Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_A18
(649 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 46 4e-06
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 44 2e-05
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 40 2e-04
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 34 0.015
SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ... 29 0.76
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 0.76
SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi... 27 3.1
SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 26 4.1
SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizo... 25 7.1
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 25 9.4
SPAC637.06 |||alpha-1,2-galactosyltransferase |Schizosaccharomyc... 25 9.4
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 46.4 bits (105), Expect = 4e-06
Identities = 31/113 (27%), Positives = 43/113 (38%), Gaps = 3/113 (2%)
Frame = +2
Query: 284 SSHVEMMPKKLRLSL---CVGCGGQIHDQYILRVAPDLEWHAACLKCQECRQFLDESCTC 454
SSH P R + C CGG + I+ A + H C KC C Q L E
Sbjct: 240 SSHTSTKPVLYRGNSEKSCHSCGGSLRAGRIIS-ASGKKLHPQCFKCDTCSQNL-EHVGF 297
Query: 455 FVXDGKTYCKXXYTTLFGTKCDKCGAXFSKNDFVMRAKTNIYHIDCFXCCACA 613
+ +GK YC Y F +C C D + + +H + C C+
Sbjct: 298 YYREGKFYCHLDYHEQFSPRCKHCKTPI--EDQAVHINNDWFHENHHFCAGCS 348
Score = 39.9 bits (89), Expect = 3e-04
Identities = 27/103 (26%), Positives = 39/103 (37%)
Frame = +2
Query: 329 CVGCGGQIHDQYILRVAPDLEWHAACLKCQECRQFLDESCTCFVXDGKTYCKXXYTTLFG 508
C C I DQ + + D +H C C + + + C D +C+ Y +
Sbjct: 318 CKHCKTPIEDQAV-HINNDW-FHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYA 375
Query: 509 TKCDKCGAXFSKNDFVMRAKTNIYHIDCFXCCACARQLIPGDE 637
KC KC ++ YH C+ C AC L GDE
Sbjct: 376 VKCKKCRKPIL--GISVKGSDGEYHSQCWTCGACNALL--GDE 414
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 44.0 bits (99), Expect = 2e-05
Identities = 31/127 (24%), Positives = 49/127 (38%), Gaps = 6/127 (4%)
Frame = +2
Query: 278 DTSSHVEMMPKKLRLSLCVGCGGQIHDQYILRVAPDLEWHAACLKCQECRQFLDESCTCF 457
DTS + + K +C CG I QY+ R ++ +H C +C +C + + F
Sbjct: 99 DTSVFPKAVRKVSSSKICASCGQVISGQYV-RALGNI-YHLECFRCHDCNSLV--ASKFF 154
Query: 458 VXDGKT------YCKXXYTTLFGTKCDKCGAXFSKNDFVMRAKTNIYHIDCFXCCACARQ 619
D T C+ Y C CG + + + A +HI+ F C C
Sbjct: 155 PIDDPTLNKQVPLCETDYFRRLDLLCASCG--MALRGYYITALNKKFHIEHFTCSLCYTV 212
Query: 620 LIPGDEF 640
P D +
Sbjct: 213 FGPNDSY 219
Score = 43.2 bits (97), Expect = 3e-05
Identities = 22/91 (24%), Positives = 37/91 (40%), Gaps = 2/91 (2%)
Frame = +2
Query: 326 LCVGCGGQIHDQYILRVAPDLEWHAACLKCQECRQFLDESCTCFVXDGKTYCKXXYTTLF 505
LC CG + YI A + ++H C C + + + +GK YC Y+TLF
Sbjct: 179 LCASCGMALRGYYI--TALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLF 236
Query: 506 GTKCDKCGAXFSKN--DFVMRAKTNIYHIDC 592
+C C + + + +H+ C
Sbjct: 237 AARCCGCDGPILRQFVEVYRNGVSQNWHVPC 267
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 40.3 bits (90), Expect = 2e-04
Identities = 23/80 (28%), Positives = 34/80 (42%), Gaps = 2/80 (2%)
Frame = +2
Query: 392 WHAACLKCQECRQFLDESCTCFVX--DGKTYCKXXYTTLFGTKCDKCGAXFSKNDFVMRA 565
WH C C +C + L+ S V DG+ C T C C D+ + +
Sbjct: 40 WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSCAHT-----CTACRMRIK--DYALMS 92
Query: 566 KTNIYHIDCFXCCACARQLI 625
+ YH +CF C C +Q+I
Sbjct: 93 GYDSYHRECFRCHDCRKQII 112
Score = 34.3 bits (75), Expect = 0.015
Identities = 13/47 (27%), Positives = 24/47 (51%)
Frame = +2
Query: 509 TKCDKCGAXFSKNDFVMRAKTNIYHIDCFXCCACARQLIPGDEFALR 649
T C +CG F + + + +++H DCF C C + L D+ ++
