Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_F24
(651 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|... 57 3e-09
SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|ch... 30 0.25
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma... 30 0.25
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo... 29 0.58
SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 28 1.0
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 27 2.3
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 27 3.1
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom... 26 5.4
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr... 25 7.2
SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces po... 25 7.2
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 7.2
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 25 9.5
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual 25 9.5
SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|... 25 9.5
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 25 9.5
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 25 9.5
>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 137
Score = 56.8 bits (131), Expect = 3e-09
Identities = 25/57 (43%), Positives = 36/57 (63%)
Frame = +1
Query: 322 KQKLFLMSWCPDTAKVXKXMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVEEKL 492
+ K+ +SW PD A + M+YSSS D L+++ G+ IQATD SE + E V EK+
Sbjct: 78 RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQATDFSEVAYETVLEKV 134
Score = 52.4 bits (120), Expect = 5e-08
Identities = 27/71 (38%), Positives = 40/71 (56%)
Frame = +3
Query: 72 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 251
SGV VS C ++E+K K RYVVF + D K V + +++ FL DL +
Sbjct: 4 SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPE---K 60
Query: 252 ECRYGLFDFEY 284
+CRY ++DFE+
Sbjct: 61 DCRYAIYDFEF 71
>SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 30.3 bits (65), Expect = 0.25
Identities = 16/53 (30%), Positives = 24/53 (45%), Gaps = 2/53 (3%)
Frame = -1
Query: 411 FQSVERARVQHXLXNLGGVRAPRHQEELLFLASLRRXPGT--GACIQSQTGHI 259
+Q + RA + H + ++ Q E +F+ LRR PGT G C I
Sbjct: 113 YQVLTRACITHNIRHICTAHHANDQAETIFMRLLRRKPGTWGGLCAMKPVSQI 165
>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 328
Score = 30.3 bits (65), Expect = 0.25
Identities = 14/27 (51%), Positives = 19/27 (70%)
Frame = +1
Query: 316 SKKQKLFLMSWCPDTAKVXKXMLYSSS 396
SKK L L+S+ P+ A V + MLY+SS
Sbjct: 76 SKKNLLQLISYVPENANVRRKMLYASS 102
Score = 25.0 bits (52), Expect = 9.5
Identities = 15/56 (26%), Positives = 30/56 (53%), Gaps = 5/56 (8%)
Frame = +1
Query: 328 KLFLMSWCPDTAKVXKXMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 480
K+ + CP A V M+YSSS D++K L + + I++ D ++ +++ +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299
>SPAC17H9.11 |||cofilin/tropomyosin family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 141
Score = 29.1 bits (62), Expect = 0.58
Identities = 17/63 (26%), Positives = 30/63 (47%)
Frame = +1
Query: 304 TSEASKKQKLFLMSWCPDTAKVXKXMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 483
T++ LF++ W P M+Y+S+ + + V K +A D + + EAV+
Sbjct: 79 TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137
Query: 484 EKL 492
E L
Sbjct: 138 EFL 140
>SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 314
Score = 28.3 bits (60), Expect = 1.0
Identities = 24/91 (26%), Positives = 34/91 (37%)
Frame = +1
Query: 286 RTSARXTSEASKKQKLFLMSWCPDTAKVXKXMLYSSSFDALKKSLVGVQKYIQATDLSEA 465
RT T A+K + P T K + ++ A KK + G +KY + S
Sbjct: 124 RTQFAFTGRATKSNAGVMDVQSPSTMSTSKNNVRNAERPASKKPVFGSKKYFDVINDSNV 183
Query: 466 SQEAVEEKLRATDRQ*TAFTHELATKPNPLS 558
+ E K R DR + FT LS
Sbjct: 184 ENKE-ETKDRNLDRALSKFTQSREKNEQNLS 213
