Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP20_F_I08
(894 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 123 3e-29
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 123 3e-29
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 123 3e-29
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 68 2e-12
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 57 4e-09
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 45 1e-05
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 4e-05
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 38 0.001
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 27 2.7
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 26 6.3
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 26 8.3
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 26 8.3
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 8.3
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 123 bits (297), Expect = 3e-29
Identities = 57/72 (79%), Positives = 62/72 (86%)
Frame = +2
Query: 203 KCGGXXKRTIEXVRDRRPRKMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVT 382
KCGG KRTIE ++ ++GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT
Sbjct: 30 KCGGIDKRTIEKF-EKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVT 88
Query: 383 IIDAPGHRDFIK 418
+IDAPGHRDFIK
Sbjct: 89 VIDAPGHRDFIK 100
Score = 92.3 bits (219), Expect = 8e-20
Identities = 43/65 (66%), Positives = 54/65 (83%)
Frame = +1
Query: 535 LAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 714
LA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ G
Sbjct: 139 LAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQG 196
Query: 715 DNMLE 729
DNM+E
Sbjct: 197 DNMIE 201
Score = 76.2 bits (179), Expect = 6e-15
Identities = 33/42 (78%), Positives = 38/42 (90%)
Frame = +3
Query: 417 RNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALARF 542
+NMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHAL +
Sbjct: 100 KNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAY 141
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 123 bits (297), Expect = 3e-29
Identities = 57/72 (79%), Positives = 62/72 (86%)
Frame = +2
Query: 203 KCGGXXKRTIEXVRDRRPRKMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVT 382
KCGG KRTIE ++ ++GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT
Sbjct: 30 KCGGIDKRTIEKF-EKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVT 88
Query: 383 IIDAPGHRDFIK 418
+IDAPGHRDFIK
Sbjct: 89 VIDAPGHRDFIK 100
Score = 92.3 bits (219), Expect = 8e-20
Identities = 43/65 (66%), Positives = 54/65 (83%)
Frame = +1
Query: 535 LAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 714
LA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ G
Sbjct: 139 LAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQG 196
Query: 715 DNMLE 729
DNM+E
Sbjct: 197 DNMIE 201
Score = 76.6 bits (180), Expect = 4e-15
Identities = 34/42 (80%), Positives = 38/42 (90%)
Frame = +3
Query: 417 RNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALARF 542
+NMITGTSQADCAVLI+ GTGEFEAGISK+GQTREHAL +
Sbjct: 100 KNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAY 141
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 123 bits (297), Expect = 3e-29
Identities = 57/72 (79%), Positives = 62/72 (86%)
Frame = +2
Query: 203 KCGGXXKRTIEXVRDRRPRKMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVT 382
KCGG KRTIE ++ ++GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT
Sbjct: 30 KCGGIDKRTIEKF-EKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVT 88
Query: 383 IIDAPGHRDFIK 418
+IDAPGHRDFIK
Sbjct: 89 VIDAPGHRDFIK 100
Score = 92.3 bits (219), Expect = 8e-20
Identities = 43/65 (66%), Positives = 54/65 (83%)
Frame = +1
Query: 535 LAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 714
LA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++IKK+G+NP V FVP+SG+ G
Sbjct: 139 LAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQG 196
Query: 715 DNMLE 729
DNM+E
Sbjct: 197 DNMIE 201
Score = 76.2 bits (179), Expect = 6e-15
Identities = 33/42 (78%), Positives = 38/42 (90%)
Frame = +3
Query: 417 RNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALARF 542
+NMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHAL +
Sbjct: 100 KNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAY 141
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 68.1 bits (159), Expect = 2e-12
Identities = 30/50 (60%), Positives = 36/50 (72%)
Frame = +2
Query: 266 GKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFI 415
GKGSF YAW+LD + ER RG+T+D+A FE+ K I DAPGHRDFI
Sbjct: 220 GKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHRDFI 269
Score = 54.4 bits (125), Expect = 2e-08
