Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP26_F_E09
         (1186 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF...   116   8e-27
SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ...   107   3e-24
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa...    29   1.7  
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    28   2.2  

>SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E
           4F complex subunit |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 243

 Score =  116 bits (278), Expect = 8e-27
 Identities = 65/185 (35%), Positives = 94/185 (50%), Gaps = 4/185 (2%)
 Frame = +3

Query: 156 APCXFXSSTRLQNQWSLWFYANX-RNKTWXEHLIELTXFDTVEAFWXLYHHIKLPSELRQ 332
           A   F  +  LQ++W+LWF     +   W + L E+  F TVE FW ++  I   S L  
Sbjct: 56  AETAFVKTHPLQHEWTLWFLKPPTQGLEWSDLLKEIISFKTVEEFWGIFKTISKASMLPA 115

Query: 333 GHDYAVFKQGIRPMWEXDANKXGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFE-NSD 509
             DY+ F +GIRP WE   N  GG+W  + + K + ++LD  WL +VL  IGE  +    
Sbjct: 116 KSDYSYFLKGIRPEWEDPQNMNGGKW--AYQSKHKGSNLDELWLYMVLAAIGETLDPTGK 173

Query: 510 EICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGK--IGFQVHRDTMVKH 683
           E+ G V N+R    +IA+WT +   +    +IG + KE LGI  K  I +  H D+    
Sbjct: 174 EVTGVVCNMRKGFYRIAVWTRNCNDKDVLEKIGLRFKEVLGISDKETIEYSAHEDSSKAG 233

Query: 684 SSATK 698
           S   K
Sbjct: 234 SMRAK 238


>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
           complex subunit |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 218

 Score =  107 bits (257), Expect = 3e-24
 Identities = 58/180 (32%), Positives = 87/180 (48%), Gaps = 4/180 (2%)
 Frame = +3

Query: 186 LQNQWSLWFYANXR-NKTWXEHLIELTXFDTVEAFWXLYHHIKLPSELRQGHDYAVFKQG 362
           L   W+LWF         W E    +  F++VE FW ++++I   S L    DY+ F++G
Sbjct: 40  LARPWTLWFLMPPTPGLEWNELQKNIITFNSVEEFWGIHNNINPASSLPIKSDYSFFREG 99

Query: 363 IRPMWEXDANKXGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFE-NSDEICGAVVNVR 539
           +RP WE   NK GG+W     K +    LD  WL  VL  IGE  +    E+ G V+N+R
Sbjct: 100 VRPEWEDVHNKTGGKWAFQ-NKGRGGNALDEMWLTTVLAAIGETLDPTGQEVMGVVINMR 158

Query: 540 PKVDKIAIWTADAMKQHATIEIGKKLKEQLGI--HGKIGFQVHRDTMVKHSSATKNLYTV 713
               ++A+WT     +   +EIG + K+ L +     I F  H D+    S+  K   +V
Sbjct: 159 KGFYRLAVWTKSCNNREVLMEIGTRFKQVLNLPRSETIEFSAHEDSSKSGSTRAKTRMSV 218


>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 28.7 bits (61), Expect = 1.7
 Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
 Frame = +2

Query: 632 DTRQDW-FPSTQRH-NG*A*FCDQESIHCLVLAL 727
           D  Q+W FP TQ++ N    FC+Q   H L+LAL
Sbjct: 98  DAMQNWIFPQTQQYRNYQKEFCEQALFHNLLLAL 131


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
            E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 28.3 bits (60), Expect = 2.2
 Identities = 21/82 (25%), Positives = 35/82 (42%), Gaps = 1/82 (1%)
 Frame = -1

Query: 1042 PTSRVAYLTTYNYVTLHTDTRA*V*YKIRLLTFYSSRGLYHVTM*DKYDYKN*TNTTSVK 863
            P ++  +   Y Y+ L  D      +K  +  FY      H  M  + +Y+N T   S+ 
Sbjct: 1093 PRNKTIFRILYEYIQLSDDHTLDDFWKTPVPIFYGEPDCIHNDMKGELNYENETEGFSIN 1152

Query: 862  YNFIL-LL*LRKFSLWDVLNIF 800
               IL LL +  + + DV  +F
Sbjct: 1153 IREILDLLSILYYGIRDVHTLF 1174


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,652,963
Number of Sequences: 5004
Number of extensions: 65382
Number of successful extensions: 153
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 637498240
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)