BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_A12 (525 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 234 7e-63 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 234 7e-63 SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 149 3e-37 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 149 3e-37 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 149 3e-37 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 89 5e-19 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 45 8e-06 SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 44 2e-05 SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 41 1e-04 SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 29 0.56 SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha... 25 5.2 SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha... 25 5.2 SPBC2F12.09c |atf21||transcription factor Atf21|Schizosaccharomy... 25 9.1 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 234 bits (572), Expect = 7e-63 Identities = 109/123 (88%), Positives = 114/123 (92%) Frame = +2 Query: 41 LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220 +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 221 IQKESTLHLVLRLXGGTIEPSLRILAMKYNCXKMICRKCYARLHPRATNCRKTKCGHTNN 400 IQKESTLHLVLRL GG IEPSL+ LA KYNC K ICRKCYARL PRATNCRK KCGHTN Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQ 120 Query: 401 LRP 409 LRP Sbjct: 121 LRP 123 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 234 bits (572), Expect = 7e-63 Identities = 109/123 (88%), Positives = 114/123 (92%) Frame = +2 Query: 41 LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220 +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 221 IQKESTLHLVLRLXGGTIEPSLRILAMKYNCXKMICRKCYARLHPRATNCRKTKCGHTNN 400 IQKESTLHLVLRL GG IEPSL+ LA KYNC K ICRKCYARL PRATNCRK KCGHTN Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQ 120 Query: 401 LRP 409 LRP Sbjct: 121 LRP 123 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 149 bits (360), Expect = 3e-37 Identities = 71/76 (93%), Positives = 75/76 (98%) Frame = +2 Query: 41 LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220 +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 221 IQKESTLHLVLRLXGG 268 IQKESTLHLVLRL GG Sbjct: 61 IQKESTLHLVLRLRGG 76 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 149 bits (360), Expect = 3e-37 Identities = 71/76 (93%), Positives = 75/76 (98%) Frame = +2 Query: 41 LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220 +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 221 IQKESTLHLVLRLXGG 268 IQKESTLHLVLRL GG Sbjct: 61 IQKESTLHLVLRLRGG 76 Score = 149 bits (360), Expect = 3e-37 Identities = 71/76 (93%), Positives = 75/76 (98%) Frame = +2 Query: 41 LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220 +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 77 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 136 Query: 221 IQKESTLHLVLRLXGG 268 IQKESTLHLVLRL GG Sbjct: 137 IQKESTLHLVLRLRGG 152 Score = 149 bits (360), Expect = 3e-37 Identities = 71/76 (93%), Positives = 75/76 (98%) Frame = +2 Query: 41 LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220 +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 212 Query: 221 IQKESTLHLVLRLXGG 268 IQKESTLHLVLRL GG Sbjct: 213 IQKESTLHLVLRLRGG 228 Score = 149 bits (360), Expect = 3e-37 Identities = 71/76 (93%), Positives = 75/76 (98%) Frame = +2 Query: 41 LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220 +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 288 Query: 221 IQKESTLHLVLRLXGG 268 IQKESTLHLVLRL GG Sbjct: 289 IQKESTLHLVLRLRGG 304 Score = 149 bits (360), Expect = 3e-37 Identities = 71/76 (93%), Positives = 75/76 (98%) Frame = +2 Query: 41 LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220 +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 364 Query: 221 IQKESTLHLVLRLXGG 268 IQKESTLHLVLRL GG Sbjct: 365 IQKESTLHLVLRLRGG 380 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 149 bits (360), Expect = 3e-37 Identities = 