Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP23_F_P08
         (1262 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   413   e-116
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   410   e-115
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   210   3e-55
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   169   7e-43
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom...    33   0.11 
SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1 |Schizosaccharo...    31   0.34 

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  413 bits (1018), Expect = e-116
 Identities = 187/266 (70%), Positives = 221/266 (83%)
 Frame = +2

Query: 137 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 316
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 317 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 496
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 497 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 676
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 677 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 856
           VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITA
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240

Query: 857 SLRFDGALNVDLTEFXTNLGALPPYP 934
           SLRF+G+LNVDL EF TN   L PYP
Sbjct: 241 SLRFEGSLNVDLAEFQTN---LVPYP 263



 Score = 55.2 bits (127), Expect = 2e-08
 Identities = 24/32 (75%), Positives = 26/32 (81%)
 Frame = +3

Query: 915  VPYPRIHFPLVTYAPXISAXKAYHEQLSVAXI 1010
            VPYPRIHFPLVTYAP +SA KA+HE  SV  I
Sbjct: 260  VPYPRIHFPLVTYAPIVSAAKAFHESNSVQEI 291



 Score = 51.6 bits (118), Expect = 2e-07
 Identities = 22/35 (62%), Positives = 24/35 (68%)
 Frame = +1

Query: 1012 TNAXXEPANQMVKCDPRSWXVXACCMLYRGDXXPR 1116
            TN   EP NQMVKCDPR+    A C+LYRGD  PR
Sbjct: 292  TNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPR 326


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  410 bits (1010), Expect = e-115
 Identities = 191/271 (70%), Positives = 223/271 (82%), Gaps = 5/271 (1%)
 Frame = +2

Query: 137 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 301
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 302 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 481
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 482 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 661
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 662 QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIV 841
           QVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+V
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239

Query: 842 SSITASLRFDGALNVDLTEFXTNLGALPPYP 934
           SSITASLRF G+LNVDL EF TN   L PYP
Sbjct: 240 SSITASLRFAGSLNVDLNEFQTN---LVPYP 267



 Score = 54.0 bits (124), Expect = 4e-08
 Identities = 23/32 (71%), Positives = 26/32 (81%)
 Frame = +3

Query: 915  VPYPRIHFPLVTYAPXISAXKAYHEQLSVAXI 1010
            VPYPRIHFPLVTY+P +SA KA+HE  SV  I
Sbjct: 264  VPYPRIHFPLVTYSPIVSAAKAFHESNSVQEI 295



 Score = 51.6 bits (118), Expect = 2e-07
 Identities = 22/35 (62%), Positives = 24/35 (68%)
 Frame = +1

Query: 1012 TNAXXEPANQMVKCDPRSWXVXACCMLYRGDXXPR 1116
            TN   EP NQMVKCDPR+    A C+LYRGD  PR
Sbjct: 296  TNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPR 330


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  210 bits (513), Expect = 3e-55
 Identities = 102/263 (38%), Positives = 148/263 (56%)
 Frame = +2

Query: 137 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 316
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 317 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 496
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 497 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 676
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+ S  
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 677 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 856
           VVEPYN+ L+ H  +E+SD  F +DNEA+  I    L I+ P+Y +LN L+  +++ +T 
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTT 238

Query: 857 SLRFDGALNVDLTEFXTNLGALP 925
           S RF G LN DL +   N+   P
Sbjct: 239 SFRFPGELNSDLRKLAVNMVPFP 261



 Score = 29.1 bits (62), Expect = 1.4
 Identities = 11/35 (31%), Positives = 22/35 (62%)
 Frame = +3

Query: 915  VPYPRIHFPLVTYAPXISAXKAYHEQLSVAXIXKR 1019
            VP+PR+HF +V +AP  +   +  + +SV  + ++
Sbjct: 258  VPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQ 292


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  169 bits (411), Expect = 7e-43
 Identities = 91/261 (34%), Positives = 145/261 (55%), Gaps = 3/261 (1%)
 Frame = +2

Query: 140 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 319
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 320 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVL 493
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 494 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 670
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 671 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSI 850
             VV+PYNS+L       ++D   ++DN A+  I    L  + PT+   N+L+  ++S+ 
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239

Query: 851 TASLRFDGALNVDLTEFXTNL 913
           T +LR+ G +N DL     +L
Sbjct: 240 TTTLRYPGYMNNDLVSIIASL 260


>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
           Msp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 903

 Score = 32.7 bits (71), Expect = 0.11
 Identities = 17/48 (35%), Positives = 28/48 (58%)
 Frame = -2

Query: 901 ELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 758
           E  + H  ++ + Q+SSD  ++ +D T  VG+G AL   I + D +DG
Sbjct: 171 ERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216


>SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 399

 Score = 31.1 bits (67), Expect = 0.34
 Identities = 30/114 (26%), Positives = 47/114 (41%), Gaps = 6/114 (5%)
 Frame = +2

Query: 647 IYPAPQ----VSTAVVEPYNSILTTHTTLEHSDCAF--MVDNEAIYDICRRNLDIERPTY 808
           I+P PQ     +  +++   SIL  H  L      F  + D E I  + R+NL  E   +
Sbjct: 151 IFPDPQKTLDYAKMILKAGASILAVHGRLREQKGHFTGIADWEQI-QMLRKNLPSETVLF 209

Query: 809 TNLNRLIGQIVSSITASLRFDGALNVDLTEFXTNLGALPPYPLPTGHVRXXHLC 970
            N N L  Q +         DG L+ + + +   +   P  PL T + R   +C
Sbjct: 210 ANGNILHAQDIDRCIKYTGVDGVLSAEGSLYNPRIFLPPSSPLMTLYPRIDDMC 263


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,566,424
Number of Sequences: 5004
Number of extensions: 90083
Number of successful extensions: 264
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 244
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 259
length of database: 2,362,478
effective HSP length: 75
effective length of database: 1,987,178
effective search space used: 685576410
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)