BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_C20 (637 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC15F9.03c |nxt2|nft2, ntf2, ntf2, nft2, SPAC1B9.01c|nuclear t... 48 1e-06 SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyc... 47 3e-06 SPBC32F12.12c |||conserved fungal protein|Schizosaccharomyces po... 26 4.0 SPCC16C4.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 26 5.2 SPBC56F2.05c |||transcription factor |Schizosaccharomyces pombe|... 25 6.9 SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 9.1 >SPAC15F9.03c |nxt2|nft2, ntf2, ntf2, nft2, SPAC1B9.01c|nuclear transport factor Nxt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 123 Score = 48.0 bits (109), Expect = 1e-06 Identities = 28/72 (38%), Positives = 41/72 (56%), Gaps = 2/72 (2%) Frame = +1 Query: 256 IMEKLNSLTFQKITXIVTAVDSQPM-FDGGVLINVLGRLKCDEXP-PHLYMQTFVLXPLG 429 I+EKL SL FQ++ ++ +D+QP G V++ V G L DE Y Q F L Sbjct: 49 IVEKLVSLPFQRVQHRISTLDAQPTGTTGSVIVMVTGELLLDEEQMAQRYSQVFHLVNNN 108 Query: 430 XSFYVQHDIFRL 465 ++YV +D+FRL Sbjct: 109 GNYYVLNDLFRL 120 Score = 26.6 bits (56), Expect = 3.0 Identities = 12/26 (46%), Positives = 18/26 (69%) Frame = +3 Query: 168 AQRANLVNMYNVETSFMTFEGVQLQG 245 + R+ L ++Y E S ++FEG QLQG Sbjct: 21 SDRSQLSSLYR-EESMLSFEGAQLQG 45 >SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyces pombe|chr 2|||Manual Length = 434 Score = 46.8 bits (106), Expect = 3e-06 Identities = 25/81 (30%), Positives = 39/81 (48%) Frame = +1 Query: 238 CRAAVKIMEKLNSLTFQKITXIVTAVDSQPMFDGGVLINVLGRLKCDEXPPHLYMQTFVL 417 C +I K+ L FQ +++ VDS +GG++I VLG + + QTF L Sbjct: 59 CHGQQEIHNKILDLDFQNCKVLISNVDSLASSNGGIVIQVLGEMSNKGKLSRKFAQTFFL 118 Query: 418 XPLGXSFYVQHDIFRLGIHDI 480 ++V +DIFR D+ Sbjct: 119 AEQPNGYFVLNDIFRFLREDV 139 >SPBC32F12.12c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 164 Score = 26.2 bits (55), Expect = 4.0 Identities = 18/66 (27%), Positives = 36/66 (54%), Gaps = 2/66 (3%) Frame = -3 Query: 311 AVTIXVIF*KVKLFNFS-IILTAALQLYSLKGHE*SF-NIVHINKICPLSXDHRTMCNIV 138 ++ + +I V LF+ + ++L +AL + ++G F + +++ICP+S + N Sbjct: 34 SIFLCIILGIVNLFHVTLVVLFSALTI--IEGVLLIFIELPFLSRICPVSDKFQAFTNAF 91 Query: 137 AQNLYR 120 A N YR Sbjct: 92 ASNYYR 97 >SPCC16C4.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 266 Score = 25.8 bits (54), Expect = 5.2 Identities = 10/22 (45%), Positives = 17/22 (77%) Frame = -3 Query: 92 FRLRCVYLSHQQKAQFQPEKFK 27 FRL+ Y+SH +K+ F P+++K Sbjct: 235 FRLK-KYISHSKKSAFSPQRWK 255 >SPBC56F2.05c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 397 Score = 25.4 bits (53), Expect = 6.9 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = -2 Query: 261 HNFNSSPAVVLPQRS*MKFQHCTY*QDLP 175 H+F++ V +PQ+S Q C+Y + LP Sbjct: 116 HSFSNPRYVAVPQKSTSPNQVCSYCEPLP 144 >SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 796 Score = 25.0 bits (52), Expect = 9.1 Identities = 10/33 (30%), Positives = 19/33 (57%) Frame = -3 Query: 323 WESTAVTIXVIF*KVKLFNFSIILTAALQLYSL 225 W ST + + F + +NFS ++ +++Q SL Sbjct: 718 WLSTVIFPLLCFTVISAYNFSTMIRSSMQFVSL 750 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,433,382 Number of Sequences: 5004 Number of extensions: 45102 Number of successful extensions: 81 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 77 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 80 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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