Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP25_F_J14
         (1212 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   392   e-110
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   391   e-110
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   195   1e-50
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   159   7e-40

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
            2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  392 bits (966), Expect = e-110
 Identities = 189/315 (60%), Positives = 224/315 (71%), Gaps = 4/315 (1%)
 Frame = +1

Query: 136  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 315
            MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1    MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 316  HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 495
            +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61   YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 496  RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 675
            +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121  KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 676  VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 855
            VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITA
Sbjct: 181  VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240

Query: 856  SLRSTAL*MWTSPSSXLTWCLTPYP---XPTGHVRASXXXXKAYMNXXPXRN-XXACFXP 1023
            SLR           +     L PYP    P           KA+      +     CF P
Sbjct: 241  SLRFEG--SLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEP 298

Query: 1024 XNXMXKXXPRXGXXM 1068
             N M K  PR G  M
Sbjct: 299  YNQMVKCDPRAGRYM 313


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 455

 Score =  391 bits (963), Expect = e-110
 Identities = 193/320 (60%), Positives = 227/320 (70%), Gaps = 9/320 (2%)
 Frame = +1

Query: 136  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 300
            MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1    MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 301  TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 480
            TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60   TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 481  DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 660
            D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120  DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 661  QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIV 840
            QVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+V
Sbjct: 180  QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239

Query: 841  SSITASLRSTAL*MWTSPSSXLTWCLTPYP---XPTGHVRASXXXXKAYMNXXPXRN-XX 1008
            SSITASLR           +     L PYP    P           KA+      +    
Sbjct: 240  SSITASLRFAG--SLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITN 297

Query: 1009 ACFXPXNXMXKXXPRXGXXM 1068
             CF P N M K  PR G  M
Sbjct: 298  QCFEPYNQMVKCDPRTGRYM 317


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  195 bits (475), Expect = 1e-50
 Identities = 94/243 (38%), Positives = 138/243 (56%)
 Frame = +1

Query: 136 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 315
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 316 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 495
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 496 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 675
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+ S  
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 676 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 855
           VVEPYN+ L+ H  +E+SD  F +DNEA+  I    L I+ P+Y +LN L+  +++ +T 
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTT 238

Query: 856 SLR 864
           S R
Sbjct: 239 SFR 241



 Score = 26.6 bits (56), Expect = 6.9
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = +3

Query: 858 FEIDGALNVDLTEFXTNLVPYP 923
           F   G LN DL +   N+VP+P
Sbjct: 240 FRFPGELNSDLRKLAVNMVPFP 261


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  159 bits (386), Expect = 7e-40
 Identities = 86/245 (35%), Positives = 137/245 (55%), Gaps = 3/245 (1%)
 Frame = +1

Query: 139 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 318
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 319 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVL 492
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 493 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 669
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 670 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSI 849
             VV+PYNS+L       ++D   ++DN A+  I    L  + PT+   N+L+  ++S+ 
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239

Query: 850 TASLR 864
           T +LR
Sbjct: 240 TTTLR 244


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,089,205
Number of Sequences: 5004
Number of extensions: 80970
Number of successful extensions: 238
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 655427878
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)