Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP25_F_P07
(1178 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC27E2.02 |||IMPACT homolog|Schizosaccharomyces pombe|chr 1|||... 89 1e-18
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 39 0.002
SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 29 1.7
SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosacc... 28 2.2
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 2.2
SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 27 3.8
SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces ... 27 3.8
SPAC2G11.04 |||RNA-binding protein, G-patch type |Schizosaccharo... 27 5.1
SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyc... 27 5.1
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 27 5.1
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 26 8.8
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 26 8.8
SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6 |Schi... 26 8.8
>SPAC27E2.02 |||IMPACT homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 280
Score = 89.0 bits (211), Expect = 1e-18
Identities = 79/237 (33%), Positives = 119/237 (50%), Gaps = 20/237 (8%)
Frame = +3
Query: 225 VDNLSRQAEEIEALKSIYTEELTIDSEITRSYTIRIEENKKEVLLYVTLPEDYPSISPPK 404
++N +E+ AL+SIY L SE + +YT+ I ++ V L + P DYP+ +P
Sbjct: 1 MENNEEFQDELLALESIYPSCLLPISEQSFTYTLSIPDSS--VRLNIQFPLDYPNSAPTV 58
Query: 405 FELSAPWMDRQTKTNLHKTLHEIYLDNV--GETVIFQWVEMIREVL-----QTVCKVEKK 563
L A +D KTL E L +V G+ IF ++++++E++ Q + E K
Sbjct: 59 --LDAYGID--------KTLAEDVLLSVATGDVCIFSYMDLLKELVDIDAEQAAAERESK 108
Query: 564 I-EVTEPEVD-SLDLTTIEINCPEIT-----------HGEIIADRKSIFQGHAAEVHSID 704
+ E ++ E L+ + PEI E I DRKS F HA V+S +
Sbjct: 109 LQEESDKETPVMLNKSHYVAKTPEIQDEPWKPKFDWKESEPITDRKSTFMAHATRVYSTE 168
Query: 705 DVKAVLNKLKQNRKILNATHNMYAYRIXRKTAKGTNVLQDCDDDGEAHAGGXMLXLL 875
+V+ L L ++K+ A HNM AYRI + NV+QD DDDGE+ AG M LL
Sbjct: 169 EVREALEDLYMDKKVAKANHNMVAYRI---ISPNGNVIQDNDDDGESAAGSRMSHLL 222
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 38.7 bits (86), Expect = 0.002
Identities = 18/62 (29%), Positives = 36/62 (58%)
Frame = +3
Query: 348 EVLLYVTLPEDYPSISPPKFELSAPWMDRQTKTNLHKTLHEIYLDNVGETVIFQWVEMIR 527
++++ LPE YP SPP F L + W+ + K L +L +++ + + + V+F +E +R
Sbjct: 71 DLVMEFFLPEAYPFNSPPTFFLKSSWLPLKQKRVLTSSLIKLW-NEIHDCVLFDAIEHVR 129
Query: 528 EV 533
+
Sbjct: 130 SI 131
>SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1647
Score = 28.7 bits (61), Expect = 1.7
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = -2
Query: 649 MISPCVISGQFISIVVRSKLSTSGSVTSIFFSTLHTVCSTSLIISTH 509
+I+P +++ QF ++ + L T+GS + L+ + S ISTH
Sbjct: 1437 LITPNLLTSQFFDVLTKDALYTNGSCWELSVIILNFLHHVSPDISTH 1483
>SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase
Ubp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1141
Score = 28.3 bits (60), Expect = 2.2
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = -3
Query: 465 ARFCEDSFWFVCPSTVQTIRISVEKSTGNLLVMSRKE 355
A F E+ FW VC T + + + V + G L+ +E
Sbjct: 53 AVFDENDFWCVCQKTRKHLHVKVRRDRGKPLIEEPEE 89
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 28.3 bits (60), Expect = 2.2
Identities = 22/81 (27%), Positives = 36/81 (44%)
Frame = -2
Query: 721 STAFTSSILCTSAACPWNMLFLSAMISPCVISGQFISIVVRSKLSTSGSVTSIFFSTLHT 542
ST T+S TS++ + S++ S S S + S S S+TS ++ T
