BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_D02
(652 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55270.1 68416.m06138 MAP kinase phosphatase (MKP1) identical... 31 0.66
At3g19830.1 68416.m02512 C2 domain-containing protein low simila... 29 2.0
At4g16630.1 68417.m02514 DEAD/DEAH box helicase, putative (RH28)... 29 2.7
At2g07727.1 68415.m00977 cytochrome b (MTCYB) (COB) (CYTB) conta... 29 2.7
At2g37290.1 68415.m04574 RabGAP/TBC domain-containing protein lo... 28 4.7
At3g46550.1 68416.m05053 fasciclin-like arabinogalactan family p... 28 6.2
At5g51540.1 68418.m06391 peptidase M3 family protein / thimet ol... 27 8.2
At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) simil... 27 8.2
>At3g55270.1 68416.m06138 MAP kinase phosphatase (MKP1) identical to
MAP kinase phosphatase (MKP1) GI:13540262 from
[Arabidopsis thaliana]
Length = 534
Score = 31.1 bits (67), Expect = 0.66
Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 4/52 (7%)
Frame = +1
Query: 13 PTRGRSARPYSRPRPTRRA-PAMFDVFGSVKGLLKLDSVCIDNN---VFRLH 156
P R + +P P R+A P++ + GS+KG LKL + N F LH
Sbjct: 309 PKESRGVNTFLQPSPNRKASPSLAERRGSLKGSLKLPGLADSNRGTPAFTLH 360
>At3g19830.1 68416.m02512 C2 domain-containing protein low
similarity to GLUT4 vesicle protein [Rattus norvegicus]
GI:4193489; contains Pfam profile PF00168: C2 domain
Length = 666
Score = 29.5 bits (63), Expect = 2.0
Identities = 21/79 (26%), Positives = 36/79 (45%), Gaps = 1/79 (1%)
Frame = -2
Query: 387 FNFVLSFDMWADAGLHVILSDAADKSVRDGESRVYPAVRVHNGERDF-INDAVNRVTDVL 211
F VLS + ++ ++ S+ + V DGES V P + + + DA N L
Sbjct: 563 FMNVLSALILSEEFQGIVSSETGNNKVDDGESSVSPVPSMSGADSESRPKDAGNGDVSDL 622
Query: 210 SRRDEKRERDQDNHGGFVM 154
++ K +R N+GG +
Sbjct: 623 EVKNAKSDRGSINNGGLAL 641
>At4g16630.1 68417.m02514 DEAD/DEAH box helicase, putative (RH28)
identical to cDNA DEAD box RNA helicase, RH28 GI:3776026
Length = 789
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = -2
Query: 327 DAADKSVRDGESRVYPAVRVHNGERDFINDAVNRVTDVLSRRDEKRERDQD 175
D++++ V++GE+ + A +GE ++ + D DEKR+RD D
Sbjct: 23 DSSEEDVKEGEAEEHEAGEDEDGEEEYEEE-----DDDEEEEDEKRKRDAD 68
>At2g07727.1 68415.m00977 cytochrome b (MTCYB) (COB) (CYTB) contains
Pfam profile PF00033: Cytochrome b(N-terminal)/b6/petB;
ontains Pfam profile PF00032: Cytochrome
b(C-terminal)/b6/petD; 99% identical to apocytochrome B
(GI:6851014), cytochrome b (GI:402962), and Cytochrome b
(Swiss-Prot:P42792) [Arabidopsis thaliana]
Length = 393
Score = 29.1 bits (62), Expect = 2.7
Identities = 14/43 (32%), Positives = 22/43 (51%), Gaps = 6/43 (13%)
Frame = +1
Query: 352 VGPHVEGQDEVKYHKYYQ------WVCFVLFFQAILFYVPRYL 462
+G H E D++ ++ Y+ WV F +FF +FY P L
Sbjct: 216 LGVHSE-MDKIAFYPYFYVKDLVGWVAFAIFFSIWIFYAPNVL 257
>At2g37290.1 68415.m04574 RabGAP/TBC domain-containing protein low
similarity to Rab6 GTPase activating protein, GAPCenA
[Homo sapiens] GI:12188746; contains Pfam profile
PF00566: TBC domain
Length = 882
Score = 28.3 bits (60), Expect = 4.7
Identities = 12/41 (29%), Positives = 23/41 (56%)
Frame = -2
Query: 294 SRVYPAVRVHNGERDFINDAVNRVTDVLSRRDEKRERDQDN 172
SRV NG+++ ++D + + + LS +E E D+D+
Sbjct: 238 SRVKNVKSTKNGQKNIVDDHASSIKESLSSIEESGENDRDS 278
>At3g46550.1 68416.m05053 fasciclin-like arabinogalactan family
protein similar to fasciclin-like arabinogalactan
protein FLA8 [Arabidopsis thaliana]
gi|10880493|gb|AAG24276
Length = 420
Score = 27.9 bits (59), Expect = 6.2
Identities = 16/54 (29%), Positives = 30/54 (55%)
Frame = +2
Query: 242 LMKSRSPLWTRTAGYTLLSPSRTDLSAASERITCNPASAHMSKDKTKLNITNII 403
L++++ P T +L+ P+ D++A SE +T P S +S +N+T I+
Sbjct: 160 LLETKPPNITVLTVDSLIVPTGIDITA-SETLTPPPTSTSLSPPPAGINLTQIL 212
>At5g51540.1 68418.m06391 peptidase M3 family protein / thimet
oligopeptidase family protein low similarity to
SP|Q99797 Mitochondrial intermediate peptidase,
mitochondrial precursor (EC 3.4.24.59) {Homo sapiens};
contains Pfam profile PF01432: Peptidase family M3
Length = 860
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/58 (24%), Positives = 29/58 (50%)
Frame = +1
Query: 61 RRAPAMFDVFGSVKGLLKLDSVCIDNNVFRLHYKATVIILIAFSLLVTSRQYIGDPID 234
RRAP VF S +G K + + N F L + I+++ +++ + ++ + +D
Sbjct: 763 RRAPVEKPVFMSEEGAAKAEEQRQNENAFLLTWLGLGIVILIEGIILAASGFLPEELD 820
>At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) similar
to sodium/calcium exchanger protein [Mus musculus]
gi|13925661|gb|AAK49407; Ca2+:Cation Antiporter (CaCA)
Family member PMID:11500563
Length = 559
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/62 (24%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
Frame = +1
Query: 283 IYSTFTIPNRLIGRVGKDYVQPGV--GPHVEGQDEVKYHKYYQWVCFVLFFQAILFYVPR 456
I+ ++ ++ R G + ++ GV G H D+ + +YY W V++ + +PR
Sbjct: 260 IHKRGSLSEPILQRDGLEEIEDGVVNGEHQIVDDDDDHQRYYYWKRLVIWAITLPLNLPR 319
Query: 457 YL 462
L
Sbjct: 320 IL 321
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,613,985
Number of Sequences: 28952
Number of extensions: 316096
Number of successful extensions: 981
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 942
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 980
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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