Sbjct: 17 TVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQ 63
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 34.3 bits (75), Expect = 0.015
Identities = 21/72 (29%), Positives = 30/72 (41%)
Frame = +2
Query: 392 WHAACLKCQECRQFLDESCTCFVXDGKTYCKXXYTTLFGTKCDKCGAXFSKNDFVMRAKT 571
WH+ C KC C + LD S F D + K + L C+ C + + V ++
Sbjct: 44 WHSDCFKCVNCNKKLDPSSEDFSQDDQ---KQIFCKLCVDICNGCSTPICEFNAVSNSQA 100
Query: 572 NIYHIDCFXCCA 607
N H C C A
Sbjct: 101 N--HPCCSNCRA 110
Score = 27.1 bits (57), Expect = 2.3
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +2
Query: 578 YHIDCFXCCACARQLIPGDE 637
+H DCF C C ++L P E
Sbjct: 44 WHSDCFKCVNCNKKLDPSSE 63
>SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 345
Score = 28.7 bits (61), Expect = 0.76
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -2
Query: 231 PPPPPLAITITDDHTEPTFE 172
PPPPP+A+ T+D + P E
Sbjct: 321 PPPPPVAVNSTNDESAPQNE 340
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 28.7 bits (61), Expect = 0.76
Identities = 17/49 (34%), Positives = 30/49 (61%)
Frame = +3
Query: 312 SSGSRCASAAEVRSTTSTSSGWPLTWSGTRPA*SVRNAGSSSTNPARAS 458
SS S +S++ S++S+SS P+T S + S ++ SSS++ +R S
Sbjct: 187 SSSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSRPS 235
Score = 25.0 bits (52), Expect = 9.4
Identities = 19/67 (28%), Positives = 36/67 (53%)
Frame = +3
Query: 258 CTITISWTLHLTLK*CQKSSGSRCASAAEVRSTTSTSSGWPLTWSGTRPA*SVRNAGSSS 437
C + W+++LT +++ S++ V STTS+SS + S T S ++ SSS
Sbjct: 107 CGGDLYWSVYLTGNGVLQTT----VSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSS 162
Query: 438 TNPARAS 458
++ + +S
Sbjct: 163 SSSSSSS 169
>SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein
Usp104|Schizosaccharomyces pombe|chr 1|||Manual
Length = 695
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/53 (26%), Positives = 24/53 (45%), Gaps = 4/53 (7%)
Frame = +1
Query: 169 DFERWFSVVV----SDGDGQRRWWRADTVPTRHDAPSQYHGHFISR*NDAKKA 315
DFE+ S + + DG++ W+ +T + D P +Y + KKA
Sbjct: 67 DFEKKLSKLAWKEYATADGKKYWYNVNTRESVWDIPDEYKAALVDEPEQQKKA 119
>SPCC553.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 349
Score = 26.2 bits (55), Expect = 4.1
Identities = 17/54 (31%), Positives = 31/54 (57%)
Frame = +3
Query: 312 SSGSRCASAAEVRSTTSTSSGWPLTWSGTRPA*SVRNAGSSSTNPARASLXTER 473
SS S +S++ V STTS+SS + + + + S ++ SSS + + +S + R
Sbjct: 118 SSSSSSSSSSLVSSTTSSSSSATPSTTSSSSSSSSSSSSSSSKSSSSSSKSSSR 171
>SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1513
Score = 25.4 bits (53), Expect = 7.1
Identities = 12/27 (44%), Positives = 15/27 (55%), Gaps = 1/27 (3%)
Frame = +2
Query: 389 EW-HAACLKCQECRQFLDESCTCFVXD 466
EW HA CLK + + DE TC + D
Sbjct: 1117 EWYHAKCLKMSKKKLRQDEKFTCPICD 1143
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/37 (35%), Positives = 15/37 (40%)
Frame = +2
Query: 506 GTKCDKCGAXFSKNDFVMRAKTNIYHIDCFXCCACAR 616
GT KCG F K + R KT + C C R
Sbjct: 90 GTCESKCGHIFRKGEVFYRCKTCSVDSNSALCVKCFR 126
>SPAC637.06 |||alpha-1,2-galactosyltransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 25.0 bits (52), Expect = 9.4
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = -3
Query: 377 PPGGCTGRGSDLRSRRTTRAGAFLAS 300
PPG C G G + R + G FL +
Sbjct: 288 PPGACAGEGENPRFFYNQKEGDFLVN 313
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,545,415
Number of Sequences: 5004
Number of extensions: 49073
Number of successful extensions: 151
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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