SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP24_F_H03
         (862 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy...    77   4e-15
SPCC330.05c |ura4||orotidine 5'-phosphate decarboxylase Ura4 |Sc...    30   0.37 
SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3...    28   2.0  
SPAC144.15c |cog1||Golgi transport complex subunit Cog1 |Schizos...    27   4.5  
SPBC776.06c |||spindle pole body interacting protein |Schizosacc...    27   4.5  
SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac...    26   7.9  

>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 754

 Score = 76.6 bits (180), Expect = 4e-15
 Identities = 47/113 (41%), Positives = 63/113 (55%), Gaps = 14/113 (12%)
 Frame = +3

Query: 474 FTNIAKQYEHEFWLDMASLNVAKPLIITRVSEHINSIEKFIQRLIDNNMAYVSKNGSVYF 653
           F  +A  +E +F  DMA+LNV  P  +TRVSE++  I  F+QR+ID   AY   +GSVYF
Sbjct: 218 FRKLAAYWEDDFNKDMANLNVLPPTAVTRVSEYVPEIVDFVQRIIDRGYAYPVTDGSVYF 277

Query: 654 DTDKFSN----YGKLQ-------KMQDSGEPS---DDEKRNKMDFALWKSXKP 770
           DT+ F      Y KL+       ++   GE S      K+   DFALWK+ KP
Sbjct: 278 DTEAFEKGGHFYAKLEPWNKGNRELIAEGEGSLAALTGKKRPGDFALWKASKP 330



 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 18/40 (45%), Positives = 26/40 (65%)
 Frame = +2

Query: 191 WLMPNGNPTGIYVYNCIANQRVPVILNDPHIATWYSCGPT 310
           W +P G  T +YVYN + + +VP + N  ++ TWY CGPT
Sbjct: 11  WGVPKGQRTELYVYNTLTHSKVPFVSNGSNL-TWYCCGPT 49



 Score = 43.2 bits (97), Expect = 5e-05
 Identities = 17/40 (42%), Positives = 26/40 (65%)
 Frame = +3

Query: 306 PRXYESAHIGHASCYVKLDIIQRILKSFFNIKIVSAMGIT 425
           P  Y+++H+GHA  YV  DI++RIL+S+F   I     +T
Sbjct: 48  PTVYDASHMGHARNYVTTDILRRILQSYFGYNITFVQNVT 87


>SPCC330.05c |ura4||orotidine 5'-phosphate decarboxylase Ura4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 264

 Score = 30.3 bits (65), Expect = 0.37
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = +3

Query: 561 VSEHINSIEKFIQRLIDNNMAYVSKNGSVYFDTDKFSNYGKLQKMQ 698
           +  HI+ +E F Q +++  +A   K+  + F+  KF++ G   K+Q
Sbjct: 61  IKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVKLQ 106


>SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 378

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 11/37 (29%), Positives = 20/37 (54%)
 Frame = +3

Query: 510 WLDMASLNVAKPLIITRVSEHINSIEKFIQRLIDNNM 620
           W D+    VA+ + I    EH+N+  +F + L + N+
Sbjct: 9   WTDVKDKPVARVVFIHGFGEHVNAYPEFFEALNERNI 45


>SPAC144.15c |cog1||Golgi transport complex subunit Cog1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 701

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 16/28 (57%), Positives = 19/28 (67%)
 Frame = +1

Query: 433 MTKSLRKASKVKHTSQILLNNMSMNSGL 516
           MT SL K S V  TS ILLN  +++SGL
Sbjct: 178 MTSSLSKVSVV--TSWILLNQANISSGL 203


>SPBC776.06c |||spindle pole body interacting protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 598

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 11/32 (34%), Positives = 18/32 (56%)
 Frame = +2

Query: 122 VFNLTFVRSITHVCSLKPYKDTKWLMPNGNPT 217
           VF +    +  HV +LKP  DT W + + +P+
Sbjct: 104 VFAMAICTTFPHVHALKPLLDTAWEIFDSSPS 135


>SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 376

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 9/21 (42%), Positives = 16/21 (76%)
 Frame = +3

Query: 681 KLQKMQDSGEPSDDEKRNKMD 743
           +LQKMQD+ +  D+ +R K++
Sbjct: 296 RLQKMQDNAKELDESRRKKIE 316


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,486,291
Number of Sequences: 5004
Number of extensions: 76639
Number of successful extensions: 230
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -