SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_D05
         (647 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy...   134   1e-32
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar...   123   3e-29
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar...    89   7e-19
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo...    62   7e-11
SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||...    43   4e-05
SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|...    42   8e-05
SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||...    34   0.015
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit...    33   0.027
SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|ch...    32   0.062
SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||...    32   0.062
SPAC23C4.08 |rho3||Rho family GTPase Rho3|Schizosaccharomyces po...    30   0.25 
SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||...    30   0.33 
SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces po...    30   0.33 
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma...    29   0.76 
SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch...    29   0.76 
SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr...    28   1.0  
SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces ...    28   1.3  
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ...    27   1.8  
SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||...    27   2.3  
SPAC23H4.07c |srp102||signal recognition particle receptor beta ...    26   5.4  
SPAC4F10.10c |||mannosyltransferase complex subunit, Anp family ...    25   9.4  
SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Sc...    25   9.4  
SPAC2G11.13 |atg22||autophagy associated protein Atg22 |Schizosa...    25   9.4  

>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 180

 Score =  134 bits (323), Expect = 1e-32
 Identities = 60/89 (67%), Positives = 71/89 (79%)
 Frame = +1

Query: 220 FRGLLGAREMRILILGLDGAGKTTILYKLQVGEVVTTIPTIGFNVXQVTYKNLKFQVWDL 399
           F+ L G REMRIL++GLD AGKTTILYKL++GE+VTTIPTIGFNV  V Y+N+ F VWD+
Sbjct: 9   FQSLFGKREMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDV 68

Query: 400 GGQTSIRPYWRCYYGNTDAIIYVVXSXDR 486
           GGQ  IRP WR Y+ NT  II+VV S DR
Sbjct: 69  GGQDKIRPLWRHYFQNTQGIIFVVDSNDR 97



 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 22/51 (43%), Positives = 31/51 (60%)
 Frame = +2

Query: 491 RIGISKDELVHMLXEXXLXNAILVVLANKQDMAGCLTVAXVHQALGLDALR 643
           RI  + +EL  ML E  L +A+L+V ANKQD+   +  A +   LGL +LR
Sbjct: 99  RISEAHEELQRMLNEDELRDALLLVFANKQDLPNAMNAAEITDKLGLHSLR 149


>SPBC1539.08 |||ADP-ribosylation factor, Arf
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score =  123 bits (296), Expect = 3e-29
 Identities = 59/98 (60%), Positives = 70/98 (71%)
 Frame = +1

Query: 205 GLFSYFRGLLGAREMRILILGLDGAGKTTILYKLQVGEVVTTIPTIGFNVXQVTYKNLKF 384
           G    F  L   +EMRIL+LGLD AGKTTILYKL++ + V TIPT+GFNV  VTYKN+KF
Sbjct: 8   GFSKPFSRLFSNKEMRILMLGLDAAGKTTILYKLKLNQSVVTIPTVGFNVETVTYKNIKF 67

Query: 385 QVWDLGGQTSIRPYWRCYYGNTDAIIYVVXSXDRASNR 498
            VWD+GGQ  IRP WR Y+  T  +I+VV S D  SNR
Sbjct: 68  NVWDVGGQDKIRPLWRHYFTGTKGLIFVVDSAD--SNR 103



 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 20/53 (37%), Positives = 34/53 (64%)
 Frame = +2

Query: 488 HRIGISKDELVHMLXEXXLXNAILVVLANKQDMAGCLTVAXVHQALGLDALRD 646
           +RI  ++ EL  ++ +  + + +L+VLANKQD+ G L+ A +   L LD L+D
Sbjct: 102 NRISEARQELHRIISDREMRDCLLLVLANKQDLPGALSPAQITDVLQLDKLKD 154


>SPAC22F3.05c |alp41||ADP-ribosylation factor
           Alp41|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 186

 Score = 88.6 bits (210), Expect = 7e-19
 Identities = 40/84 (47%), Positives = 58/84 (69%)
 Frame = +1

Query: 232 LGAREMRILILGLDGAGKTTILYKLQVGEVVTTIPTIGFNVXQVTYKNLKFQVWDLGGQT 411
           L  RE+R+L+LGLD AGKTTIL  L   +V    PT GF +  +  + L+F +WD+GGQ 
Sbjct: 12  LKEREVRVLLLGLDNAGKTTILKCLLNEDVNEVSPTFGFQIRTLEVEGLRFTIWDIGGQK 71

