Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_C08
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g80460.1 68414.m09423 glycerol kinase, putative similar to gl... 33 0.22
At5g58470.2 68418.m07323 zinc finger (Ran-binding) family protei... 32 0.29
At5g58470.1 68418.m07322 zinc finger (Ran-binding) family protei... 32 0.29
At5g19090.1 68418.m02269 heavy-metal-associated domain-containin... 30 1.2
At4g18375.2 68417.m02727 KH domain-containing protein contains s... 29 3.5
At4g18375.1 68417.m02726 KH domain-containing protein contains s... 29 3.5
At1g44910.1 68414.m05146 FF domain-containing protein / WW domai... 29 3.5
At5g58010.1 68418.m07258 basic helix-loop-helix (bHLH) family pr... 28 4.7
At2g32930.1 68415.m04037 zinc finger (CCCH-type) family protein ... 28 4.7
At2g45850.2 68415.m05703 DNA-binding family protein contains a A... 28 6.1
At2g45850.1 68415.m05702 DNA-binding family protein contains a A... 28 6.1
At5g24930.1 68418.m02952 zinc finger (B-box type) family protein... 27 8.1
At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes... 27 8.1
>At1g80460.1 68414.m09423 glycerol kinase, putative similar to
glycerol kinase (ATP:glycerol 3-phosphotransferase,
Glycerokinase, GK)[Mycobacterium tuberculosis]
Swiss-Prot:O69664
Length = 522
Score = 32.7 bits (71), Expect = 0.22
Identities = 20/57 (35%), Positives = 27/57 (47%)
Frame = +3
Query: 153 MPAQPYPNLYSNNMVAGGSCMGSPSVGYSPPSTMASCMGGAGAVPYGSLPREQEAAS 323
+PA+ P + SN+ V G C G P G +A C+G A G R+ EA S
Sbjct: 220 IPAEILPKIVSNSEVIGEICKGWPIPGIK----IAGCLGDQHAAMLGQACRKGEAKS 272
>At5g58470.2 68418.m07323 zinc finger (Ran-binding) family protein
weak similarity to SP|Q01844 RNA-binding protein EWS
(EWS oncogene) (Ewing sarcoma breakpoint region 1
protein) {Homo sapiens}; contains Pfam profiles PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF00641: Zn-finger in Ran binding protein and others
Length = 422
Score = 32.3 bits (70), Expect = 0.29
Identities = 22/67 (32%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
Frame = +3
Query: 153 MPAQPYPNLYSNNMVAGGSCMGSPSVGYSPPSTMASCMGG-AGAVPYGSLPREQEAASPT 329
M A P P YS G G P GY + GG +G GS PR QEA+
Sbjct: 205 MEAVPPPTSYSG----GPPSYGGPRGGYGSDAPSTGGRGGRSGGYDGGSAPRRQEASYED 260
Query: 330 SALQRAR 350
+A ++ +
Sbjct: 261 AATEKVK 267
>At5g58470.1 68418.m07322 zinc finger (Ran-binding) family protein
weak similarity to SP|Q01844 RNA-binding protein EWS
(EWS oncogene) (Ewing sarcoma breakpoint region 1
protein) {Homo sapiens}; contains Pfam profiles PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF00641: Zn-finger in Ran binding protein and others
Length = 422
Score = 32.3 bits (70), Expect = 0.29
Identities = 22/67 (32%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
Frame = +3
Query: 153 MPAQPYPNLYSNNMVAGGSCMGSPSVGYSPPSTMASCMGG-AGAVPYGSLPREQEAASPT 329
M A P P YS G G P GY + GG +G GS PR QEA+
Sbjct: 205 MEAVPPPTSYSG----GPPSYGGPRGGYGSDAPSTGGRGGRSGGYDGGSAPRRQEASYED 260
Query: 330 SALQRAR 350
+A ++ +
Sbjct: 261 AATEKVK 267
>At5g19090.1 68418.m02269 heavy-metal-associated domain-containing
protein contains Pfam heavy-metal-associated domain
PF00403; glycine-rich protein GRP22, rape, PIR:S31415;
isoform contains a non-consensus TG-acceptor splice site
at intron 3
Length = 587
Score = 30.3 bits (65), Expect = 1.2
Identities = 18/50 (36%), Positives = 22/50 (44%)
Frame = +3
Query: 183 SNNMVAGGSCMGSPSVGYSPPSTMASCMGGAGAVPYGSLPREQEAASPTS 332
S + G MG G P +M+ MGGA P GSLP+ P S
Sbjct: 399 SGGLPPGFRPMGGGGGGGGGPQSMSMPMGGAMGGPMGSLPQMGGGPGPMS 448
>At4g18375.2 68417.m02727 KH domain-containing protein contains
similarity to RNA-binding KH-domains PF:00013
Length = 606
Score = 28.7 bits (61), Expect = 3.5
Identities = 15/45 (33%), Positives = 19/45 (42%)
Frame = +3
Query: 216 GSPSVGYSPPSTMASCMGGAGAVPYGSLPREQEAASPTSALQRAR 350
