BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_B04 (652 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AY578799-1|AAT07304.1| 679|Anopheles gambiae brinker protein. 90 5e-20 AY705398-1|AAU12507.1| 555|Anopheles gambiae nicotinic acetylch... 25 2.7 AY705397-1|AAU12506.1| 555|Anopheles gambiae nicotinic acetylch... 25 2.7 AJ439353-1|CAD27923.1| 1127|Anopheles gambiae putative Na-K-Cl s... 23 8.4 AJ010903-1|CAA09389.1| 373|Anopheles gambiae ICHIT protein prot... 23 8.4 >AY578799-1|AAT07304.1| 679|Anopheles gambiae brinker protein. Length = 679 Score = 90.2 bits (214), Expect = 5e-20 Identities = 39/51 (76%), Positives = 44/51 (86%) Frame = +3 Query: 120 RAGSRRIFPPQFKLQVLEAYRRDSQCRGNQRATARKFGIHRRQIQKWLQAE 272 + GSRRIF QFKLQVL++YR D C+GNQRATARK+GIHRRQIQKWLQ E Sbjct: 43 KMGSRRIFTAQFKLQVLDSYRNDGDCKGNQRATARKYGIHRRQIQKWLQVE 93 Score = 44.8 bits (101), Expect = 2e-06 Identities = 17/41 (41%), Positives = 27/41 (65%) Frame = +3 Query: 150 QFKLQVLEAYRRDSQCRGNQRATARKFGIHRRQIQKWLQAE 272 + K Q ++++ D CRG+ RA A K+ I R+ ++KWLQ E Sbjct: 603 KLKRQAIDSFYHDVACRGDYRAVATKYNISRKYVEKWLQQE 643 Score = 29.1 bits (62), Expect = 0.13 Identities = 14/41 (34%), Positives = 24/41 (58%), Gaps = 2/41 (4%) Frame = +3 Query: 468 SVRRCSPXTLAHPPVAPEP--PRKPFKLFRPYLLEDEDEKR 584 S + S ++ P P+ P K KLF+PYLL++E +++ Sbjct: 431 SSNKRSSSAMSGPIATPDTSRPAKSVKLFKPYLLDEEPKQQ 471 >AY705398-1|AAU12507.1| 555|Anopheles gambiae nicotinic acetylcholine receptor subunitalpha 4 protein. Length = 555 Score = 24.6 bits (51), Expect = 2.7 Identities = 19/46 (41%), Positives = 25/46 (54%) Frame = -2 Query: 441 VPMLEQVEV*AMKLTLAS*EVEVV*VILHFRGNQQHMEEVMAPAVE 304 VP+L + + AM L S V VV + +HFR Q H MAP V+ Sbjct: 303 VPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHR---MAPWVK 345 >AY705397-1|AAU12506.1| 555|Anopheles gambiae nicotinic acetylcholine receptor subunitalpha 4 protein. Length = 555 Score = 24.6 bits (51), Expect = 2.7 Identities = 19/46 (41%), Positives = 25/46 (54%) Frame = -2 Query: 441 VPMLEQVEV*AMKLTLAS*EVEVV*VILHFRGNQQHMEEVMAPAVE 304 VP+L + + AM L S V VV + +HFR Q H MAP V+ Sbjct: 303 VPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHR---MAPWVK 345 >AJ439353-1|CAD27923.1| 1127|Anopheles gambiae putative Na-K-Cl symporter protein. Length = 1127 Score = 23.0 bits (47), Expect = 8.4 Identities = 10/28 (35%), Positives = 15/28 (53%) Frame = +3 Query: 549 RPYLLEDEDEKRXXIASLPVSNTGYVSA 632 R YLL+ + + +LP+ G VSA Sbjct: 1068 REYLLQHSSKSDLVVMTLPMPRKGVVSA 1095 >AJ010903-1|CAA09389.1| 373|Anopheles gambiae ICHIT protein protein. Length = 373 Score = 23.0 bits (47), Expect = 8.4 Identities = 8/15 (53%), Positives = 9/15 (60%) Frame = +3 Query: 492 TLAHPPVAPEPPRKP 536 T A+PP EPP P Sbjct: 268 TTAYPPTTSEPPSTP 282 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 565,327 Number of Sequences: 2352 Number of extensions: 9243 Number of successful extensions: 27 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 25 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 27 length of database: 563,979 effective HSP length: 62 effective length of database: 418,155 effective search space used: 64395870 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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