Query: 412 SIRPYWRCYYGNTDAIIYVVXSXD 483
           ++R +W+ Y+ +T+AII+VV S D
Sbjct: 72  TLRNFWKNYFESTEAIIWVVDSLD 95


>SPBC31F10.06c |sar1||ADP-ribosylation factor
           Sar1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 190

 Score = 62.1 bits (144), Expect = 7e-11
 Identities = 28/81 (34%), Positives = 45/81 (55%)
 Frame = +1

Query: 241 REMRILILGLDGAGKTTILYKLQVGEVVTTIPTIGFNVXQVTYKNLKFQVWDLGGQTSIR 420
           +  ++L LGLD AGKTT+L+ L+   +    PT+     ++   N++F  +DLGG    R
Sbjct: 19  KHAKMLFLGLDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELAIGNVRFTTFDLGGHQQAR 78

Query: 421 PYWRCYYGNTDAIIYVVXSXD 483
             WR Y+   + I+Y+V   D
Sbjct: 79  RLWRDYFPEVNGIVYLVDCCD 99


>SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 203

 Score = 42.7 bits (96), Expect = 4e-05
 Identities = 27/90 (30%), Positives = 41/90 (45%), Gaps = 5/90 (5%)
 Frame = +1

Query: 250 RILILGLDGAGKTTILYKLQVGEVVTT-IPTIGFNVXQVTY----KNLKFQVWDLGGQTS 414
           ++L++G  G GK+ +L +        + I TIG +    T+    K +K Q+WD  GQ  
Sbjct: 10  KLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTFELEGKTVKLQIWDTAGQER 69

Query: 415 IRPYWRCYYGNTDAIIYVVXSXDRASNRNI 504
            R     YY     II V    D+ S  N+
Sbjct: 70  FRTITSSYYRGAHGIIIVYDVTDQDSFNNV 99


>SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 200

 Score = 41.9 bits (94), Expect = 8e-05
 Identities = 24/91 (26%), Positives = 42/91 (46%), Gaps = 5/91 (5%)
 Frame = +1

Query: 247 MRILILGLDGAGKTTILYKLQVGEVVTT-IPTIGFN----VXQVTYKNLKFQVWDLGGQT 411
           +++L++G  G GK+ +L +        + I TIG +      ++  K +K Q+WD  GQ 
Sbjct: 10  IKLLLIGDSGVGKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQE 69

Query: 412 SIRPYWRCYYGNTDAIIYVVXSXDRASNRNI 504
             R     YY     I+ +    D+ S  N+
Sbjct: 70  RFRTITTAYYRGAMGILLLYDVTDKKSFDNV 100


>SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 214

 Score = 34.3 bits (75), Expect = 0.015
 Identities = 24/92 (26%), Positives = 40/92 (43%), Gaps = 5/92 (5%)
 Frame = +1

Query: 250 RILILGLDGAGKTTILYKLQVGEV-VTTIPTIG--FNVXQVTYKN--LKFQVWDLGGQTS 414
           + +++G  G GK+ +L +    E  + +  TIG  F    +   N  +K Q+WD  GQ  
Sbjct: 12  KTVLIGDSGVGKSNLLMRFTRNEFNIESKSTIGVEFATRNIVLDNKKIKAQIWDTAGQER 71

Query: 415 IRPYWRCYYGNTDAIIYVVXSXDRASNRNI*R 510
            R     YY      + V     ++S  N+ R
Sbjct: 72  YRAITSAYYRGAVGALIVYDITKQSSFDNVGR 103


>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
           Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 354

 Score = 33.5 bits (73), Expect = 0.027
 Identities = 15/49 (30%), Positives = 28/49 (57%)
 Frame = +1

Query: 337 TIGFNVXQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVXSXD 483
           T+G +    T  +L+ +++D+GGQ + R  W   + N ++II+ V   D
Sbjct: 179 TLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLND 227


>SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 216

 Score = 32.3 bits (70), Expect = 0.062
 Identities = 23/90 (25%), Positives = 38/90 (42%), Gaps = 5/90 (5%)
 Frame = +1

Query: 250 RILILGLDGAGKTTILYKLQVGEVVTT-IPTIGFNV----XQVTYKNLKFQVWDLGGQTS 414
           +++++G  G GKTT + +   GE     I T+G  V        +  + F VWD  GQ  
Sbjct: 11  KLVLVGDGGTGKTTFVKRHLTGEFEKKYIATLGVEVHPLHFHTNFGEICFNVWDTAGQEK 70

