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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_B04
         (652 letters)

Database: mosquito 
           2352 sequences; 563,979 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY578799-1|AAT07304.1|  679|Anopheles gambiae brinker protein.         90   5e-20
AY705398-1|AAU12507.1|  555|Anopheles gambiae nicotinic acetylch...    25   2.7  
AY705397-1|AAU12506.1|  555|Anopheles gambiae nicotinic acetylch...    25   2.7  
AJ439353-1|CAD27923.1| 1127|Anopheles gambiae putative Na-K-Cl s...    23   8.4  
AJ010903-1|CAA09389.1|  373|Anopheles gambiae ICHIT protein prot...    23   8.4  

>AY578799-1|AAT07304.1|  679|Anopheles gambiae brinker protein.
          Length = 679

 Score = 90.2 bits (214), Expect = 5e-20
 Identities = 39/51 (76%), Positives = 44/51 (86%)
 Frame = +3

Query: 120 RAGSRRIFPPQFKLQVLEAYRRDSQCRGNQRATARKFGIHRRQIQKWLQAE 272
           + GSRRIF  QFKLQVL++YR D  C+GNQRATARK+GIHRRQIQKWLQ E
Sbjct: 43  KMGSRRIFTAQFKLQVLDSYRNDGDCKGNQRATARKYGIHRRQIQKWLQVE 93



 Score = 44.8 bits (101), Expect = 2e-06
 Identities = 17/41 (41%), Positives = 27/41 (65%)
 Frame = +3

Query: 150 QFKLQVLEAYRRDSQCRGNQRATARKFGIHRRQIQKWLQAE 272
           + K Q ++++  D  CRG+ RA A K+ I R+ ++KWLQ E
Sbjct: 603 KLKRQAIDSFYHDVACRGDYRAVATKYNISRKYVEKWLQQE 643



 Score = 29.1 bits (62), Expect = 0.13
 Identities = 14/41 (34%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
 Frame = +3

Query: 468 SVRRCSPXTLAHPPVAPEP--PRKPFKLFRPYLLEDEDEKR 584
           S  + S   ++ P   P+   P K  KLF+PYLL++E +++
Sbjct: 431 SSNKRSSSAMSGPIATPDTSRPAKSVKLFKPYLLDEEPKQQ 471


>AY705398-1|AAU12507.1|  555|Anopheles gambiae nicotinic
           acetylcholine receptor subunitalpha 4 protein.
          Length = 555

 Score = 24.6 bits (51), Expect = 2.7
 Identities = 19/46 (41%), Positives = 25/46 (54%)
 Frame = -2

Query: 441 VPMLEQVEV*AMKLTLAS*EVEVV*VILHFRGNQQHMEEVMAPAVE 304
           VP+L +  + AM L   S  V VV + +HFR  Q H    MAP V+
Sbjct: 303 VPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHR---MAPWVK 345


>AY705397-1|AAU12506.1|  555|Anopheles gambiae nicotinic
           acetylcholine receptor subunitalpha 4 protein.
          Length = 555

 Score = 24.6 bits (51), Expect = 2.7
 Identities = 19/46 (41%), Positives = 25/46 (54%)
 Frame = -2

Query: 441 VPMLEQVEV*AMKLTLAS*EVEVV*VILHFRGNQQHMEEVMAPAVE 304
           VP+L +  + AM L   S  V VV + +HFR  Q H    MAP V+
Sbjct: 303 VPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHR---MAPWVK 345


>AJ439353-1|CAD27923.1| 1127|Anopheles gambiae putative Na-K-Cl
            symporter protein.
          Length = 1127

 Score = 23.0 bits (47), Expect = 8.4
 Identities = 10/28 (35%), Positives = 15/28 (53%)
 Frame = +3

Query: 549  RPYLLEDEDEKRXXIASLPVSNTGYVSA 632
            R YLL+   +    + +LP+   G VSA
Sbjct: 1068 REYLLQHSSKSDLVVMTLPMPRKGVVSA 1095


>AJ010903-1|CAA09389.1|  373|Anopheles gambiae ICHIT protein
           protein.
          Length = 373

 Score = 23.0 bits (47), Expect = 8.4
 Identities = 8/15 (53%), Positives = 9/15 (60%)
 Frame = +3

Query: 492 TLAHPPVAPEPPRKP 536
           T A+PP   EPP  P
Sbjct: 268 TTAYPPTTSEPPSTP 282


  Database: mosquito
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 563,979
  Number of sequences in database:  2352
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 565,327
Number of Sequences: 2352
Number of extensions: 9243
Number of successful extensions: 27
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27
length of database: 563,979
effective HSP length: 62
effective length of database: 418,155
effective search space used: 64395870
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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