BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP27_F_C05
(1189 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 56 1e-08
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 39 0.001
SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha... 30 0.72
SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|c... 29 1.7
SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy... 27 3.9
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 26 8.9
SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyc... 26 8.9
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 55.6 bits (128), Expect = 1e-08
Identities = 34/102 (33%), Positives = 49/102 (48%), Gaps = 4/102 (3%)
Frame = +1
Query: 244 PSASRAARG---GLAVDXXSIPHRPPFIAHISNLPYXVEXSAIAXLFAD-LKVTNLSLPX 411
P SR G G D IP PPF AH+ NL + + + + F + + L +
Sbjct: 68 PPESRREGGMGSGYQRDAIPIPSEPPFTAHVGNLSFDLTENDLGDFFGEGVTSIRLVIDP 127
Query: 412 XGDRLKGHGYVDFEXRENLIEALNMPDLTIGGRRVRIEVSTP 537
+R +G GYV+FE + L AL + + GR VRI V+ P
Sbjct: 128 LTERSRGFGYVEFETADTLSAALALSGEDLMGRPVRITVAEP 169
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 39.1 bits (87), Expect = 0.001
Identities = 31/107 (28%), Positives = 45/107 (42%)
Frame = +1
Query: 217 SHPRAPVVLPSASRAARGGLAVDXXSIPHRPPFIAHISNLPYXVEXSAIAXLFADLKVTN 396
S+PR P A + R G D S P F+ ++S + S D++
Sbjct: 339 SNPRPANPQPYAQQ--RAGNFGDQLSEPSDTVFVGNLSFNATEDDLSTAFGGCGDIQSIR 396
Query: 397 LSLPXXGDRLKGHGYVDFEXRENLIEALNMPDLTIGGRRVRIEVSTP 537
L RLKG GYV F ++ + + M I GR R++ STP
Sbjct: 397 LPTDPQSGRLKGFGYVTFSDIDSAKKCVEMNGHFIAGRPCRLDFSTP 443
Score = 29.9 bits (64), Expect = 0.72
Identities = 17/40 (42%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = +1
Query: 421 RLKGHGYVDFEXRENLIEALNMPDL-TIGGRRVRIEVSTP 537
R KG+GYVDFE E A+ I GR V +++S P
Sbjct: 302 RSKGYGYVDFETPEAAKAAVAANGTKEIDGRMVNLDLSNP 341
>SPBC660.15 |||mRNA cleavage factor complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 474
Score = 29.9 bits (64), Expect = 0.72
Identities = 15/40 (37%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
Frame = +1
Query: 421 RLKGHGYVDFEXRENLIEA-LNMPDLTIGGRRVRIEVSTP 537
R +G G+V +E E+ +EA ++ P +TI G+ V ++ +TP
Sbjct: 286 RPRGFGFVTYE-NESAVEATMSQPYITIHGKPVEVKRATP 324
>SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 489
Score = 28.7 bits (61), Expect = 1.7
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -2
Query: 567 ASTSSHSTVIWSRHLNSY 514
AS +S ST+ W HLNSY
Sbjct: 250 ASFASQSTIAWQSHLNSY 267
>SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 436
Score = 27.5 bits (58), Expect = 3.9
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +2
Query: 548 EWDEVDALIEIGTMTLSVQWVTGAPDPALYPTRPAA 655
++ ++DA I++ LS+ W P P L P +A
Sbjct: 356 QFSDIDAWIQVACPRLSIDWGYAFPAPLLTPYEASA 391
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 26.2 bits (55), Expect = 8.9
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 527 CLLQMTVEWDEVDALIEIGTMTLSVQWVTGAPDP 628
CL + +W ++I T+ LS+Q + GAP+P
Sbjct: 86 CLSTLKKDWSPA---LQIRTVLLSIQALMGAPNP 116
>SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 434
Score = 26.2 bits (55), Expect = 8.9
Identities = 15/35 (42%), Positives = 17/35 (48%)
Frame = +1
Query: 421 RLKGHGYVDFEXRENLIEALNMPDLTIGGRRVRIE 525
R KG YVDF E + ALN L I + IE
Sbjct: 348 RRKGTAYVDFVNHECVQLALNKKTLQINNATLNIE 382
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,886,246
Number of Sequences: 5004
Number of extensions: 40828
Number of successful extensions: 102
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 101
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 639490422
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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