Query: 415 IRPYWRCYYGNTDAIIYVVXSXDRASNRNI 504
           +      YY      I +     R + +N+
Sbjct: 71  LGGLRDGYYIQGQCGIIMFDVTSRITYKNV 100


>SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 234

 Score = 32.3 bits (70), Expect = 0.062
 Identities = 28/93 (30%), Positives = 43/93 (46%), Gaps = 14/93 (15%)
 Frame = +1

Query: 247 MRILILGLDGAGKTTILYKL-------QVGEVV-----TTIPTIGFNVXQVTYKNLKFQV 390
           ++I++ G  G GK+ +L +        QV   V     + I ++G    Q   K +K Q+
Sbjct: 10  VKIVLAGPSGTGKSCLLQRFVKNQWDDQVSHTVGIDFASRIISVGMGNQQ---KRIKLQI 66

Query: 391 WDLGGQTSIRPYWRCYY-GNTDAI-IYVVXSXD 483
           WD  GQ   R   R YY G   A+ +Y V + D
Sbjct: 67  WDTAGQEKFRSVARNYYRGAAGAVLVYDVTNKD 99


>SPAC23C4.08 |rho3||Rho family GTPase Rho3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 205

 Score = 30.3 bits (65), Expect = 0.25
 Identities = 26/88 (29%), Positives = 37/88 (42%), Gaps = 6/88 (6%)
 Frame = +1

Query: 250 RILILGLDGAGKTTILYKLQVGEVVTTI-PTIGFNVXQ---VTYKNLKFQVWDLGGQTSI 417
           +I+ILG   AGKT++L     G       PTI  N      V   +++  +WD  GQ   
Sbjct: 15  KIVILGDGAAGKTSLLNVFTKGYFPQVYEPTIFENYIHDIFVDGNSIELSLWDTAGQEEY 74

Query: 418 RPYWRCYYGNTDAII--YVVXSXDRASN 495
                  Y +T  I+  + V S D   N
Sbjct: 75  DQLRSLSYSDTHVIMICFAVDSRDSLEN 102


>SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 205

 Score = 29.9 bits (64), Expect = 0.33
 Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 5/79 (6%)
 Frame = +1

Query: 247 MRILILGLDGAGKTTILYKLQVGEVVTTI-PTIG--FNVXQVTY--KNLKFQVWDLGGQT 411
           ++++ILG  G GKT+I+ +    +       TIG  F   +V    K +  Q+WD  GQ 
Sbjct: 9   LKVIILGESGVGKTSIMNQYVNRKFSKDYKATIGADFLTKEVLVDDKVVTLQLWDTAGQE 68

Query: 412 SIRPYWRCYYGNTDAIIYV 468
             +     +Y   D  + V
Sbjct: 69  RFQSLGVAFYRGADCCVLV 87


>SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1057

 Score = 29.9 bits (64), Expect = 0.33
 Identities = 13/25 (52%), Positives = 21/25 (84%)
 Frame = +1

Query: 250 RILILGLDGAGKTTILYKLQVGEVV 324
           R+ ILG +GAGK+T++ K+ +GEV+
Sbjct: 721 RVAILGPNGAGKSTLI-KVLIGEVI 744


>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 630

 Score = 28.7 bits (61), Expect = 0.76
 Identities = 12/34 (35%), Positives = 23/34 (67%)
 Frame = +1

Query: 241 REMRILILGLDGAGKTTILYKLQVGEVVTTIPTI 342
           +E+R++I G  G GK++++  L   + VT+IP +
Sbjct: 2   KEVRVVICGDQGVGKSSLISALIQEDNVTSIPKV 35


>SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 198

 Score = 28.7 bits (61), Expect = 0.76
 Identities = 16/59 (27%), Positives = 29/59 (49%), Gaps = 5/59 (8%)
 Frame = +1

Query: 247 MRILILGLDGAGKTTILYKLQVGEV-VTTIPTIGFNVXQVTYK----NLKFQVWDLGGQ 408
           +++ ++G    GKT+++     G     +  T+G N  + T       + F +WDLGGQ
Sbjct: 11  IKVGMIGDSSIGKTSLMVTYVQGSFDEESTQTLGVNFMEKTISIRNTEITFSIWDLGGQ 69


>SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 208

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 20/79 (25%), Positives = 34/79 (43%), Gaps = 5/79 (6%)
 Frame = +1