GS + GY+ PS M+S +G YGS P T R
Sbjct: 487 GSSNAGYTLPSFMSSMASTSGFHGYGSFPAGDNVLGSTGPYSYGR 531
>At4g18375.1 68417.m02726 KH domain-containing protein contains
similarity to RNA-binding KH-domains PF:00013
Length = 532
Score = 28.7 bits (61), Expect = 3.5
Identities = 15/45 (33%), Positives = 19/45 (42%)
Frame = +3
Query: 216 GSPSVGYSPPSTMASCMGGAGAVPYGSLPREQEAASPTSALQRAR 350
GS + GY+ PS M+S +G YGS P T R
Sbjct: 487 GSSNAGYTLPSFMSSMASTSGFHGYGSFPAGDNVLGSTGPYSYGR 531
>At1g44910.1 68414.m05146 FF domain-containing protein / WW
domain-containing protein contains Pfam profiles
PF01846: FF domain, PF00397: WW domain
Length = 946
Score = 28.7 bits (61), Expect = 3.5
Identities = 16/57 (28%), Positives = 26/57 (45%)
Frame = +3
Query: 171 PNLYSNNMVAGGSCMGSPSVGYSPPSTMASCMGGAGAVPYGSLPREQEAASPTSALQ 341
P + +N ++ GS P+ +PP T + G + PY +P PTS +Q
Sbjct: 71 PYIQTNKILTSGSTQPQPN---APPMTGFATSGPPFSSPYTFVPSSYPQQQPTSLVQ 124
>At5g58010.1 68418.m07258 basic helix-loop-helix (bHLH) family
protein bHLH transcription factor GBOF-1, Tulipa
gesneriana, EMBL:AF185269; contains Pfam profile
PF00010: Helix-loop-helix DNA-binding domain
Length = 297
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/69 (24%), Positives = 29/69 (42%)
Frame = +3
Query: 165 PYPNLYSNNMVAGGSCMGSPSVGYSPPSTMASCMGGAGAVPYGSLPREQEAASPTSALQR 344
P+P+ + + +GG MGS G P T+++ R +A P S +R
Sbjct: 57 PFPDQFHHPQESGGPTMGSQE-GLQPQGTVSTTSAPVVRQKPRVRARRGQATDPHSIAER 115
Query: 345 ARNDKTYRR 371
R ++ R
Sbjct: 116 LRRERIAER 124
>At2g32930.1 68415.m04037 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 453
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/57 (29%), Positives = 26/57 (45%)
Frame = +3
Query: 141 GMGCMPAQPYPNLYSNNMVAGGSCMGSPSVGYSPPSTMASCMGGAGAVPYGSLPREQ 311
G+ C P+P+ +N + + PSVG+ S M M YG++PR Q
Sbjct: 147 GVACKFHHPHPHSQPSNGHSAYAMSSFPSVGFPYASGMT--MVSLPPATYGAIPRPQ 201
>At2g45850.2 68415.m05703 DNA-binding family protein contains a AT
hook motif (DNA binding motifs with a preference for A/T
rich regions), Pfam:PF02178
Length = 348
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = +3
Query: 198 AGGSCMGSPSVGYSPPSTMASCMGGAGAVPY 290
+G + GSPS+ P ++A+ GGAGA+P+
Sbjct: 51 SGSTGFGSPSLHGDP--SLATAAGGAGALPH 79
>At2g45850.1 68415.m05702 DNA-binding family protein contains a AT
hook motif (DNA binding motifs with a preference for A/T
rich regions), Pfam:PF02178
Length = 348
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = +3
Query: 198 AGGSCMGSPSVGYSPPSTMASCMGGAGAVPY 290
+G + GSPS+ P ++A+ GGAGA+P+
Sbjct: 51 SGSTGFGSPSLHGDP--SLATAAGGAGALPH 79
>At5g24930.1 68418.m02952 zinc finger (B-box type) family protein
similar to CONSTANS-like protein 1 GI:4091804 from
[Malus x domestica]
Length = 406
Score = 27.5 bits (58), Expect = 8.1
Identities = 21/91 (23%), Positives = 33/91 (36%), Gaps = 4/91 (4%)
Frame = +3
Query: 132 YVNGMGCMPAQPYPNLYSNNMVAGGSCMGSPSVGYSPPSTMASCMGGAGAVPYGSLPREQ 311
Y G C+ + +V G + S Y P+T + G AVP S RE
Sbjct: 282 YGGGYNCISHSVSSSSMEVGVVPDGGSVADVSYPYGGPATSGADPGTQRAVPLTSAEREA 341
Query: 312 EAASPTSALQRARNDKTY----RRSYTHAKP 392
+ + +KT R++Y +P
Sbjct: 342 RVMRYREKRKNRKFEKTIRYASRKAYAEMRP 372
>At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes
from this gene
Length = 1035
Score = 27.5 bits (58), Expect = 8.1
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +3
Query: 267 GGAGAVPYGSLPREQEAASPTSALQRARNDKTYRRS 374
G + +GSL R++ +PTS ++RA K +RS
Sbjct: 86 GSSNGSGFGSLGRKEVLTTPTSRVKRALGLKMLKRS 121
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,584,014
Number of Sequences: 28952
Number of extensions: 245453
Number of successful extensions: 819
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 789
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 819
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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