>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 214
Score = 27.1 bits (57), Expect = 2.3
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = +1
Query: 394 SFDALKKSLVGVQKYIQATDLSEASQ 471
S +AL++ L G + Y+Q+TD++ S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213
>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 830
Score = 26.6 bits (56), Expect = 3.1
Identities = 13/42 (30%), Positives = 25/42 (59%)
Frame = -1
Query: 435 LLNSDKGLFQSVERARVQHXLXNLGGVRAPRHQEELLFLASL 310
++NS++ ++ + +R +Q + R PRH+E +L LA L
Sbjct: 367 VINSERTVYNNYQRITLQESPGTVPSGRLPRHREVIL-LADL 407
>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 447
Score = 25.8 bits (54), Expect = 5.4
Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 3/60 (5%)
Frame = +3
Query: 456 LGSVSGGRRREAPRHRSPINSIYTRARDE---TEPALRHSCPDDTRPRHH*PLSIITKEN 626
LG G ++ E+ R + P S + EP +RHS RP+ H S +T+++
Sbjct: 20 LGEEDGRQKGESSRKKRPPLSRKNPSNVSFWSNEPIIRHSSVKTDRPQFHRADSTVTEQS 79
>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 319
Score = 25.4 bits (53), Expect = 7.2
Identities = 14/61 (22%), Positives = 31/61 (50%)
Frame = +3
Query: 99 KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 278
K + + ++ + K ++ +D K+ D + + + EY F ++L+ TG+ Y DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166
Query: 279 E 281
+
Sbjct: 167 Q 167
>SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 432
Score = 25.4 bits (53), Expect = 7.2
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = -2
Query: 350 HHDIRKSFCFLLASDVXLALVRVFKVKQAISAFPGTALLQILEELFVF 207
HH S C L + L + + +KQ + + G+ +L++L + +F
Sbjct: 192 HHIRIISKCSLKLKSMVLVSMIIPSIKQTLDFYSGSQILRLLNDFSMF 239
>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 357
Score = 25.4 bits (53), Expect = 7.2
Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 2/46 (4%)
Frame = -3
Query: 298 WHWCVYSKSNRPY--LHSPVPPFCRSSRNCSYSALRSPTVSTSICF 167
W++ Y+ S R Y VPP+CR +R+ + CF
Sbjct: 122 WNFVDYALSLRKYGWTDDVVPPYCRKVLTYKSLGIRTAVYVGAFCF 167
>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 339
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/52 (25%), Positives = 24/52 (46%)
Frame = +1
Query: 199 ATPNTNSSSRICRRAVPGNADMACLTLNTRTSARXTSEASKKQKLFLMSWCP 354
A + +++ RI PGN + TL + + + +Q F ++WCP
Sbjct: 129 AAVSADATLRIYEAMEPGN--LTYWTLMNEIALMPSPPSRNEQPAFCVNWCP 178
>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 366
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/22 (45%), Positives = 11/22 (50%)
Frame = +3
Query: 81 TVSDACKTTYEEIKKDKKHRYV 146
TV D C + IK KH YV
Sbjct: 316 TVEDFCNNIHSSIKSQFKHAYV 337
>SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 584
Score = 25.0 bits (52), Expect = 9.5
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +3
Query: 78 VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDV 182
VT D KT + +K KH+ V YIR K +D+
Sbjct: 113 VTNDDGTKTKVDSDEKKHKHKNVRDYIR-FKHVDI 146
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 25.0 bits (52), Expect = 9.5
Identities = 14/39 (35%), Positives = 17/39 (43%)
Frame = +3
Query: 489 APRHRSPINSIYTRARDETEPALRHSCPDDTRPRHH*PL 605
APR R P ++ T A E P + P TR PL
Sbjct: 24 APRKREPARTVSTPAFMEPAPVSKKPLPPPTRRLPRKPL 62
>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1062
Score = 25.0 bits (52), Expect = 9.5
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 504 SPINSIYTRARDETEPALRHSCPDDTRPRHH*PLSIITKEN 626
SP+++ D ++PAL S P+H LS KEN
Sbjct: 391 SPLSTPLKAQFDPSKPALNRSIDHQKTPQHKRYLSTEFKEN 431
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,329,423
Number of Sequences: 5004
Number of extensions: 43414
Number of successful extensions: 146
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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