Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP25_F_A16
(1191 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 447 e-126
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 445 e-126
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 226 4e-60
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 175 1e-44
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.045
SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces p... 27 6.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 447 bits (1102), Expect = e-126
Identities = 210/329 (63%), Positives = 249/329 (75%), Gaps = 4/329 (1%)
Frame = +1
Query: 139 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 318
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 319 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 498
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 499 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 678
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 679 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 858
VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITA
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240
Query: 859 SLRFDGALNVDLTEFXTNLVPYPVSTSHWSRTRQSSLPXRPTMNSFPSPXS----QTHAS 1026
SLRF+G+LNVDL EF TNLVPYP H+ + P +F S
Sbjct: 241 SLRFEGSLNVDLAEFQTNLVPYP--RIHFPLVTYA--PIVSAAKAFHESNSVQEITNQCF 296
Query: 1027 XPXNXMVKXDPGXGKYMACCMLYRGDSYP 1113
P N MVK DP G+YMA C+LYRGD P
Sbjct: 297 EPYNQMVKCDPRAGRYMATCLLYRGDVIP 325
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 445 bits (1097), Expect = e-126
Identities = 215/334 (64%), Positives = 251/334 (75%), Gaps = 9/334 (2%)
Frame = +1
Query: 139 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 303
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 304 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 483
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 484 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 663
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 664 QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIV 843
QVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+V
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239
Query: 844 SSITASLRFDGALNVDLTEFXTNLVPYPVSTSHWSRTRQSSLPXRPTMNSFPSPXS---- 1011
SSITASLRF G+LNVDL EF TNLVPYP H+ S P +F S
Sbjct: 240 SSITASLRFAGSLNVDLNEFQTNLVPYP--RIHFPLVTYS--PIVSAAKAFHESNSVQEI 295
Query: 1012 QTHASXPXNXMVKXDPGXGKYMACCMLYRGDSYP 1113
P N MVK DP G+YMA C+LYRGD P
Sbjct: 296 TNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIP 329
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 226 bits (553), Expect = 4e-60
Identities = 115/321 (35%), Positives = 168/321 (52%)
Frame = +1
Query: 139 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 318
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 319 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 498
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 499 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 678
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 679 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 858
VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTT 238
Query: 859 SLRFDGALNVDLTEFXTNLVPYPVSTSHWSRTRQSSLPXRPTMNSFPSPXSQTHASXPXN 1038
S RF G LN DL + N+VP+P + + + P N
Sbjct: 239 SFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANN 298
Query: 1039 XMVKXDPGXGKYMACCMLYRG 1101
MV DP G+Y+ L+RG
Sbjct: 299 MMVAADPRHGRYLTVAALFRG 319
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 175 bits (426), Expect = 1e-44
Identities = 93/265 (35%), Positives = 148/265 (55%), Gaps = 3/265 (1%)
Frame = +1
Query: 142 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 321
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 322 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVL 495
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 496 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 672
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 673 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSI 852
VV+PYNS+L ++D ++DN A+ I L + PT+ N+L+ ++S+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239
Query: 853 TASLRFDGALNVDLTEFXTNLVPYP 927
T +LR+ G +N DL +L+P P
Sbjct: 240 TTTLRYPGYMNNDLVSIIASLIPSP 264
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.9 bits (74), Expect = 0.045
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = -1
Query: 921 RHQVSXELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 760
R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 595
Score = 26.6 bits (56), Expect = 6.8
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +1
Query: 877 ALNVDLTEFXTNLVPYPVSTSHWSRTRQSSLPXRPTMNSF 996
ALN+D T+F + L + S SH + RQ++L N +
Sbjct: 430 ALNIDTTQFPSYLSYHETSVSH-NTYRQNNLEELKNQNDY 468
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,432,213
Number of Sequences: 5004
Number of extensions: 89462
Number of successful extensions: 286
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 266
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 275
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 641482604
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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