 60 Query: 261 YDGHGGAEVATYCSQNLPNYIKNTDAYKSGDYTKALEDAFLGFDATI 401 YDGHGG +VA +C NLP ++ ++ GD+ AL+ +FL D I Sbjct: 61 YDGHGGDKVAKWCGSNLPQILEKNPDFQKGDFVNALKSSFLNADKAI 107 >SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 370 Score = 98.3 bits (234), Expect = 8e-22 Identities = 51/105 (48%), Positives = 66/105 (62%), Gaps = 6/105 (5%) Frame = +3 Query: 96 MGAYLSEPVTEKVSSDEANDKLECGASSMQGWRVNQEDAHNTILDF-DVN-----TSLFA 257 MG LSEPV +K SS + L G S MQGWR++ EDAH +L+F D N TS F Sbjct: 1 MGQTLSEPVLDKHSSSGGDRWLHFGVSHMQGWRISMEDAHCALLNFTDSNSSNPPTSFFG 60 Query: 258 VYDGHGGAEVATYCSQNLPNYIKNTDAYKSGDYTKALEDAFLGFD 392 V+DGHGG VA YC Q+LP+ IK+ ++ G+Y +AL+ FL D Sbjct: 61 VFDGHGGDRVAKYCRQHLPDIIKSQPSFWKGNYDEALKSGFLAAD 105 >SPCC4F11.02 |ptc1||protein phosphatase 2C Ptc1|Schizosaccharomyces pombe|chr 3|||Manual Length = 347 Score = 44.8 bits (101), Expect = 1e-05 Identities = 20/45 (44%), Positives = 25/45 (55%), Gaps = 2/45 (4%) Frame = +3 Query: 183 QGWRVNQEDAHNTILDFDVNTS--LFAVYDGHGGAEVATYCSQNL 311 Q WR + ED H + DF N AVYDGH G + + YC +NL Sbjct: 80 QRWRRSMEDTHICLYDFGGNQDDGFVAVYDGHAGIQASDYCQKNL 124 >SPAC4A8.03c |ptc4||protein phosphatase 2C Ptc4|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 32.7 bits (71), Expect = 0.047 Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = +3 Query: 252 FAVYDGHGGAEVATYCSQNLPNYIKNTD 335 + ++DGHGG E + + S NL I+N D Sbjct: 88 YGLFDGHGGTECSEFLSTNLGKIIENQD 115 >SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 27.9 bits (59), Expect = 1.3 Identities = 15/54 (27%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Frame = +3 Query: 108 LSEPVTEKVSSDEANDKLECGASSMQGWR-VNQEDAHNTILDFDVNTSLFAVYD 266 L + + + S ++ AS+ Q V+Q +A++ I D D +TS+F++ D Sbjct: 31 LDQNLLANLESSTSSSTRSSPASNQQNLEIVSQSEAYHKISDLDDSTSIFSISD 84 >SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 27.1 bits (57), Expect = 2.3 Identities = 14/40 (35%), Positives = 24/40 (60%) Frame = +3 Query: 48 LTYKYLFIV*CELHSTMGAYLSEPVTEKVSSDEANDKLEC 167 L YK I+ + +TMGAY++ VT+K++ + D + C Sbjct: 30 LRYKDGVILAADSRTTMGAYIANRVTDKLT--QLTDNIWC 67 >SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 26.6 bits (56), Expect = 3.1 Identities = 16/71 (22%), Positives = 33/71 (46%), Gaps = 4/71 (5%) Frame = +3 Query: 204 EDAHNTILDFDV---NTSLFAVYDGHGGAEVATYCSQNL-PNYIKNTDAYKSGDYTKALE 371 ED H ++D ++ N + ++DGH G + + Q+L P ++ + Y + Sbjct: 98 EDDHVEVIDRNIDEGNWYFWGIFDGHSGWNTSLFLRQHLVPAVVRELQKCTASYYHQNAC 157 Query: 372 DAFLGFDATIA 404 + L D +I+ Sbjct: 158 PSSLALDKSIS 168 >SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 266 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/48 (29%), Positives = 23/48 (47%) Frame = +3 Query: 90 STMGAYLSEPVTEKVSSDEANDKLECGASSMQGWRVNQEDAHNTILDF 233 S + + +S + K SSD A S++ +N+E A N +L F Sbjct: 186 SELSSVMSSEILPKASSDSAGSFEPPVISNISKELINKEYARNVLLQF 233 >SPBC2F12.05c |||sterol binding ankyrin repeat protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1310 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = +3 Query: 246 SLFAVYDGHGGAEVATYCSQNLPNYIKNTDAYKSG 350 SL Y G+ + A Q++ Y+K YKSG Sbjct: 236 SLLRSYSGNRDSSSAPAVPQHMSGYLKKWTNYKSG 270 >SPAC16E8.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 269 Score = 25.0 bits (52), Expect = 9.5 Identities = 14/43 (32%), Positives = 20/43 (46%) Frame = +3 Query: 519 AHLPLXEVLAKYENKLSSLHRNRLGDTATPLSPCLRAKKNVDE 647 A+ + L K KLSSL N+L P+ KK ++E Sbjct: 51 AYQMMYSKLLKSLPKLSSLEENKLTKLKAPIEDMENTKKELEE 93 >SPBC776.06c |||spindle pole body interacting protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 4/38 (10%) Frame = -3 Query: 357 YNLH----FYKHLCFLCSLVDFVNNMWQLQHLHDRHTP 256 +NLH FY+ L SL+ +NN W +L+ ++P Sbjct: 146 FNLHNFQSFYQKLSLDSSLILALNNFWSFFNLYLTNSP 183 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,800,438 Number of Sequences: 5004 Number of extensions: 59322 Number of successful extensions: 153 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 150 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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