Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP25_F_I03
         (1229 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   479   e-136
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   475   e-135
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   237   2e-63
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   188   1e-48
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom...    34   0.046
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch...    27   4.0  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
            2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  479 bits (1182), Expect = e-136
 Identities = 214/298 (71%), Positives = 251/298 (84%)
 Frame = +3

Query: 138  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 317
            MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1    MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 318  HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 497
            +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61   YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 498  RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 677
            +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121  KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 678  VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 857
            VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITA
Sbjct: 181  VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240

Query: 858  SLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAXITNACFXP 1031
            SLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE  SV  ITN CF P
Sbjct: 241  SLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEP 298



 Score = 42.7 bits (96), Expect = 1e-04
 Identities = 20/39 (51%), Positives = 21/39 (53%)
 Frame = +1

Query: 1030 PANXMVKXXPRHGKYMACCXLYRGXXXPXDXXXAXXXXK 1146
            P N MVK  PR G+YMA C LYRG   P D   A    K
Sbjct: 298  PYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIK 336


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 455

 Score =  475 bits (1171), Expect = e-135
 Identities = 217/303 (71%), Positives = 253/303 (83%), Gaps = 5/303 (1%)
 Frame = +3

Query: 138  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 302
            MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1    MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 303  TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 482
            TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60   TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 483  DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 662
            D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120  DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 663  QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIV 842
            QVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+V
Sbjct: 180  QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239

Query: 843  SSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAXITNAC 1022
            SSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE  SV  ITN C
Sbjct: 240  SSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQC 299

Query: 1023 FXP 1031
            F P
Sbjct: 300  FEP 302



 Score = 43.2 bits (97), Expect = 8e-05
 Identities = 20/40 (50%), Positives = 22/40 (55%)
 Frame = +1

Query: 1030 PANXMVKXXPRHGKYMACCXLYRGXXXPXDXXXAXXXXKT 1149
            P N MVK  PR G+YMA C LYRG   P D   A    K+
Sbjct: 302  PYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKS 341


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 448

 Score =  237 bits (581), Expect = 2e-63
 Identities = 114/296 (38%), Positives = 170/296 (57%)
 Frame = +3

Query: 138  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 317
            MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1    MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 318  HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 497
            +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59   YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 498  RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 677
             +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+ S  
Sbjct: 119  VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 678  VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 857
            VVEPYN+ L+ H  +E+SD  F +DNEA+  I    L I+ P+Y +LN L+  +++ +T 
Sbjct: 179  VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTT 238

Query: 858  SLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAXITNACF 1025
            S RF G LN DL +   N+VP+PR+HF +V +AP+ +   +  + +SV  +T   F
Sbjct: 239  SFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMF 294



 Score = 29.9 bits (64), Expect = 0.76
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = +1

Query: 1036 NXMVKXXPRHGKYMACCXLYRG 1101
            N MV   PRHG+Y+    L+RG
Sbjct: 298  NMMVAADPRHGRYLTVAALFRG 319


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  188 bits (458), Expect = 1e-48
 Identities = 99/276 (35%), Positives = 155/276 (56%), Gaps = 3/276 (1%)
 Frame = +3

Query: 141 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 320
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 321 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVL 494
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 495 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 671
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 672 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSI 851
             VV+PYNS+L       ++D   ++DN A+  I    L  + PT+   N+L+  ++S+ 
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239

Query: 852 TASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAP 959
           T +LR+ G +N DL     +L+P PR HF L +Y P
Sbjct: 240 TTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTP 275


>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
           Msp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 903

 Score = 33.9 bits (74), Expect = 0.046
 Identities = 18/54 (33%), Positives = 30/54 (55%)
 Frame = -1

Query: 920 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 759
           R  +  E  + H  ++ + Q+SSD  ++ +D T  VG+G AL   I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216


>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 642

 Score = 27.5 bits (58), Expect = 4.0
 Identities = 31/111 (27%), Positives = 46/111 (41%), Gaps = 8/111 (7%)
 Frame = -1

Query: 953 VRDQWEVDTGVRHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAA 774
           V D   VDT V      +       +A ES R++   D  S    Q  +      E T A
Sbjct: 454 VADDDAVDTSVTATDDFD-ASASSSNAYESYRNTYTDDMDSSSIYQTSLSNVKTEETTPA 512

Query: 773 DV---IDGFIV-----DHESTVRVLKGCVGGEDRVVGLDDGSGNLRRGVDG 645
           +    +DG ++     +HE    V+    G E  ++  DDGSG +R  +DG
Sbjct: 513 EPASKVDGVVLYDFTGEHEG---VITASEGQEFTLLEPDDGSGWVRVKIDG 560


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,494,917
Number of Sequences: 5004
Number of extensions: 92214
Number of successful extensions: 287
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 269
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 282
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 667380970
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)