BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_F04 (654 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 46 5e-06 SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 43 3e-05 SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 43 4e-05 SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces po... 43 4e-05 SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 40 4e-04 SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb... 34 0.016 SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 29 0.77 SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 25 7.2 >SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 259 Score = 46.0 bits (104), Expect = 5e-06 Identities = 38/148 (25%), Positives = 63/148 (42%), Gaps = 3/148 (2%) Frame = +3 Query: 135 KLAGRTLFITGASRXXXXXXXXXXXXXXX-NVVIAAKTAEPHPKLPGTIYTAAEEIEA-L 308 +L G+T+ ITGAS +++AA+ T+ A+E+E+ Sbjct: 3 RLDGKTILITGASSGIGKSTAFEIAKVAKVKLILAARRFS-------TVEEIAKELESKY 55 Query: 309 GGKALPCIVDVRDXKQVQKAVDEAVKKFNGIDILVNNAS-AISLTGTAQTDMKRYDLMHN 485 LP +DV D K + ++ K+F ID+L+NNA A+ ++ M Sbjct: 56 EVSVLPLKLDVSDLKSIPGVIESLPKEFADIDVLINNAGLALGTDKVIDLNIDDAVTMIT 115 Query: 486 INTRGTFLASXICLPVLKNSNHAHILNL 569 N G + LP+ + N ILN+ Sbjct: 116 TNVLGMMAMTRAVLPIFYSKNKGDILNV 143 >SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 43.2 bits (97), Expect = 3e-05 Identities = 28/109 (25%), Positives = 52/109 (47%), Gaps = 1/109 (0%) Frame = +3 Query: 318 ALPCIVDVRDXKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQTDMKRYDLMHNINTR 497 AL DV Q++ A+++ ++ ID+L+NNA T Q + +D ++ R Sbjct: 47 ALALQADVSKADQIETAIEKVIQTLGPIDVLINNAGLADDTPFEQLSHESWDHDVSLVLR 106 Query: 498 GTFLASXICLP-VLKNSNHAHILNLSPPLNMNPYWFSIHVAYTMAKYGM 641 G +L +P + K I+N+ ++N + + AY+ AK G+ Sbjct: 107 GNYLTQRYVIPHMAKQGKGGSIVNIG---SVNGHIYLGSPAYSAAKAGL 152 >SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 236 Score = 42.7 bits (96), Expect = 4e-05 Identities = 20/80 (25%), Positives = 41/80 (51%) Frame = +3 Query: 333 VDVRDXKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQTDMKRYDLMHNINTRGTFLA 512 +DV + ++K+V++A+ F ID+L+NNA + T ++ + N N G Sbjct: 21 LDVNNFSSIKKSVEKAISHFGRIDVLLNNAGYSVYSPLESTTEEQIHNIFNTNVFGALEV 80 Query: 513 SXICLPVLKNSNHAHILNLS 572 P+ ++ ++ I+N+S Sbjct: 81 IKAITPIFRSQHNGMIINVS 100 >SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 236 Score = 42.7 bits (96), Expect = 4e-05 Identities = 20/80 (25%), Positives = 41/80 (51%) Frame = +3 Query: 333 VDVRDXKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQTDMKRYDLMHNINTRGTFLA 512 +DV + ++K+V++A+ F ID+L+NNA + T ++ + N N G Sbjct: 21 LDVNNFSSIKKSVEKAISHFGRIDVLLNNAGYSVYSPLESTTEEQIHNIFNTNVFGALEV 80 Query: 513 SXICLPVLKNSNHAHILNLS 572 P+ ++ ++ I+N+S Sbjct: 81 IKAITPIFRSQHNGMIINVS 100 >SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 292 Score = 39.5 bits (88), Expect = 4e-04 Identities = 33/126 (26%), Positives = 57/126 (45%), Gaps = 3/126 (2%) Frame = +3 Query: 285 AAEEIEALGGKALPCIVDVRDXKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQT-DM 461 A + I K L +DV D K V+ A +A ++F +DI++NNA L G ++ ++ Sbjct: 38 APDTITIEHSKLLKLKLDVTDVKSVETAFKDAKRRFGNVDIVINNA-GYGLVGEFESYNI 96 Query: 462 KRYDLMHNINTRGTFLASXICLPVLKNSNH-AHILNLSPPLNMNPY-WFSIHVAYTMAKY 635 + N+N G + L +++ S IL +S P S++ A A Sbjct: 97 EEMHRQMNVNFWGVAYITKEALNLMRESGKGGRILQISSVAGYYPSPCLSMYNASKFAVE 156 Query: 636 GMSMCV 653 G+S + Sbjct: 157 GLSQTI 162 >SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 339 Score = 34.3 bits (75), Expect = 0.016 Identities = 31/136 (22%), Positives = 54/136 (39%) Frame = +3 Query: 138 LAGRTLFITGASRXXXXXXXXXXXXXXXNVVIAAKTAEPHPKLPGTIYTAAEEIEALGGK 317 L G+ +TG+S V +A + E + K+ I+ E K Sbjct: 40 LTGKVALVTGSSGGIGYVTALELARKGAKVYLAGRNEEKYQKVMKQIHD-----EVRHSK 94 Query: 318 ALPCIVDVRDXKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQTDMKRYDLMHNINTR 497 +D+ D + V +A + + K + ILVNNA ++ D Y+L N Sbjct: 95 IRFLRLDLLDFESVYQAAESFIAKEEKLHILVNNAGIMNPPFELTKD--GYELQIQTNYL 152 Query: 498 GTFLASXICLPVLKNS 545 +L + + LP L+ + Sbjct: 153 SHYLFTELLLPTLRRT 168 >SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 286 Score = 28.7 bits (61), Expect = 0.77 Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 1/61 (1%) Frame = +3 Query: 369 VDEAVKKFNGIDILVNNASAISLTGTAQ-TDMKRYDLMHNINTRGTFLASXICLPVLKNS 545 VD A+KK ID+LVNN + + + + D +++DL N F + + +K+ Sbjct: 112 VDFALKKLGWIDVLVNNIAYQQVAQSIEDIDDEQWDLTFKTNIFSFFWVTKAAISHMKSG 171 Query: 546 N 548 + Sbjct: 172 S 172 >SPBC36.10 |||mitochondrial intermembrane space protein sorting protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 25.4 bits (53), Expect = 7.2 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = +3 Query: 243 TAEPHPKLPGTIYTAAEEIEAL 308 T PHP+L T++ IEAL Sbjct: 110 TYSPHPELEATVFQQEARIEAL 131 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,366,259 Number of Sequences: 5004 Number of extensions: 42970 Number of successful extensions: 87 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 85 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 86 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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