Sbjct: 95 STNSTTSASPTSSSLTSSSATSSSLASSSTTSSSLASSSITSSSLASSSITSSSLASSST 154
Query: 541 VCSTSLIISTH*NITVSPTLS 479
S+ ST+ + +PT S
Sbjct: 155 TSSSLASSSTNSTTSATPTSS 175
>SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 188
Score = 27.5 bits (58), Expect = 3.8
Identities = 10/32 (31%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -3
Query: 858 YLRRELP-RHHHSLEVHSYL*QFFFXFYMHTY 766
+ R +LP HHH+ + ++ FFF F+ ++
Sbjct: 56 FRRLKLPYHHHHTFTIEAFFYWFFFFFFFFSH 87
>SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 878
Score = 27.5 bits (58), Expect = 3.8
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +3
Query: 702 DDVKAVLNKLKQNRKILNATHNMYAYRIXRKTAKGTNVLQDCDDD 836
++V AV++ +K N + + T N+Y R+ R G VL C++D
Sbjct: 787 ENVPAVISIVKSNGGVCS-TLNVYNKRLARHLEDGNVVLITCNED 830
>SPAC2G11.04 |||RNA-binding protein, G-patch type
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 301
Score = 27.1 bits (57), Expect = 5.1
Identities = 19/83 (22%), Positives = 36/83 (43%), Gaps = 5/83 (6%)
Frame = +3
Query: 330 IEENKKEVLLYVTLPEDYPSI----SPPKFELSAP-WMDRQTKTNLHKTLHEIYLDNVGE 494
I++N E + +V D S +P F++ + + N ++ DN+G+
Sbjct: 87 IQKNANENIPHVQCRNDSQSTVTTRNPTPFKIEVGIYPSKPNNLNKEPPASTVHSDNLGD 146
Query: 495 TVIFQWVEMIREVLQTVCKVEKK 563
V +WVE+ + T V K+
Sbjct: 147 AVEHEWVELYDPLFPTSYSVFKE 169
>SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 413
Score = 27.1 bits (57), Expect = 5.1
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -1
Query: 839 PVIITVLKYIRTFSSFSXYSIC 774
P I V K RTF+SFS +++C
Sbjct: 128 PPCIRVWKMARTFNSFSPHTVC 149
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 27.1 bits (57), Expect = 5.1
Identities = 32/112 (28%), Positives = 52/112 (46%), Gaps = 14/112 (12%)
Frame = +3
Query: 243 QAEEIEALKSIYT---EELTIDS--EITRS--YTIRI---EENKKEVL---LYVTLPEDY 383
Q EIEALK+I+ EEL + + +T Y I + N K + L + L Y
Sbjct: 14 QENEIEALKAIFMDDFEELKVRNAWNVTNGHVYCIHLCSRSANSKSIAKLDLCIELGRSY 73
Query: 384 PSISPP-KFELSAPWMDRQTKTNLHKTLHEIYLDNVGETVIFQWVEMIREVL 536
P + P K + ++ Q + L K L D +GE +IF+ ++++ L
Sbjct: 74 PYVKPVIKLQNGENVLNSQIRFLLDK-LDTKAKDLLGEEMIFELASIVQDYL 124
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 26.2 bits (55), Expect = 8.8
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +3
Query: 246 AEEIEALKSIYTEELTIDSEITRSYTIRIEENKKEVLLYVTLPEDYPSISP 398
A ++EAL ++ ++L +DS + R+ E V+L+ T+ + PS P
Sbjct: 1272 AHQVEALMELFEQKLNVDS-LPTDANDRLRE--ATVVLFGTVAQHLPSNDP 1319
>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 26.2 bits (55), Expect = 8.8
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -2
Query: 625 GQFISIVVRSKLSTSGSVTSIFFSTLHTV 539
G +I ++ L T G +TS FFS L +V
Sbjct: 105 GLLQNINTKNPLGTGGKITSAFFSLLQSV 133
>SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 624
Score = 26.2 bits (55), Expect = 8.8
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +1
Query: 424 GWTDKPKRIFTKPCTKYT 477
GW P+ +FT+P T++T
Sbjct: 430 GWLSLPRSLFTQPFTEFT 447
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,245,953
Number of Sequences: 5004
Number of extensions: 60828
Number of successful extensions: 235
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 222
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 633513876
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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