Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fdpeP20_F_A11
         (891 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   266   4e-72
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   258   6e-70
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   132   5e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   118   1e-27
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   8.3  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   8.3  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  266 bits (651), Expect = 4e-72
 Identities = 116/174 (66%), Positives = 141/174 (81%)
 Frame = +2

Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 505
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 506 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 667
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPA
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPA 174



 Score = 79.8 bits (188), Expect = 5e-16
 Identities = 37/74 (50%), Positives = 46/74 (62%)
 Frame = +1

Query: 661 PRASGSTAVVEPYNSIXXXXXXXXXSDCAFMVDNEAIYDICRRNLDIERPTYTNLKSVSS 840
           P    ST+VVEPYNS+         +DC FMVDNE+ YDICRRNLDIERP+Y NL  + +
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIA 232

Query: 841 DRXSPRITALEIDG 882
              S    +L  +G
Sbjct: 233 QVVSSITASLRFEG 246


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  258 bits (633), Expect = 6e-70
 Identities = 117/179 (65%), Positives = 143/179 (79%), Gaps = 5/179 (2%)
 Frame = +2

Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 310
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 311 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 490
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 491 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 667
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPA
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178



 Score = 82.2 bits (194), Expect = 9e-17
 Identities = 40/74 (54%), Positives = 45/74 (60%)
 Frame = +1

Query: 661 PRASGSTAVVEPYNSIXXXXXXXXXSDCAFMVDNEAIYDICRRNLDIERPTYTNLKSVSS 840
           P    ST+VVEPYNS+         SDC FMVDNEA YDICRRNLDIERPTY NL  + +
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIA 236

Query: 841 DRXSPRITALEIDG 882
              S    +L   G
Sbjct: 237 QVVSSITASLRFAG 250


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  132 bits (320), Expect = 5e-32
 Identities = 65/174 (37%), Positives = 94/174 (54%)
 Frame = +2

Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 505
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 506 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 667
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PA
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPA 172



 Score = 42.7 bits (96), Expect = 7e-05
 Identities = 22/60 (36%), Positives = 29/60 (48%)
 Frame = +1

Query: 661 PRASGSTAVVEPYNSIXXXXXXXXXSDCAFMVDNEAIYDICRRNLDIERPTYTNLKSVSS 840
           P    S  VVEPYN+          SD  F +DNEA+  I    L I+ P+Y +L  + S
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVS 230


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  118 bits (283), Expect = 1e-27
 Identities = 62/174 (35%), Positives = 98/174 (56%), Gaps = 2/174 (1%)
 Frame = +2

Query: 149 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 328
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 329 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 502
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 503 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP 664
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173



 Score = 36.3 bits (80), Expect = 0.006
 Identities = 21/71 (29%), Positives = 30/71 (42%)
 Frame = +1

Query: 670 SGSTAVVEPYNSIXXXXXXXXXSDCAFMVDNEAIYDICRRNLDIERPTYTNLKSVSSDRX 849
           S S  VV+PYNS+         +D   ++DN A+  I    L  + PT+     + S   
Sbjct: 177 SVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVM 236

Query: 850 SPRITALEIDG 882
           S   T L   G
Sbjct: 237 SASTTTLRYPG 247


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 225 STASSLMARCPQTRPSGVETILSTLSSARPELAST 329
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 244 WPDAHRQDHRGWRRFFQHFLQR 309
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,586,756
Number of Sequences: 5004
Number of extensions: 74986
Number of successful extensions: 240
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 236
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)