Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP20_F_N23
         (881 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   180   3e-46
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   170   3e-43
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    93   6e-20
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    88   2e-18
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   8.2  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   8.2  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  180 bits (437), Expect = 3e-46
 Identities = 78/106 (73%), Positives = 89/106 (83%)
 Frame = +2

Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 463
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARG
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARG 106



 Score = 60.5 bits (140), Expect = 3e-10
 Identities = 23/33 (69%), Positives = 30/33 (90%)
 Frame = +3

Query: 465 HYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 563
           HYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 107 HYTVGKELVDEVTDKIRRIADNCSGLQGFLVFH 139



 Score = 59.7 bits (138), Expect = 5e-10
 Identities = 25/37 (67%), Positives = 34/37 (91%)
 Frame = +1

Query: 601 LLMERLSVDYGXKSKLEFAIYPAPQVSTAVVEPXNSI 711
           LL+ERL+++Y  KSKL+F++YPAPQVST+VVEP NS+
Sbjct: 152 LLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSV 188


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  170 bits (413), Expect = 3e-43
 Identities = 79/111 (71%), Positives = 90/111 (81%), Gaps = 5/111 (4%)
 Frame = +2

Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 310
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 311 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 463
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARG
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARG 110



 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 23/33 (69%), Positives = 31/33 (93%)
 Frame = +3

Query: 465 HYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 563
           HYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 111 HYTVGKEMIDSVLERIRRMADNCSGLQGFLVFH 143



 Score = 60.9 bits (141), Expect = 2e-10
 Identities = 25/37 (67%), Positives = 34/37 (91%)
 Frame = +1

Query: 601 LLMERLSVDYGXKSKLEFAIYPAPQVSTAVVEPXNSI 711
           LL+ERL+++YG KS L+F++YPAPQVST+VVEP NS+
Sbjct: 156 LLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSV 192


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 92.7 bits (220), Expect = 6e-20
 Identities = 45/110 (40%), Positives = 67/110 (60%), Gaps = 2/110 (1%)
 Frame = +2

Query: 149 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 328
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 329 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGSLH 472
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G  H
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSH 110


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 87.8 bits (208), Expect = 2e-18
 Identities = 44/113 (38%), Positives = 63/113 (55%)
 Frame = +2

Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGSLHHWKG 484
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+G  H+ +G
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG--HYTEG 109



 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 17/33 (51%), Positives = 22/33 (66%)
 Frame = +3

Query: 465 HYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 563
           HYT G E+ D VLD +R+ A+ C  LQGF + H
Sbjct: 105 HYTEGAELADAVLDVVRREAEACDALQGFQLTH 137



 Score = 31.9 bits (69), Expect = 0.12
 Identities = 13/36 (36%), Positives = 22/36 (61%)
 Frame = +1

Query: 601 LLMERLSVDYGXKSKLEFAIYPAPQVSTAVVEPXNS 708
           LL+ ++  +Y  +    F++ PAP+ S  VVEP N+
Sbjct: 150 LLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNA 185


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 8.2
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 225 STASSLMARCPQTRPSGVETILSTLSSARPELAST 329
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 8.2
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 244 WPDAHRQDHRGWRRFFQHFLQR 309
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,126,511
Number of Sequences: 5004
Number of extensions: 61692
Number of successful extensions: 167
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)