BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_C01 (650 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 41 1e-04 SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 40 3e-04 SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces po... 40 3e-04 SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 34 0.020 SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 33 0.047 SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 25 7.2 SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 25 9.5 >SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 259 Score = 41.1 bits (92), Expect = 1e-04 Identities = 37/148 (25%), Positives = 61/148 (41%), Gaps = 3/148 (2%) Frame = +2 Query: 161 KLAGRTLFITGASRXXXXXXXXXXXXXXX-NVVIAAKTAEPHPKLPGTIYTAAEEIEA-L 334 +L G+T+ ITGAS +++AA+ T+ A+E+E+ Sbjct: 3 RLDGKTILITGASSGIGKSTAFEIAKVAKVKLILAARRFS-------TVEEIAKELESKY 55 Query: 335 GGKALPCIVDVXXEKQVXKAVDEAVKKFNGIDILXNNAS-AISLTGTAQTDMKRYDLMHN 511 LP +DV K + ++ K+F ID+L NNA A+ ++ M Sbjct: 56 EVSVLPLKLDVSDLKSIPGVIESLPKEFADIDVLINNAGLALGTDKVIDLNIDDAVTMIT 115 Query: 512 INTXGTFLASXICLPVLKNSNHAHILNL 595 N G + LP+ + N ILN+ Sbjct: 116 TNVLGMMAMTRAVLPIFYSKNKGDILNV 143 >SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 236 Score = 39.9 bits (89), Expect = 3e-04 Identities = 20/80 (25%), Positives = 38/80 (47%) Frame = +2 Query: 359 VDVXXEKQVXKAVDEAVKKFNGIDILXNNASAISLTGTAQTDMKRYDLMHNINTXGTFLA 538 +DV + K+V++A+ F ID+L NNA + T ++ + N N G Sbjct: 21 LDVNNFSSIKKSVEKAISHFGRIDVLLNNAGYSVYSPLESTTEEQIHNIFNTNVFGALEV 80 Query: 539 SXICLPVLKNSNHAHILNLS 598 P+ ++ ++ I+N+S Sbjct: 81 IKAITPIFRSQHNGMIINVS 100 >SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 236 Score = 39.9 bits (89), Expect = 3e-04 Identities = 20/80 (25%), Positives = 38/80 (47%) Frame = +2 Query: 359 VDVXXEKQVXKAVDEAVKKFNGIDILXNNASAISLTGTAQTDMKRYDLMHNINTXGTFLA 538 +DV + K+V++A+ F ID+L NNA + T ++ + N N G Sbjct: 21 LDVNNFSSIKKSVEKAISHFGRIDVLLNNAGYSVYSPLESTTEEQIHNIFNTNVFGALEV 80 Query: 539 SXICLPVLKNSNHAHILNLS 598 P+ ++ ++ I+N+S Sbjct: 81 IKAITPIFRSQHNGMIINVS 100 >SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 33.9 bits (74), Expect = 0.020 Identities = 21/85 (24%), Positives = 37/85 (43%), Gaps = 1/85 (1%) Frame = +2 Query: 344 ALPCIVDVXXEKQVXKAVDEAVKKFNGIDILXNNASAISLTGTAQTDMKRYDLMHNINTX 523 AL DV Q+ A+++ ++ ID+L NNA T Q + +D ++ Sbjct: 47 ALALQADVSKADQIETAIEKVIQTLGPIDVLINNAGLADDTPFEQLSHESWDHDVSLVLR 106 Query: 524 GTFLASXICLP-VLKNSNHAHILNL 595 G +L +P + K I+N+ Sbjct: 107 GNYLTQRYVIPHMAKQGKGGSIVNI 131 >SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 292 Score = 32.7 bits (71), Expect = 0.047 Identities = 26/98 (26%), Positives = 44/98 (44%), Gaps = 2/98 (2%) Frame = +2 Query: 311 AAEEIEALGGKALPCIVDVXXEKQVXKAVDEAVKKFNGIDILXNNASAISLTGTAQT-DM 487 A + I K L +DV K V A +A ++F +DI+ NNA L G ++ ++ Sbjct: 38 APDTITIEHSKLLKLKLDVTDVKSVETAFKDAKRRFGNVDIVINNA-GYGLVGEFESYNI 96 Query: 488 KRYDLMHNINTXGTFLASXICLPVLKNSNH-AHILNLS 598 + N+N G + L +++ S IL +S Sbjct: 97 EEMHRQMNVNFWGVAYITKEALNLMRESGKGGRILQIS 134 >SPBC36.10 |||mitochondrial intermembrane space protein sorting protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 25.4 bits (53), Expect = 7.2 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = +2 Query: 269 TAEPHPKLPGTIYTAAEEIEAL 334 T PHP+L T++ IEAL Sbjct: 110 TYSPHPELEATVFQQEARIEAL 131 >SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 629 Score = 25.0 bits (52), Expect = 9.5 Identities = 18/56 (32%), Positives = 24/56 (42%), Gaps = 1/56 (1%) Frame = -3 Query: 429 SMPLNFFTASSTAXCTCFSXXTSTMHGNAFPPRASISSAAV*IVPG-SLG*GSAVF 265 S +F S TCFS + + +PP ASI + I P G GS +F Sbjct: 8 SCSFSFVVFVSVLLQTCFSFQLTPLSPKNYPPGASIDTTVNTISPFIGDGRGSDIF 63 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,282,920 Number of Sequences: 5004 Number of extensions: 39141 Number of successful extensions: 77 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 74 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 76 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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