Query: 247 MRILILGLDGAGKTTILYKLQVGEVVTTI-PTIGFNVXQ----VTYKNLKFQVWDLGGQT 411
           ++++ILG  G GKT ++ +    +       TIG +       V  K +  Q+WD  GQ 
Sbjct: 9   LKVVILGDSGVGKTCLMNQFVNQKFSREYKATIGADFLTKDVVVDDKLVTLQLWDTAGQE 68

Query: 412 SIRPYWRCYYGNTDAIIYV 468
             +     +Y   D  + V
Sbjct: 69  RFQSLGMAFYRGADCCVIV 87


>SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 308

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 23/95 (24%), Positives = 42/95 (44%), Gaps = 10/95 (10%)
 Frame = +1

Query: 250 RILILGLDGAGKTT---ILYKLQVGEVVTTI-PTIGFNVXQVTYK-NLKFQVWDLGGQTS 414
           ++L++G  G+GK++   I++   V +    +  TI      V +  NL   +WD GGQ +
Sbjct: 4   KVLLMGRSGSGKSSMRSIVFSNYVAKDTRRLGATIDIEHSHVRFLGNLVLNLWDCGGQEA 63

Query: 415 IRPYWRC-----YYGNTDAIIYVVXSXDRASNRNI 504
               +        + N   +IYV     R   R++
Sbjct: 64  FMENYLSAQRDHIFRNVQVLIYVFDVESREFERDL 98


>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 407

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 9/26 (34%), Positives = 20/26 (76%)
 Frame = +1

Query: 235 GAREMRILILGLDGAGKTTILYKLQV 312
           G  ++++L+LG   +GKTTI+ ++++
Sbjct: 71  GGNDIKVLLLGAGDSGKTTIMKQMRL 96


>SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 211

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 12/40 (30%), Positives = 18/40 (45%)
 Frame = +1

Query: 373 NLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVXSXDRAS 492
           ++K ++WD  GQ   +     YY N +  I V      AS
Sbjct: 63  SVKLEIWDTAGQERYKSLAPMYYRNANCAIVVYDITQAAS 102


>SPAC23H4.07c |srp102||signal recognition particle receptor beta
           subunit Srp102 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 23/94 (24%), Positives = 46/94 (48%), Gaps = 3/94 (3%)
 Frame = +1

Query: 205 GLFSYFRGLLGAREMRILILGLDGAGKTTILYKLQVGEVVTTIPTIGFNVXQVTYKNLKF 384
           G+F + R  +  +   + ++G   +GKT++  +L   E  TT+P+I  N  +  +K   +
Sbjct: 25  GIF-FTRKTIQKKLPAVFLIGPSDSGKTSLFCELIYKEKKTTVPSIEPN--EAVWKYGAW 81

Query: 385 QVWDLGGQTSIRPYWRCYYG---NTDAIIYVVXS 477
            V DL G    + +    +    N  A+++V+ S
Sbjct: 82  LV-DLPGHPRAKRWITTKFSGNYNVKAVVFVLNS 114


>SPAC4F10.10c |||mannosyltransferase complex subunit, Anp family
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 337

 Score = 25.0 bits (52), Expect = 9.4
 Identities = 12/30 (40%), Positives = 14/30 (46%)
 Frame = +1

Query: 259 ILGLDGAGKTTILYKLQVGEVVTTIPTIGF 348
           I+ LDG G T +L K  V       PT  F
Sbjct: 275 IMALDGVGTTALLVKASVHRDGALFPTFPF 304


>SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1573

 Score = 25.0 bits (52), Expect = 9.4
 Identities = 9/19 (47%), Positives = 15/19 (78%)
 Frame = +1

Query: 238 AREMRILILGLDGAGKTTI 294
           ++   I+++G+ GAGKTTI
Sbjct: 861 SKNASIILIGMRGAGKTTI 879


>SPAC2G11.13 |atg22||autophagy associated protein Atg22
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 529

 Score = 25.0 bits (52), Expect = 9.4
 Identities = 11/34 (32%), Positives = 20/34 (58%)
 Frame = -1

Query: 383 NLRFLYVTCXTLNPIVGIVVTTSPTCNL*RIVVF 282
           N R++  +C  +  I GI+++ SP+  L   +VF
Sbjct: 113 NRRYILFSCVIIGSISGIILSWSPSSFLLGKIVF 146


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,599,118
Number of Sequences: 5004
Number of extensions: 52265
Number of successful extensions: 136
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -