Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP26_F_H02
(1186 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 483 e-137
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 479 e-136
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 247 2e-66
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 184 2e-47
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.044
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 483 bits (1192), Expect = e-137
Identities = 217/311 (69%), Positives = 255/311 (81%)
Frame = +2
Query: 137 MRECISVHVGQAGVQXGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 316
MRE IS+HVGQAG Q GNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 317 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 496
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 497 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 676
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 677 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 856
VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITA
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240
Query: 857 SLRFDGALNVDLTEFQTNLVPYPRIHXPLVTYAPVISAXKXYHEQLSVAXITXAXFXPXN 1036
SLRF+G+LNVDL EFQTNLVPYPRIH PLVTYAP++SA K +HE SV IT F P N
Sbjct: 241 SLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYN 300
Query: 1037 XMVKCXPRHGK 1069
MVKC PR G+
Sbjct: 301 QMVKCDPRAGR 311
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 479 bits (1181), Expect = e-136
Identities = 220/316 (69%), Positives = 257/316 (81%), Gaps = 5/316 (1%)
Frame = +2
Query: 137 MRECISVHVGQAGVQXGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 301
MRE ISVHVGQAGVQ GNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 302 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 481
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 482 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 661
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 662 QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIV 841
QVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+V
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239
Query: 842 SSITASLRFDGALNVDLTEFQTNLVPYPRIHXPLVTYAPVISAXKXYHEQLSVAXITXAX 1021
SSITASLRF G+LNVDL EFQTNLVPYPRIH PLVTY+P++SA K +HE SV IT
Sbjct: 240 SSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQC 299
Query: 1022 FXPXNXMVKCXPRHGK 1069
F P N MVKC PR G+
Sbjct: 300 FEPYNQMVKCDPRTGR 315
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 247 bits (605), Expect = 2e-66
Identities = 120/311 (38%), Positives = 175/311 (56%)
Frame = +2
Query: 137 MRECISVHVGQAGVQXGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 316
MRE + + GQ G Q G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 317 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 496
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 497 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 676
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 677 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 856
VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTT 238
Query: 857 SLRFDGALNVDLTEFQTNLVPYPRIHXPLVTYAPVISAXKXYHEQLSVAXITXAXFXPXN 1036
S RF G LN DL + N+VP+PR+H +V +AP+ + + +SV +T F N
Sbjct: 239 SFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANN 298
Query: 1037 XMVKCXPRHGK 1069
MV PRHG+
Sbjct: 299 MMVAADPRHGR 309
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 184 bits (449), Expect = 2e-47
Identities = 103/312 (33%), Positives = 164/312 (52%), Gaps = 6/312 (1%)
Frame = +2
Query: 140 RECISVHVGQAGVQXGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 319
RE I++ GQ G Q G+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 320 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVL 493
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 494 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 670
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 671 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSI 850
VV+PYNS+L ++D ++DN A+ I L + PT+ N+L+ ++S+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239
Query: 851 TASLRFDGALNVDLTEFQTNLVPYPRIHXPLVTYAPVISAXKXYHEQL---SVAXITXAX 1021
T +LR+ G +N DL +L+P PR H L +Y P + + + +V +
Sbjct: 240 TTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRL 299
Query: 1022 FXPXNXMVKCXP 1057
P N MV P
Sbjct: 300 LLPKNQMVSVNP 311
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.9 bits (74), Expect = 0.044
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = -1
Query: 919 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 758
R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,048,317
Number of Sequences: 5004
Number of extensions: 80847
Number of successful extensions: 245
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 235
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 240
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 637498240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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