BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_B03 (653 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g33560.1 68415.m04113 spindle checkpoint protein-related simi... 70 1e-12 At5g05510.1 68418.m00598 protein kinase-related low similarity t... 35 0.054 At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)... 27 8.2 At2g34150.1 68415.m04180 expressed protein 27 8.2 >At2g33560.1 68415.m04113 spindle checkpoint protein-related similar to spindle checkpoint protein BubR1 (GI:22128593) [Xenopus laevis]; similar to Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (EC 2.7.1.-) (MAD3/BUB1-related protein kinase) (Mitotic checkpoint kinase MAD3L) (Swiss-Prot:Q9Z1S0) [Mus musculus] Length = 395 Score = 70.1 bits (164), Expect = 1e-12 Identities = 32/95 (33%), Positives = 55/95 (57%) Frame = +2 Query: 365 DFXKENIQPLRGGRNLVQLSTALQAQSDADAQKQLQIQKEEHETAIXNYQXPDPLEPWFN 544 + KEN++PL+ GRN+ L+ AL++ SD +K L ++ AI Y+ DPL PW Sbjct: 28 ELFKENVRPLKRGRNVGILNHALKSHSDHQLRKNLIEKRRNLIEAIDEYEGDDPLSPWIE 87 Query: 545 YIQWVEQSFPKHGHEXHIDKLIKXCLQLFENDNKY 649 I+WV+++FP G + + + C++ F + +Y Sbjct: 88 CIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERY 122 >At5g05510.1 68418.m00598 protein kinase-related low similarity to SP|O60566 Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (EC 2.7.1.-) {Homo sapiens} Length = 471 Score = 34.7 bits (76), Expect = 0.054 Identities = 13/50 (26%), Positives = 26/50 (52%) Frame = +2 Query: 500 IXNYQXPDPLEPWFNYIQWVEQSFPKHGHEXHIDKLIKXCLQLFENDNKY 649 I +Y D L PW ++ +++S P + + ++ C + FE+D +Y Sbjct: 19 IKSYSGKDHLLPWIRGVKKMKESLPSQILNEKLPRFLQKCAESFESDKRY 68 >At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V) family protein similar to SP|P11931 Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (VALRS) {Bacillus stearothermophilus}; contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and V) Length = 970 Score = 27.5 bits (58), Expect = 8.2 Identities = 11/34 (32%), Positives = 19/34 (55%) Frame = +2 Query: 455 AQKQLQIQKEEHETAIXNYQXPDPLEPWFNYIQW 556 A++ L+ E++ + YQ PD L+ WF+ W Sbjct: 494 AEEALEKALEKYGKDVEIYQDPDVLDTWFSSSLW 527 >At2g34150.1 68415.m04180 expressed protein Length = 700 Score = 27.5 bits (58), Expect = 8.2 Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 1/42 (2%) Frame = +2 Query: 521 DPLEPWFNYIQWVEQSFPKHGHEXHIDKLIKXCL-QLFENDN 643 +PL P +QW+ P G E + + +K L Q FE +N Sbjct: 549 EPLPPPLPPLQWMVSKIPSAGFEDNNKQSLKDALTQAFEKNN 590 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,802,076 Number of Sequences: 28952 Number of extensions: 207186 Number of successful extensions: 402 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 398 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 402 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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