Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP24_F_L13
         (891 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC644.18c |bet3||TRAPP complex subunit Bet3 |Schizosaccharomyc...   146   3e-36
SPBC1718.05 |trs31||TRAPP complex subunit Trs31 |Schizosaccharom...    38   0.003
SPBC215.14c |vps20||vacuolar sorting protein Vps20|Schizosacchar...    32   0.13 
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1...    29   1.2  

>SPAC644.18c |bet3||TRAPP complex subunit Bet3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 183

 Score =  146 bits (355), Expect = 3e-36
 Identities = 77/175 (44%), Positives = 119/175 (68%), Gaps = 3/175 (1%)
 Frame = +1

Query: 190 ISTKCLGKLLXLDAKKVNSELLTLTYGALVSQMLKETENTEDVNKHLERIGYNMGVRLIE 369
           +S   +G+ +     KVN+EL  LTYG++V+Q+ K+  N E VN+ L+++GYN+G+RLIE
Sbjct: 1   MSKSKIGEDVYKKVDKVNAELFVLTYGSIVAQLCKDM-NYEKVNEELDKMGYNIGIRLIE 59

Query: 370 DFLARTTSTRCLEMRETADKIQQ-AFKLYLSMQPTVTSWSSAGDEFSLVWDHCPLSEWVE 546
           DFLA+T   RC + RET + + +  FK++L+  P ++S S  G+ F L  D  PL+E+VE
Sbjct: 60  DFLAKTEWPRCADFRETGETVAKVGFKVFLNFSPIISSVSDDGNTFVLTLDDNPLAEFVE 119

Query: 547 MPSN--NGLKYCALIPGAIRGALQMVQLDVQCWFVQDQLKGDPVTELRVKYLKEI 705
           +P++    L Y  ++ G IRGAL+M+Q+DV   F++D L+GD  TE+RV +LK I
Sbjct: 120 LPADARQKLWYSNILCGVIRGALEMLQMDVDAVFLRDILRGDEHTEIRV-HLKRI 173


>SPBC1718.05 |trs31||TRAPP complex subunit Trs31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 209

 Score = 37.5 bits (83), Expect = 0.003
 Identities = 29/133 (21%), Positives = 58/133 (43%), Gaps = 5/133 (3%)
 Frame = +1

Query: 238 VNSELLTLTYGALVSQMLKETENTEDVNKHLERIGYNMGVRLIEDFLARTTS----TRCL 405
           VN       +  L+ ++  +    ++  + L   GY +G +L+E  + R  +    TR L
Sbjct: 41  VNLSSFAFIFSELIQRIQSQVSGIQEFEEKLNEHGYRVGQKLVELVVWRERNPKRETRIL 100

Query: 406 EMRETADKIQQAFKLYLSMQPTVTSWSSAGDEFSLVWDHCPLSEWVEMPSN-NGLKYCAL 582
            + +        + L+     ++     A DE+ +V ++  L++++ +P   N L  CA 
Sbjct: 101 GILQYIHSSVWKY-LFGKHADSLEKSKEASDEYMIVDNNPLLNKFISVPKEMNQLNCCAY 159

Query: 583 IPGAIRGALQMVQ 621
           + G I G L   Q
Sbjct: 160 LAGIIEGFLDSAQ 172


>SPBC215.14c |vps20||vacuolar sorting protein
           Vps20|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 226

 Score = 31.9 bits (69), Expect = 0.13
 Identities = 29/82 (35%), Positives = 40/82 (48%), Gaps = 8/82 (9%)
 Frame = +1

Query: 160 VKKVIQILQHISTKCL------GKLLXLDAKKVNSELLTLTYGAL--VSQMLKETENTED 315
           ++K+ Q+   I+ KCL      G L  L AKK+ S L+T TYG L  + Q+L   E T  
Sbjct: 32  LEKIEQLEIDIARKCLRDSDKRGALRALKAKKLYSGLITQTYGQLGNIEQLLSTIEFTL- 90

Query: 316 VNKHLERIGYNMGVRLIEDFLA 381
           + K +   G   G  LI    A
Sbjct: 91  IQKDV-MFGLQEGTNLIRQLQA 111


>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1841

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 29/123 (23%), Positives = 47/123 (38%), Gaps = 1/123 (0%)
 Frame = +1

Query: 175  QILQHISTKCLGKLLXLDAKKVNSELLTLTYGALVSQMLKETENTEDVNKHLERIGYNMG 354
            +IL+ + T   G L  LD      E  TL            +E  +    H  ++ +   
Sbjct: 1016 KILETLHTS--GVLDELDESFAMKEAKTLVKKTCARTDYMSSELQKLFGIHFHKLSHKNP 1073

Query: 355  VRLIEDFLARTTS-TRCLEMRETADKIQQAFKLYLSMQPTVTSWSSAGDEFSLVWDHCPL 531
              +I   L    S   C+E   ++DK+    KL   ++P    W++ GD  +   D   L
Sbjct: 1074 NEIIRMILHCDDSMNECVEFL-SSDKVLNQPKLKADLEPYRIDWANGGDLVNSEKDASEL 1132

Query: 532  SEW 540
            S W
Sbjct: 1133 SRW 1135


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,102,786
Number of Sequences: 5004
Number of extensions: 59256
Number of successful extensions: 163
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)