Identities = 28/67 (41%), Positives = 43/67 (64%), Gaps = 1/67 (1%)
Frame = +1
Query: 532 WLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY-IKKIGYNPAAVAFVPISGW 708
+L LG+ +++V VNK+D +SE RF+EIK VS + IK +G+ + V FVPIS
Sbjct: 308 YLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAI 365
Query: 709 HGDNMLE 729
G N+++
Sbjct: 366 SGTNLIQ 372
Score = 45.6 bits (103), Expect = 1e-05
Identities = 21/36 (58%), Positives = 24/36 (66%)
Frame = +3
Query: 423 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHA 530
MI G S AD AVL+V + FE G +NGQTREHA
Sbjct: 272 MIAGASSADFAVLVVDSSQNNFERGFLENGQTREHA 307
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 56.8 bits (131), Expect = 4e-09
Identities = 25/69 (36%), Positives = 42/69 (60%)
Frame = +2
Query: 209 GGXXKRTIEXVRDRRPRKMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTII 388
G KRT+E + +R ++ GK S+ +W LD ERE+G T+++ FET +++
Sbjct: 263 GMVDKRTMEKI-EREAKEAGKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLL 321
Query: 389 DAPGHRDFI 415
DAPGH+ ++
Sbjct: 322 DAPGHKGYV 330
Score = 56.0 bits (129), Expect = 7e-09
Identities = 27/41 (65%), Positives = 31/41 (75%), Gaps = 1/41 (2%)
Frame = +3
Query: 420 NMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHA-LAR 539
NMI G SQAD VL+++A GEFEAG + GQTREHA LAR
Sbjct: 332 NMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLAR 372
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 45.2 bits (102), Expect = 1e-05
Identities = 21/52 (40%), Positives = 31/52 (59%)
Frame = +2
Query: 263 MGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIK 418
+G+ SF +DK E+ RGITI A ++ET+ + +D PGH D+IK
Sbjct: 80 LGQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIK 131
Score = 32.7 bits (71), Expect = 0.072
Identities = 16/39 (41%), Positives = 25/39 (64%)
Frame = +1
Query: 535 LAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 651
LA +GVKQ++V +NK+D EP E E+++ +S Y
Sbjct: 163 LARQVGVKQIVVYINKVDMVEPDMIELVEMEMRELLSEY 201
Score = 29.5 bits (63), Expect = 0.67
Identities = 16/39 (41%), Positives = 23/39 (58%)
Frame = +3
Query: 417 RNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 533
+NMITG + D A+++V+A G+ QTREH L
Sbjct: 131 KNMITGAATMDGAIIVVSATDGQMP-------QTREHLL 162
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 43.6 bits (98), Expect = 4e-05
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +2
Query: 293 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 412
V+D L AER+RGITI+ A F + +ID PGH DF
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADF 106
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 38.3 bits (85), Expect = 0.001
Identities = 18/43 (41%), Positives = 25/43 (58%), Gaps = 4/43 (9%)
Frame = +2
Query: 296 LDKLKAERERGITIDI----ALWKFETSKYYVTIIDAPGHRDF 412
LDKL+ ER RGIT+ ++ + Y + +ID PGH DF
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDF 137
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/42 (33%), Positives = 26/42 (61%), Gaps = 3/42 (7%)
Frame = +2
Query: 299 DKLKAER-ERGITIDIALWKFETSKYYV--TIIDAPGHRDFI 415
+K++A+ E+ + I+I + E +++ T+ID PG DFI
Sbjct: 60 EKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFI 101
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 26.2 bits (55), Expect = 6.3
Identities = 22/58 (37%), Positives = 28/58 (48%), Gaps = 19/58 (32%)
Frame = +2
Query: 296 LDKLKAERERGITIDIA----LWK---------------FETSKYYVTIIDAPGHRDF 412
+D ++ ERE+GITI A W+ FE S Y + IID PGH DF
Sbjct: 102 MDFMELEREKGITIQSAATHCTWERTVDQIEANEKQKTDFEKS-YNINIIDTPGHIDF 158
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 25.8 bits (54), Expect = 8.3
Identities = 20/70 (28%), Positives = 29/70 (41%)
Frame = +1
Query: 505 RTVKPVSMPWLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAV 684
R KP+ P + F G +I ++ + P + P EVS I +P
Sbjct: 1530 RNGKPIEQP-VEFENGGYSVIQVMD--EGYSPVFVTPPTNSPYAEVSGDYNPIHVSPTFA 1586
Query: 685 AFVPISGWHG 714
AFV + G HG
Sbjct: 1587 AFVELPGTHG 1596
>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1253
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/40 (30%), Positives = 23/40 (57%)
Frame = -2
Query: 632 LISSNLGSLYGGSVESILFTPTMSCLTPRVKASQGMLTGL 513
+I +L S YG + + T + P+++A+ GM++GL
Sbjct: 772 IIREHLTSKYGDTHFQAIITSYLCESPPKIEAALGMISGL 811
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/43 (25%), Positives = 21/43 (48%)
Frame = +2
Query: 341 IALWKFETSKYYVTIIDAPGHRDFIKKHDHRNLSG*LRCAHRS 469
+ +W F T + + + ++ R F + DHR + + C H S
Sbjct: 451 VRIWNFNTGEQHCVLHNSRNSRVFGLQFDHRRI---IACTHSS 490
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,964,367
Number of Sequences: 5004
Number of extensions: 57798
Number of successful extensions: 186
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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