71/76 (93%), Positives = 75/76 (98%) Frame = +2 Query: 41 LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220 +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 221 IQKESTLHLVLRLXGG 268 IQKESTLHLVLRL GG Sbjct: 61 IQKESTLHLVLRLRGG 76 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 88.6 bits (210), Expect = 5e-19 Identities = 38/75 (50%), Positives = 55/75 (73%) Frame = +2 Query: 47 IFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 226 I VKTLTGK I L+++ +D + +K ++++KEGIPP QQRLI+AGKQ+ D + Y+++ Sbjct: 3 IKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLE 62 Query: 227 KESTLHLVLRLXGGT 271 S LHLVL L GG+ Sbjct: 63 GGSVLHLVLALRGGS 77 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 44.8 bits (101), Expect = 8e-06 Identities = 22/75 (29%), Positives = 40/75 (53%) Frame = +2 Query: 35 TQLQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSD 214 T + + +K + + V++ ++ +K I I ++QRLI+AG+ L+D +L Sbjct: 2 TNISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKT 61 Query: 215 YNIQKESTLHLVLRL 259 Y IQ ++HLV L Sbjct: 62 YKIQDGHSIHLVKTL 76 >SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 43.6 bits (98), Expect = 2e-05 Identities = 18/54 (33%), Positives = 35/54 (64%) Frame = +2 Query: 71 KTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE 232 K + +V A I +K KIQ ++ ++Q+LI++G+ L D +T+ +YNI+++ Sbjct: 12 KFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQ 65 >SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 40.7 bits (91), Expect = 1e-04 Identities = 19/58 (32%), Positives = 33/58 (56%) Frame = +2 Query: 68 GKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL 241 GK LE+E ++T +K ++ +PP++Q++I G QL+D L I+ +TL Sbjct: 10 GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67 >SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces pombe|chr 2|||Manual Length = 603 Score = 28.7 bits (61), Expect = 0.56 Identities = 21/81 (25%), Positives = 38/81 (46%), Gaps = 2/81 (2%) Frame = +2 Query: 35 TQLQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSD 214 ++ +I V T+ K +V + T+ +K I P D+ +LI AG+ L + L + Sbjct: 2 SEYRIRVTTVDQKVGIFQVPRTKTVLELKELIAVTFEAPADRLKLIHAGRVLRNETPLEE 61 Query: 215 --YNIQKESTLHLVLRLXGGT 271 ++ T HLV+ + T Sbjct: 62 ILHDATDLVTFHLVIAVFNST 82 >SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccharomyces pombe|chr 2|||Manual Length = 485 Score = 25.4 bits (53), Expect = 5.2 Identities = 27/116 (23%), Positives = 48/116 (41%), Gaps = 13/116 (11%) Frame = +2 Query: 56 KTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA------GKQLE-------D 196 K TGKT + + + ++ K+KIQ +P + L + GK + Sbjct: 89 KNGTGKTAAFVIPSLEKVDTKKSKIQTLILVPTRELALQTSQVCKTLGKHMNVKVMVTTG 148 Query: 197 GRTLSDYNIQKESTLHLVLRLXGGTIEPSLRILAMKYNCXKMICRKCYARLHPRAT 364 G TL D I+ T+H+V+ G ++ + + +A C + + L P T Sbjct: 149 GTTLRDDIIRLNDTVHIVVGTPGRVLDLAGKGVADFSECTTFVMDEADKLLSPEFT 204 >SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 541 Score = 25.4 bits (53), Expect = 5.2 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = -3 Query: 115 IFDSVGSFHLQCNGLPRKGFDEYLQ 41 IF VG+F C+ L ++GF E L+ Sbjct: 43 IFPGVGNFGFVCDSLAKQGFLEPLR 67 >SPBC2F12.09c |atf21||transcription factor Atf21|Schizosaccharomyces pombe|chr 2|||Manual Length = 355 Score = 24.6 bits (51), Expect = 9.1 Identities = 18/64 (28%), Positives = 25/64 (39%), Gaps = 3/64 (4%) Frame = +2 Query: 62 LTGKTITLEVEASDTIENVKAKIQDKEGIP---PDQQRLIFAGKQLEDGRTLSDYNIQKE 232 L G T EV T+ + + +G P P + I A +G TL+ YN Sbjct: 94 LKGPERTSEVSLPQTVNLSEISNNNDKGQPTNTPPVRSTIVAPSLYSEGSTLNHYNNGSH 153 Query: 233 STLH 244 LH Sbjct: 154 QVLH 157 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,091,950 Number of Sequences: 5004 Number of extensions: 42146 Number of successful extensions: 115 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 109 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 115 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 214353836 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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