BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fdpeP17_F_F22
(850 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|ch... 62 1e-10
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 27 2.5
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 2.5
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 27 2.5
SPAC9E9.10c |cbh1|cbh|centromere binding protein |Schizosaccharo... 27 2.5
SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protei... 27 3.4
SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce... 26 5.9
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 26 5.9
>SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 548
Score = 61.7 bits (143), Expect = 1e-10
Identities = 49/154 (31%), Positives = 81/154 (52%), Gaps = 10/154 (6%)
Frame = +1
Query: 226 MCGIFLELSCEKCSIVND--IDVLR-RLKNRGPDHLQRKDFISEIGQHIVFVASVLWMQG 396
MCGI L+ ++ +++ LR R++ RGPD K ++ ++ F +SVL ++G
Sbjct: 1 MCGILFALAEDRDNLLESPSFSALRKRIRARGPDFFG-KHKLNSGPWNLHFESSVLHLRG 59
Query: 397 PQ--MTLQPLENECG-ILLYNGDIFDESWDSRTSDTQIIME----KLSYDCDSQIDKVIN 555
P +T QP + G +L +NG+I+ + T E KL ++ +
Sbjct: 60 PSDHLTPQPHVDSFGNVLCWNGEIWQINHSDHHKFTLNRNENDGAKLFELLNNNPGDIEK 119
Query: 556 VLKSLKGPFSLIYYCKVTNXLYFTRDRFGRNSLL 657
+L S++GPF+ +YY TN L++ RDR GR SLL
Sbjct: 120 ILGSIQGPFAFVYYQVRTNTLWWGRDRLGRRSLL 153
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 27.5 bits (58), Expect = 2.5
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = -2
Query: 300 ESSKDINIVHYATFFAA*LQKYTTHAVNYGIQTINSNSKF 181
E S D N +H + FAA ++ TH + +G+ T + +F
Sbjct: 1571 EVSGDYNPIHVSPTFAAFVELPGTHGITHGMYTSAAARRF 1610
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 27.5 bits (58), Expect = 2.5
Identities = 15/71 (21%), Positives = 34/71 (47%)
Frame = +1
Query: 355 QHIVFVASVLWMQGPQMTLQPLENECGILLYNGDIFDESWDSRTSDTQIIMEKLSYDCDS 534
Q+I+ A + + T + +EN Y+ ++ D+ W S + ++++ C S
Sbjct: 675 QYIMNAAQDILRRRFHATSEQVENCVKPYKYDVEVNDDEWKSSRGQAEKLLQRELGLCQS 734
Query: 535 QIDKVINVLKS 567
++K+ N + S
Sbjct: 735 ALEKIKNAVGS 745
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 27.5 bits (58), Expect = 2.5
Identities = 22/81 (27%), Positives = 39/81 (48%), Gaps = 8/81 (9%)
Frame = -3
Query: 248 NSKNIP----HMLSITVSKRST-AIQSFKWFKNTTDVA*TCVSNASDITRVILLYDVMQH 84
+SK++P H+ S K S+ ++ + + TTD+ + SD+TR LL + +
Sbjct: 158 SSKDVPTASAHISSSDHEKSSSVSLSALNNYNKTTDIKARSLDRLSDMTRPKLLLNTKRS 217
Query: 83 HTLRE---GNKTINNSNLKDS 30
H E + + + LKDS
Sbjct: 218 HRSSEEPGASSPVTSPILKDS 238
>SPAC9E9.10c |cbh1|cbh|centromere binding protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 514
Score = 27.5 bits (58), Expect = 2.5
Identities = 21/64 (32%), Positives = 34/64 (53%), Gaps = 2/64 (3%)
Frame = +1
Query: 382 LWMQGPQMTLQPLENECGILLYNGDIFDESWDSRTSDTQIIMEKLS--YDCDSQIDKVIN 555
LW+ G + E +CGI + + F+ W+ R S + IIME+ +D Q KV+
Sbjct: 226 LWIVGYAQNPRSFE-QCGIFPKSMN-FEWKWNGRASISPIIMEEWLRWFDTRMQGRKVLL 283
Query: 556 VLKS 567
+L+S
Sbjct: 284 MLES 287
>SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1462
Score = 27.1 bits (57), Expect = 3.4
Identities = 17/45 (37%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = -3
Query: 302 LSLRRTSISFTMLHFSQLN-SKNIPHMLSITVSKRSTAIQSFKWF 171
L R +I + HF+ K++ H LS T T IQ FKWF
Sbjct: 104 LQKRVLNILIDLWHFNPSEIEKSLIH-LSTTSKSAETRIQCFKWF 147
>SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 26.2 bits (55), Expect = 5.9
Identities = 11/42 (26%), Positives = 19/42 (45%)
Frame = +1
Query: 343 SEIGQHIVFVASVLWMQGPQMTLQPLENECGILLYNGDIFDE 468
S+ H+ + V+W+ PQ LE EC + N ++
Sbjct: 242 SDNNSHLYGICVVVWVAMPQSMQNDLEKECEVWRANNTTVED 283
>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1010
Score = 26.2 bits (55), Expect = 5.9
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = -2
Query: 477 IPRFVKYITIIQKDATFIFERL*CHLRTLH 388
+P FVK++ ++ DAT++ + L +H
Sbjct: 723 LPFFVKFVALMLNDATYLLDEALLKLTEIH 752
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,139,877
Number of Sequences: 5004
Number of extensions: 62382
Number of successful extensions: 171
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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